LOCUS       Exported                7272 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC32K(pF-Nc5K3)      5119 bp    DNA   circular         
              12/7/2015

            DEFINITION  Current Name: pFC32K (pF-Nc5K3) Old/Alternate 
            Name(s):pF-Nc5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..3593
                     /label=TTC7B
                     /note="TTC7B"
     misc_feature    3598..3600
                     /label=EcoICR I
     misc_feature    3600..3614
                     /label=C Terminal NanoLuc linker
     misc_feature    3603..3614
                     /label=N Terminal NanoLuc linker
     CDS             3615..4124
                     /label=C terminal NanoLuc
     polyA_signal    4261..4482
                     /label=Sv40 late polyadenylation signal
     promoter        4581..4999
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(4654..4890)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      4897..4962
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        5007..5073
                     /label=EM7 bacterial promoter
     CDS             5087..5881
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5945..5993
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      6229..6265
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6936..7221
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggcg accaagaagg
     1081 caggctcgcg gctggagacg gagatcgagc gctgccgctc cgagtgccag tgggagcgga
     1141 tccctgagct cgtcaagcag ctgtcggcca agctcatcgc caacgatgac atggcagagc
     1201 ttctcctcgg ggagtcgaag ctggagcagt acctgaagga acaccccctg aggcaggggg
     1261 ccagtccccg aggccccaag ccccagctga ctgaggtccg caagcatctg accgccgccc
     1321 tggaccgagg gaaccttaag tcagaattcc tacaagaatc caatctgatc atggccaagt
     1381 tgaattatgt ggaaggtgat tataaagaag ctctgaacat ttacgcccgg gtgggcctgg
     1441 acgatctgcc actgacagct gtcccgccct acaggctgcg ggtgatcgca gaagcctacg
     1501 ctaccaaagg actttgtttg gagaagctgc ctatttcttc ttctaccagt aatctccatg
     1561 tggaccggga acaggatgtc atcacctgtt atgagaaagc aggggacatc gcactcctgt
     1621 atctccaaga gatagaaagg gtaatacttt ctaatattca aaacagaagc cctaagcctg
     1681 gccctgctcc ccacgatcaa gaactaggtt ttttcctaga aacaggactt cagagagccc
     1741 atgtcctcta tttcaaaaat gggaacttga caagaggagt cggaagattt agagagcttc
     1801 tcagagcagt tgaaacaaga acgactcaaa acctgcgaat gacaatagcc aggcagctgg
     1861 cagagatctt gttgcggggt atgtgtgagc agagctactg gaaccctctg gaggatccac
     1921 cgtgccagtc acctctggac gatcctctcc gcaaaggagc aaacacaaaa acctacactc
     1981 tcactcggag agcccgtgtc tactcaggag agaacatttt ttgtcctcaa gaaaatacgg
     2041 aagaagccct gttgttattg ctgattagtg aatcaatggc caaccgggac gctgtgctga
     2101 gcaggatacc tgaacacaag agtgaccgcc tcatcagtct gcagagtgca tctgtggtct
     2161 atgacttact caccattgct cttggaagaa gaggccagta tgagatgctg tcagagtgcc
     2221 tagaaagagc catgaagttt gcctttgagg aattccacct gtggtaccag tttgctctgt
     2281 ccctgatggc tgctggaaaa tctgcccgtg ccgtgaaggt gctgaaagag tgtatccgcc
     2341 tgaagccaga cgatgccacc atccctctcc tcgctgccaa gctctgcatg ggctccctgc
     2401 actggttgga agaggctgaa aagtttgcca aaactgtcgt tgatgtggga gagaaaacgt
     2461 cagagttcaa ggccaaaggc tacttagctc tggggctcac gtacagtctg caggccactg
     2521 acgcttcttt gcgagggatg caggaggtcc tacagagaaa ggcgcttctt gcatttcaga
     2581 gggcccacag cctgtcaccc acagatcacc aagcagcttt ctacctggct ctgcagcttg
     2641 ccatctccag acagatccca gaggctctgg ggtatgtccg ccaagctctt cagcttcaag
     2701 gtgacgatgc caactccctg cacctccttg ccctcctgct gtcagcacag aagcattacc
     2761 atgacgctct gaacatcatc gacatggccc tgagtgaata cccagaaaat ttcatactac
     2821 tgttttccaa agtgaagttg cagtcactct gccgaggccc ggacgaggca ctgctgactt
     2881 gtaagcacat gctgcagata tggaaatcct gctacaacct caccaacccc agtgattctg
     2941 gacgtgggag cagcctctta gatagaacca ttgctgacag acgacagctt aatacaatta
     3001 ctttgccaga cttcagcgat cccgagacag gctccgtcca tgccacatcg gtagcagcct
     3061 caagagtgga gcaggcactg tcggaagtgg cttcgtctct gcagagcagt gcccctaagc
     3121 agggcccgct gcacccctgg atgacgctgg cacagatctg gctccatgca gctgaagtct
     3181 atatcggcat cgggaagcct gcagaagcca cagcctgtac ccaagaagct gccaacctct
     3241 tcccaatgtc ccacaatgtc ctctacatgc gcggccagat tgctgagctc cggggaagca
     3301 tggacgaggc gcggcggtgg tatgaagagg ccttagccat cagccccacc cacgtgaaga
     3361 gcatgcagcg actggccctg atccttcacc agctaggccg ctacagtctg gcggagaaga
     3421 tcctccggga cgcggtgcag gtgaactcga cagcccacga ggtctggaac gggctgggcg
     3481 aggtcctcca agctcagggc aacgatgcgg cggctacgga gtgcttcctg acagccttgg
     3541 agctggaggc cagcagcccc gccgtgccct tcaccatcat cccccgcgtg ctcgtttctc
     3601 tcggctcgag cggcgtcttc acactcgaag atttcgttgg ggactggcga cagacagccg
     3661 gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg
     3721 gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg
     3781 acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa
     3841 aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg
     3901 gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg
     3961 aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca
     4021 acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca
     4081 tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcgtaaggc cgcgactcta
     4141 gagtcgacct gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga
     4201 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag
     4261 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     4321 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     4381 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     4441 gggaggtttt tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa
     4501 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4561 cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     4621 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4681 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4741 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4801 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4861 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4921 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4981 aggcttttgc aaaaagctta attaactgtt gacaattaat catcggcata gtatatcggc
     5041 atagtataat acgacaaggt gaggaactaa acccaggagg cagatcatga ttgaacaaga
     5101 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5161 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5221 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5281 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5341 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5401 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5461 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5521 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5581 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     5641 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5701 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5761 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5821 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5881 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg
     5941 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     6001 agcgataagg atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     6061 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacccggtat cagctcactc
     6121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6661 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6781 ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc
     6841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6901 atcaaaaagg atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg
     6961 atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg
     7021 ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc
     7081 gcgattgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     7141 gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt
     7201 ctgccctcgt agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga
     7261 ggatcgagat ct
//
