LOCUS       Exported                5667 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..2169
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     polyA_signal    52..273
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(297..330)
                     /label=Cre site
                     /note="Cre site"
     promoter        339..405
                     /label=EM7 bacterial promoter
     CDS             419..1213
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    1220..1257
                     /label=Untitled
     rep_origin      1368..1404
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(2086..2133)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(2135..2169)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    2145..2998
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    2145..2179
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(2157..2555)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(2580..2998)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(2973..2998)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    2990..2998
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    2999..3265
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    2999
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(3020..3207)
                     /label=SV40\polyA\signal
     misc_feature    complement(3211..3267)
                     /label=MCS 2
     misc_feature    complement(3268..3273)
                     /label=MCS 2
     misc_feature    3268..3273
                     /label=AscI
                     /note="AscI"
     promoter        3274..3864
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          3979..4111
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        4155..4174
                     /label=T7 promoter
     misc_feature    4178..4185
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4187..4699
                     /label=MBP
                     /note="MBP"
     misc_feature    4706..4750
                     /label=HT7 Linker C terminal
     misc_feature    4751..5641
                     /label=HT7 tag C terminal
     misc_feature    5662..5667
                     /label=SbfI
                     /note="SbfI"
ORIGIN
        1 ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga gcagacatga
       61 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      121 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      181 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
      241 ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagaat tctaatataa
      301 cttcgtatag catacattat acgaagttat ggatccactg ttgacaatta atcatcggca
      361 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
      421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1201 cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2041 tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac
     2101 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagtttag
     2161 ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca
     2221 tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac
     2281 cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg
     2341 ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca
     2401 gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg
     2461 gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg
     2521 agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc agaagaatcg
     2581 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2641 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2701 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2761 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2821 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     2881 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     2941 cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg tgctgcgcaa
     3001 gacgcgttaa ttaagacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     3181 ctacaaatgt ggtatggctg attatgatcc gcggccgcaa tacgtcgacg ttatcagctg
     3241 acttcgtacg agagcctagg attatggcgc gccactagtt attaatagta atcaattacg
     3301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     3361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3481 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     3541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     3601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     3661 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     3781 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3841 gctggtttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa
     3901 attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagaagttgg
     3961 tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag
     4021 aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt
     4081 actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct
     4141 taaggctaga gtattaatac gactcactat agggctagcg atcgccatgg cgtcacagaa
     4201 gagaccctcc cagaggcacg gatccaagta cctggccaca gcaagtacca tggaccatgc
     4261 caggcatggc ttcctcccaa ggcacagaga cacgggcatc cttgactcca tcgggcgctt
     4321 ctttggcggt gacaggggtg cgcccaagcg gggctctggc aaggactcac accacccggc
     4381 aagaactgct cactacggct ccctgcccca gaagtcacac ggccggaccc aagatgaaaa
     4441 ccccgtagtc cacttcttca agaacattgt gacgcctcgc acaccacccc cgtcgcaggg
     4501 aaaggggaga ggactgtccc tgagcagatt tagctggggg gccgaaggcc agagaccagg
     4561 atttggctac ggaggcagag cgtccgacta taaatcggct cacaagggat tcaagggagt
     4621 cgatgcccag ggcacgcttt ccaaaatttt taagctggga ggaagagata gtcgctctgg
     4681 atcacccatg gctagacgcg tttctctcga gccaaccact gaggatctgt actttcagag
     4741 cgataacgat ggatccgaaa tcggtactgg ctttccattc gacccccatt atgtggaagt
     4801 cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt
     4861 cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc
     4921 gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct
     4981 gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct
     5041 ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa
     5101 gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac
     5161 ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt
     5221 cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt
     5281 cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt
     5341 tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa
     5401 catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct
     5461 gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa
     5521 aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga
     5581 caacccggac ctgatcggca gcgagatcgc gcgctggctg tctactctgg agatttccgg
     5641 ttaatagaat tctagagtcg acctgca
//
