LOCUS       Exported                6145 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..3110
                     /label=YAP1
                     /note="YAP1"
     misc_feature    1969..1974
                     /label=EcoICR I
     misc_feature    2007..2013
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2248..2253
                     /label=6base
     misc_feature    complement(2797..2802)
                     /label=6base
     misc_feature    complement(2955..2960)
                     /label=6base
     misc_feature    3111..3118
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3270..3491
                     /label=Sv40 late polyadenylation signal
     promoter        3590..4008
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4016..4082
                     /label=EM7 bacterial promoter
     CDS             4096..4890
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4954..5002
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    5045..5967
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    5222
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggatcccggg cagcagccgc
     1621 cgcctcaacc ggccccccag ggccaagggc agccgccttc gcagcccccg caggggcagg
     1681 gcccgccgtc cggacccggg caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac
     1741 cccccgccgg gcatcagatc gtgcacgtcc gcggggactc ggagaccgac ctggaggcgc
     1801 tcttcaacgc cgtcatgaac cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc
     1861 tccggaagct gcccgactcc ttcttcaagc cgccggagcc caaatcccac tcccgacagg
     1921 ccagtactga tgcaggcact gcaggagccc tgactccaca gcatgttcga gctcattcct
     1981 ctccagcttc tctgcagttg ggagctgttt ctcctgggac actgaccccc actggagtag
     2041 tctctggccc agcagctaca cccacagctc agcatcttcg acagtcttct tttgagatac
     2101 ctgatgatgt acctctgcca gcaggttggg agatggcaaa gacatcttct ggtcagagat
     2161 acttcttaaa tcacatcgat cagacaacaa catggcagga ccccaggaag gccatgctgt
     2221 cccagatgaa cgtcacagcc cccaccagtc caccagtgca gcagaatatg atgaactcgg
     2281 cttcaggtcc tcttcctgat ggatgggaac aagccatgac tcaggatgga gaaatttact
     2341 atataaacca taagaacaag accacctctt ggctagaccc aaggcttgac cctcgttttg
     2401 ccatgaacca gagaatcagt cagagtgctc cagtgaaaca gccaccaccc ctggctcccc
     2461 agagcccaca gggaggcgtc atgggtggca gcaactccaa ccagcagcaa cagatgcgac
     2521 tgcagcaact gcagatggag aaggagaggc tgcggctgaa acagcaagaa ctgcttcggc
     2581 aggcaatgcg gaatatcaat cccagcacag caaattctcc aaaatgtcag gagttagccc
     2641 tgcgtagcca gttaccaaca ctggagcagg atggtgggac tcaaaatcca gtgtcttctc
     2701 ccgggatgtc tcaggaattg agaacaatga cgaccaatag ctcagatcct ttccttaaca
     2761 gtggcaccta tcactctcga gatgagagta cagacagtgg actaagcatg agcagctaca
     2821 gtgtccctcg aaccccagat gacttcctga acagtgtgga tgagatggat acaggtgata
     2881 ctatcaacca aagcaccctg ccctcacagc agaaccgttt cccagactac cttgaagcca
     2941 ttcctgggac aaatgtggac cttggaacac tggaaggaga tggaatgaac atagaaggag
     3001 aggagctgat gccaagtctg caggaagctt tgagttctga catccttaat gacatggagt
     3061 ctgttttggc tgccaccaag ctagataaag aaagctttct tacatggtta gtttaaacga
     3121 attcgggctc ggtacccggg gatcctctag agtcgacctg caggcatgca agctgatccg
     3181 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     3241 gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     3301 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3361 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     3421 ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta
     3481 caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     3541 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca
     3601 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     3661 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3721 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3781 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3841 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3901 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3961 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttaa ttaactgttg
     4021 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     4081 cccaggaggc agatcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4141 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4201 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4261 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4321 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4381 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4441 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4501 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4561 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4621 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4681 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4741 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4801 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4861 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     4921 aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt ttcattacat
     4981 ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tccgctcatg cgatgataag
     5041 ctgtcaaaca tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg
     5101 aagagataaa ttgcactgaa atctagaaat attttatctg attaataaga tgatcttctt
     5161 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
     5221 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
     5281 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
     5341 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
     5401 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
     5461 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
     5521 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     5581 agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5641 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg
     5701 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc
     5761 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt
     5821 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc
     5881 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa
     5941 catagtaagc cagtatacac tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg
     6001 caccaccccg tcatgagatt acgcgtttta gcggtgcgta caattaaggg attatggtaa
     6061 atccacttac tgtctgccct cgtagccatc gagataaacc gcagtactcc ggccacgatg
     6121 cgtccggcgt agaggatcga gatct
//
