LOCUS       Exported                6380 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural protein sequence
  ORGANISM  unspecified
REFERENCE   1  (residues 1 to 6380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)      5128 bp    DNA   circular         
              9/3/2015

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) Old/Alternate 
            Name(s):pF-Nn5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="unspecified"
     region          1..742
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
                     /label=CMV immediate-early enhancer/promoter
     region          857..989
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
                     /label=chimeric intron
     region          1033..1052
                     /label=T7 RNA Polymerase promoter
     region          1065..1577
                     /note="N Terminal version. Renamed from 'N terminal 
                     NanoLuc' Feb 2015"
                     /label=NanoLuc
     region          1068..1577
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
                     /label=NanoLuc
     region          1068..1577
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
                     /label=NanoLuc
     region          1578..1589
                     /label=N Terminal NanoLuc linker
     region          1590..1597
                     /note="Used in Flexi Vector Cloning"
                     /label=SgfI Site
     region          1599..3209
                     /note="FYN"
                     /label=FYN
     region          3210..3217
                     /note="Used in Flexi Vector Cloning"
                     /label=PmeI site
     region          3214..3347
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
                     /label=Transfer Efficiency Cassette
     region          3369..3590
                     /label=Sv40 late polyadenylation signal
     region          3689..4107
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
                     /label=SV40 enhancer and early promoter
     region          3762..3998
                     /note=" Feature was found in the reverse orientation."
                     /label=SV40 Enhancer
     region          4005..4070
                     /label=Sv40 Min Ori
     region          4115..4181
                     /label=EM7 bacterial promoter
     region          4195..4989
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
                     /label=Neomycin phosphotransferase coding
     region          5053..5101
                     /label=Synthetic PolyAdenylation Signal
     region          5337..5373
                     /note="The actual origin is the first base."
                     /label=ColE1-derived plasmid replication origin
     region          6044..6329
                     /note="Prevents multimerization"
                     /label=Cer Region
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gggctgtgtg caatgtaagg
     1621 ataaagaagc aacaaaactg acggaggaga gggacggcag cctgaaccag agctctgggt
     1681 accgctatgg cacagacccc acccctcagc actaccccag cttcggtgtg acctccatcc
     1741 ccaactacaa caacttccac gcagccgggg gccaaggact caccgtcttt ggaggtgtga
     1801 actcttcgtc tcatacgggg accttgcgta cgagaggagg aacaggagtg acactctttg
     1861 tggcccttta tgactatgaa gcacggacag aagatgacct gagttttcac aaaggagaaa
     1921 aatttcaaat attgaacagc tcggaaggag attggtggga agcccgctcc ttgacaactg
     1981 gagagacagg ttacattccc agcaattatg tggctccagt tgactctatc caggcagaag
     2041 agtggtactt tggaaaactt ggccgaaaag atgctgagcg acagctattg tcctttggaa
     2101 acccaagagg tacctttctt atccgcgaga gtgaaaccac caaaggtgcc tattcacttt
     2161 ctatccgtga ttgggatgat atgaaaggag accatgtcaa acattataaa attcgcaaac
     2221 ttgacaatgg tggatactac attaccaccc gggcccagtt tgaaacactt cagcagcttg
     2281 tacaacatta ctcagagaga gctgcaggtc tctgctgccg cctagtagtt ccctgtcaca
     2341 aagggatgcc aaggcttacc gatctgtctg tcaaaaccaa agatgtctgg gaaatccctc
     2401 gagaatccct gcagttgatc aagagactgg gaaatgggca gtttggggaa gtatggatgg
     2461 gtacctggaa tggaaacaca aaagtagcca taaagactct taaaccaggc acaatgtccc
     2521 ccgaatcatt ccttgaggaa gcgcagatca tgaagaagct gaagcacgac aagctggtcc
     2581 agctctatgc agtggtgtct gaggagccca tctacatcgt caccgagtat atgaacaaag
     2641 gaagtttact ggatttctta aaagatggag aaggaagagc tctgaaatta ccaaatcttg
     2701 tggacatggc agcacaggtg gctgcaggaa tggcttacat cgagcgcatg aattatatcc
     2761 atagagatct gcgatcagca aacattctag tggggaatgg actcatatgc aagattgctg
     2821 acttcggatt ggcccgattg atagaagaca atgagtacac agcaagacaa ggtgcaaagt
     2881 tccccatcaa gtggacggcc cccgaggcag ccctgtacgg gaggttcaca atcaagtctg
     2941 acgtgtggtc ttttggaatc ttactcacag agctggtcac caaaggaaga gtgccatacc
     3001 caggcatgaa caaccgggag gtgctggagc aggtggagcg aggctacagg atgccctgcc
     3061 cgcaggactg ccccatctct ctgcatgagc tcatgatcca ctgctggaaa aaggaccctg
     3121 aagaacgccc cacttttgag tacttgcaga gcttcctgga agactacttt accgcgacag
     3181 agccccagta ccaacctggt gaaaacctgg tttaaacgaa ttcgggctcg gtacccgggg
     3241 atcctctaga gtcgacctgc aggcatgcaa gctgatccgg ctgctaacaa agcccgaaag
     3301 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcggcc
     3361 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3421 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3481 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3541 ggagatgtgg gaggtttttt taagcaagta aaacctctac aaatgtggta aaatcgaatt
     3601 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     3661 cggtatttca caccgcatac gcggatctgc gcagcaccat ggcctgaaat aacctctgaa
     3721 agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     3781 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3841 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3901 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3961 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4021 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4081 ggaggcctag gcttttgcaa aaagcttaat taactgttga caattaatca tcggcatagt
     4141 atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca gatcatgatt
     4201 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4261 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4321 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     4381 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4441 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4501 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4561 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4621 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4681 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     4741 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4801 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4861 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4921 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4981 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5041 cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     5101 gaatcgatag cgataaggat cctctttgcg cttgcgtttt cccttgtcca gatagcccag
     5161 tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta cccggtatca
     5221 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5281 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5761 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatttcaaga agatcctttg
     5941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6001 atgagattat caaaaaggat cttcacctag atccttttat agtccggaaa tacaggaacg
     6061 cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc ttcagtggat
     6121 taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac ggttaaaatt
     6181 tatcaggcgc gattgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     6241 ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca
     6301 cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca cgatgcgtcc
     6361 ggcgtagagg atcgagatct
//
