LOCUS       Exported                7683 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..2169
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     polyA_signal    52..273
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(297..330)
                     /label=Cre site
                     /note="Cre site"
     promoter        339..405
                     /label=EM7 bacterial promoter
     CDS             419..1213
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    1220..1257
                     /label=Untitled
     rep_origin      1368..1404
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(2086..2133)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(2135..2169)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    2145..2998
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    2145..2179
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(2157..2555)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(2580..2998)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(2973..2998)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    2990..2998
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    2999..3265
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    2999
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(3020..3207)
                     /label=SV40\polyA\signal
     misc_feature    complement(3211..3267)
                     /label=MCS 2
     misc_feature    complement(3268..3273)
                     /label=MCS 2
     misc_feature    3268..3273
                     /label=AscI
                     /note="AscI"
     promoter        3274..3864
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          3979..4111
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        4155..4174
                     /label=T7 promoter
     misc_feature    4178..4185
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4187..6715
                     /label=TTC7B
                     /note="TTC7B"
     misc_feature    6720..6722
                     /label=EcoICR I
     misc_feature    6722..6766
                     /label=HT7 Linker C terminal
     misc_feature    6767..7657
                     /label=HT7 tag C terminal
     misc_feature    7678..7683
                     /label=SbfI
                     /note="SbfI"
ORIGIN
        1 ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga gcagacatga
       61 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      121 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      181 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
      241 ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagaat tctaatataa
      301 cttcgtatag catacattat acgaagttat ggatccactg ttgacaatta atcatcggca
      361 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
      421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1201 cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2041 tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac
     2101 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagtttag
     2161 ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca
     2221 tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac
     2281 cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg
     2341 ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca
     2401 gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg
     2461 gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg
     2521 agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc agaagaatcg
     2581 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2641 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2701 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2761 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2821 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     2881 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     2941 cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg tgctgcgcaa
     3001 gacgcgttaa ttaagacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     3181 ctacaaatgt ggtatggctg attatgatcc gcggccgcaa tacgtcgacg ttatcagctg
     3241 acttcgtacg agagcctagg attatggcgc gccactagtt attaatagta atcaattacg
     3301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     3361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3481 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     3541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     3601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     3661 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     3781 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3841 gctggtttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa
