LOCUS       Exported                5139 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..1464
                     /label=CBPbd
     misc_feature    1467..1481
                     /label=linker
                     /note="linker"
     misc_feature    1482..1991
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    2128..2349
                     /label=Sv40 late polyadenylation signal
     promoter        2448..2866
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        2874..2940
                     /label=EM7 bacterial promoter
     CDS             2954..3748
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    3812..3860
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      4096..4132
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcgc aaaaaaatct
     1081 ttaaaccaga ggagttacgc caggccctca tgccaaccct agaagcactg tatcgacagg
     1141 acccagagtc attacctttc cggcagcctg tagatcccca gctcctcgga attccagact
     1201 attttgacat cgtaaagaat cccatggacc tctccaccat caagcggaag ctggacacag
     1261 ggcaatacca agagccctgg cagtacgtgg acgacgtctg gctcatgttc aacaatgcct
     1321 ggctctataa tcgcaagaca tcccgagtct ataagttttg cagtaagctt gcagaggtct
     1381 ttgagcagga aattgaccct gtcatgcagt cccttggata ttgctgtgga cgcaagtatg
     1441 agttttcccc acagactttg gtttctctcg gctcgagcgg cgtcttcaca ctcgaagatt
     1501 tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag
     1561 gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc
     1621 tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
     1681 gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc
     1741 atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca
     1801 tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
     1861 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg
     1921 acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac
     1981 gcattctggc gtaaggccgc gactctagag tcgacctgca ggcatgcaag ctgatccggc
     2041 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     2101 ataacccctt ggggcggccg cttcgagcag acatgataag atacattgat gagtttggac
     2161 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2221 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     2281 ttatgtttca ggttcagggg gagatgtggg aggttttttt aagcaagtaa aacctctaca
     2341 aatgtggtaa aatcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     2401 ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg cagcaccatg
     2461 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
     2521 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     2581 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     2641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2761 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     2821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttaatt aactgttgac
     2881 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2941 caggaggcag atcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3001 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3061 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3121 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3181 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3241 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3301 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3361 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3421 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     3481 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3541 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3601 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     3661 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     3721 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     3781 gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt cattacatct
     3841 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc ctctttgcgc ttgcgttttc
     3901 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
     3961 ggctttctac ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4021 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4081 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4501 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4681 atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata
     4801 gtccggaaat acaggaacgc acgctggatg gcccttcgct gggatggtga aaccatgaaa
     4861 aatggcagct tcagtggatt aagtgggggt aatgtggcct gtaccctctg gttgcatagg
     4921 tattcatacg gttaaaattt atcaggcgcg attgcggcag tttttcgggt ggtttgttgc
     4981 catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg cgtacaatta
     5041 agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata aaccgcagta
     5101 ctccggccac gatgcgtccg gcgtagagga tcgagatct
//
