LOCUS       Exported                7298 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)      5128 bp    DNA   circular         
              9/3/2015

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) Old/Alternate 
            Name(s):pF-Nn5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1577
                     /label=NanoLuc
                     /note="N Terminal version. Renamed from 'N terminal 
                     NanoLuc' Feb 2015"
     CDS             1068..1577
                     /label=NanoLuc
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
     CDS             1068..1577
                     /label=NanoLuc
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..4127
                     /label=TTC7B
                     /note="TTC7B"
     misc_feature    4128..4135
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4132..4265
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    4287..4508
                     /label=Sv40 late polyadenylation signal
     promoter        4607..5025
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(4680..4916)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      4923..4988
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        5033..5099
                     /label=EM7 bacterial promoter
     CDS             5113..5907
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5971..6019
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      6255..6291
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6962..7247
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggcgaccaag aaggcaggct
     1621 cgcggctgga gacggagatc gagcgctgcc gctccgagtg ccagtgggag cggatccctg
     1681 agctcgtcaa gcagctgtcg gccaagctca tcgccaacga tgacatggca gagcttctcc
     1741 tcggggagtc gaagctggag cagtacctga aggaacaccc cctgaggcag ggggccagtc
     1801 cccgaggccc caagccccag ctgactgagg tccgcaagca tctgaccgcc gccctggacc
     1861 gagggaacct taagtcagaa ttcctacaag aatccaatct gatcatggcc aagttgaatt
     1921 atgtggaagg tgattataaa gaagctctga acatttacgc ccgggtgggc ctggacgatc
     1981 tgccactgac agctgtcccg ccctacaggc tgcgggtgat cgcagaagcc tacgctacca
     2041 aaggactttg tttggagaag ctgcctattt cttcttctac cagtaatctc catgtggacc
     2101 gggaacagga tgtcatcacc tgttatgaga aagcagggga catcgcactc ctgtatctcc
     2161 aagagataga aagggtaata ctttctaata ttcaaaacag aagccctaag cctggccctg
     2221 ctccccacga tcaagaacta ggttttttcc tagaaacagg acttcagaga gcccatgtcc
     2281 tctatttcaa aaatgggaac ttgacaagag gagtcggaag atttagagag cttctcagag
     2341 cagttgaaac aagaacgact caaaacctgc gaatgacaat agccaggcag ctggcagaga
     2401 tcttgttgcg gggtatgtgt gagcagagct actggaaccc tctggaggat ccaccgtgcc
     2461 agtcacctct ggacgatcct ctccgcaaag gagcaaacac aaaaacctac actctcactc
     2521 ggagagcccg tgtctactca ggagagaaca ttttttgtcc tcaagaaaat acggaagaag
     2581 ccctgttgtt attgctgatt agtgaatcaa tggccaaccg ggacgctgtg ctgagcagga
     2641 tacctgaaca caagagtgac cgcctcatca gtctgcagag tgcatctgtg gtctatgact
     2701 tactcaccat tgctcttgga agaagaggcc agtatgagat gctgtcagag tgcctagaaa
     2761 gagccatgaa gtttgccttt gaggaattcc acctgtggta ccagtttgct ctgtccctga
     2821 tggctgctgg aaaatctgcc cgtgccgtga aggtgctgaa agagtgtatc cgcctgaagc
     2881 cagacgatgc caccatccct ctcctcgctg ccaagctctg catgggctcc ctgcactggt
     2941 tggaagaggc tgaaaagttt gccaaaactg tcgttgatgt gggagagaaa acgtcagagt
     3001 tcaaggccaa aggctactta gctctggggc tcacgtacag tctgcaggcc actgacgctt
     3061 ctttgcgagg gatgcaggag gtcctacaga gaaaggcgct tcttgcattt cagagggccc
     3121 acagcctgtc acccacagat caccaagcag ctttctacct ggctctgcag cttgccatct
     3181 ccagacagat cccagaggct ctggggtatg tccgccaagc tcttcagctt caaggtgacg
     3241 atgccaactc cctgcacctc cttgccctcc tgctgtcagc acagaagcat taccatgacg
     3301 ctctgaacat catcgacatg gccctgagtg aatacccaga aaatttcata ctactgtttt
     3361 ccaaagtgaa gttgcagtca ctctgccgag gcccggacga ggcactgctg acttgtaagc
     3421 acatgctgca gatatggaaa tcctgctaca acctcaccaa ccccagtgat tctggacgtg
     3481 ggagcagcct cttagataga accattgctg acagacgaca gcttaataca attactttgc
     3541 cagacttcag cgatcccgag acaggctccg tccatgccac atcggtagca gcctcaagag
     3601 tggagcaggc actgtcggaa gtggcttcgt ctctgcagag cagtgcccct aagcagggcc
     3661 cgctgcaccc ctggatgacg ctggcacaga tctggctcca tgcagctgaa gtctatatcg
     3721 gcatcgggaa gcctgcagaa gccacagcct gtacccaaga agctgccaac ctcttcccaa
     3781 tgtcccacaa tgtcctctac atgcgcggcc agattgctga gctccgggga agcatggacg
     3841 aggcgcggcg gtggtatgaa gaggccttag ccatcagccc cacccacgtg aagagcatgc
     3901 agcgactggc cctgatcctt caccagctag gccgctacag tctggcggag aagatcctcc
     3961 gggacgcggt gcaggtgaac tcgacagccc acgaggtctg gaacgggctg ggcgaggtcc
     4021 tccaagctca gggcaacgat gcggcggcta cggagtgctt cctgacagcc ttggagctgg
     4081 aggccagcag ccccgccgtg cccttcacca tcatcccccg cgtgctcgtt taaacgaatt
     4141 cgggctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc tgatccggct
     4201 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     4261 taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     4321 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     4381 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     4441 tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa
     4501 atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     4561 tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg
     4621 cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag
     4681 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4741 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4801 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4861 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4921 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4981 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaatta actgttgaca
     5041 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaaccc
     5101 aggaggcaga tcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5161 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5221 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5281 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5341 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5401 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5461 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5521 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5581 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5641 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5701 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5761 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5821 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5881 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5941 cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg
     6001 tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc tctttgcgct tgcgttttcc
     6061 cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg
     6121 gctttctacc cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6661 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     6721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6841 tttcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttatag
     6961 tccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa
     7021 atggcagctt cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt
     7081 attcatacgg ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg gtttgttgcc
     7141 atttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa
     7201 gggattatgg taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagtac
     7261 tccggccacg atgcgtccgg cgtagaggat cgagatct
//