     3901 attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagaagttgg
     3961 tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag
     4021 aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt
     4081 actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct
     4141 taaggctaga gtattaatac gactcactat agggctagcg atcgccatgg cgaccaagaa
     4201 ggcaggctcg cggctggaga cggagatcga gcgctgccgc tccgagtgcc agtgggagcg
     4261 gatccctgag ctcgtcaagc agctgtcggc caagctcatc gccaacgatg acatggcaga
     4321 gcttctcctc ggggagtcga agctggagca gtacctgaag gaacaccccc tgaggcaggg
     4381 ggccagtccc cgaggcccca agccccagct gactgaggtc cgcaagcatc tgaccgccgc
     4441 cctggaccga gggaacctta agtcagaatt cctacaagaa tccaatctga tcatggccaa
     4501 gttgaattat gtggaaggtg attataaaga agctctgaac atttacgccc gggtgggcct
     4561 ggacgatctg ccactgacag ctgtcccgcc ctacaggctg cgggtgatcg cagaagccta
     4621 cgctaccaaa ggactttgtt tggagaagct gcctatttct tcttctacca gtaatctcca
     4681 tgtggaccgg gaacaggatg tcatcacctg ttatgagaaa gcaggggaca tcgcactcct
     4741 gtatctccaa gagatagaaa gggtaatact ttctaatatt caaaacagaa gccctaagcc
     4801 tggccctgct ccccacgatc aagaactagg ttttttccta gaaacaggac ttcagagagc
     4861 ccatgtcctc tatttcaaaa atgggaactt gacaagagga gtcggaagat ttagagagct
     4921 tctcagagca gttgaaacaa gaacgactca aaacctgcga atgacaatag ccaggcagct
     4981 ggcagagatc ttgttgcggg gtatgtgtga gcagagctac tggaaccctc tggaggatcc
     5041 accgtgccag tcacctctgg acgatcctct ccgcaaagga gcaaacacaa aaacctacac
     5101 tctcactcgg agagcccgtg tctactcagg agagaacatt ttttgtcctc aagaaaatac
     5161 ggaagaagcc ctgttgttat tgctgattag tgaatcaatg gccaaccggg acgctgtgct
     5221 gagcaggata cctgaacaca agagtgaccg cctcatcagt ctgcagagtg catctgtggt
     5281 ctatgactta ctcaccattg ctcttggaag aagaggccag tatgagatgc tgtcagagtg
     5341 cctagaaaga gccatgaagt ttgcctttga ggaattccac ctgtggtacc agtttgctct
     5401 gtccctgatg gctgctggaa aatctgcccg tgccgtgaag gtgctgaaag agtgtatccg
     5461 cctgaagcca gacgatgcca ccatccctct cctcgctgcc aagctctgca tgggctccct
     5521 gcactggttg gaagaggctg aaaagtttgc caaaactgtc gttgatgtgg gagagaaaac
     5581 gtcagagttc aaggccaaag gctacttagc tctggggctc acgtacagtc tgcaggccac
     5641 tgacgcttct ttgcgaggga tgcaggaggt cctacagaga aaggcgcttc ttgcatttca
     5701 gagggcccac agcctgtcac ccacagatca ccaagcagct ttctacctgg ctctgcagct
     5761 tgccatctcc agacagatcc cagaggctct ggggtatgtc cgccaagctc ttcagcttca
     5821 aggtgacgat gccaactccc tgcacctcct tgccctcctg ctgtcagcac agaagcatta
     5881 ccatgacgct ctgaacatca tcgacatggc cctgagtgaa tacccagaaa atttcatact
     5941 actgttttcc aaagtgaagt tgcagtcact ctgccgaggc ccggacgagg cactgctgac
     6001 ttgtaagcac atgctgcaga tatggaaatc ctgctacaac ctcaccaacc ccagtgattc
     6061 tggacgtggg agcagcctct tagatagaac cattgctgac agacgacagc ttaatacaat
     6121 tactttgcca gacttcagcg atcccgagac aggctccgtc catgccacat cggtagcagc
     6181 ctcaagagtg gagcaggcac tgtcggaagt ggcttcgtct ctgcagagca gtgcccctaa
     6241 gcagggcccg ctgcacccct ggatgacgct ggcacagatc tggctccatg cagctgaagt
     6301 ctatatcggc atcgggaagc ctgcagaagc cacagcctgt acccaagaag ctgccaacct
     6361 cttcccaatg tcccacaatg tcctctacat gcgcggccag attgctgagc tccggggaag
     6421 catggacgag gcgcggcggt ggtatgaaga ggccttagcc atcagcccca cccacgtgaa
     6481 gagcatgcag cgactggccc tgatccttca ccagctaggc cgctacagtc tggcggagaa
     6541 gatcctccgg gacgcggtgc aggtgaactc gacagcccac gaggtctgga acgggctggg
     6601 cgaggtcctc caagctcagg gcaacgatgc ggcggctacg gagtgcttcc tgacagcctt
     6661 ggagctggag gccagcagcc ccgccgtgcc cttcaccatc atcccccgcg tgctcgtttc
     6721 tctcgagcca accactgagg atctgtactt tcagagcgat aacgatggat ccgaaatcgg
     6781 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     6841 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     6901 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     6961 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     7021 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     7081 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     7141 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     7201 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     7261 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     7321 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     7381 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     7441 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     7501 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     7561 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     7621 gatcgcgcgc tggctgtcta ctctggagat ttccggttaa tagaattcta gagtcgacct
     7681 gca
//
