LOCUS       Exported                6075 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Previously pFdR4Hc
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..246
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    complement(192..254)
                     /label=MCS 2
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1159..1166
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1168..2088
                     /label=VTA1
                     /note="VTA1"
     misc_feature    2095..2139
                     /label=HT7 Linker C terminal
     misc_feature    2140..3030
                     /label=HaloTag
     misc_feature    3057..5225
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     polyA_signal    3108..3329
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(3353..3386)
                     /label=Cre site
                     /note="Cre site"
     promoter        3395..3461
                     /label=EM7 bacterial promoter
     CDS             3475..4269
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    4276..4313
                     /label=Untitled
     rep_origin      4424..4460
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5142..5189)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(5191..5225)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    5201..6054
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    5201..5235
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(5213..5611)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(5636..6054)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(6029..6054)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    6046..6054
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    6055..6075
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    6055
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc gatcgccatg gccgcgcttg caccgctgcc cccgctcccc
     1201 gcacagttca agagcataca gcatcatctg aggacggctc aggagcatga caagcgagac
     1261 cctgtggtgg cttattactg tcgtttatac gcaatgcaga ctggaatgaa gatcgatagt
     1321 aaaactcctg aatgtcgcaa atttttatca aagttaatgg atcagttaga agctctaaag
     1381 aagcagttgg gtgataatga agctattact caagaaatag tgggctgtgc ccatttggag
     1441 aattatgctt tgaaaatgtt tttgtatgca gacaatgaag atcgtgctgg acgatttcac
     1501 aaaaacatga tcaagtcctt ctatactgca agtcttttga tagatgtcat aacagtattt
     1561 ggagaactca ctgatgaaaa tgtgaaacac aggaagtatg ccagatggaa ggcaacatac
     1621 atccataatt gtttaaagaa tggggagact cctcaagcag gccctgttgg aattgaagaa
     1681 gataatgata ttgaagaaaa tgaagatgct ggagcagcct ctctgcccac tcagccaact
     1741 cagccatcat catcttcaac ttatgaccca agcaacatgc catcaggcaa ctatactgga
     1801 atacagattc ctccgggtgc acacgctcca gctaatacac cagcagaagt gcctcacagc
     1861 acaggtgtag caagtaatac tatccaacct actccacaga ctatacctgc cattgatccc
     1921 gcacttttca atacaatttc ccagggggat gttcgtctaa ccccagaaga ctttgctaga
     1981 gctcagaagt actgcaaata tgctggcagt gctttgcagt atgaagatgt aagcactgct
     2041 gtccagaatc tacaaaaggc tctcaagtta ctgacgacag gcagagaagt ttctctcgag
     2101 ccaaccactg aggatctgta ctttcagagc gataacgatg gatccgaaat cggtactggc
     2161 tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt
     2221 ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg
     2281 tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc
     2341 ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc
     2401 atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg
     2461 ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca
     2521 tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag
     2581 accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt
     2641 tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac
     2701 cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac
     2761 gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac
     2821 tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc
     2881 ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc
     2941 ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg
     3001 cgctggctgt ctactctgga gatttccggt taatagaatt ctagagtcga cctgcaggca
     3061 tgcaagctga tttggctgct gccaccgctg agcaaggccg cttcgagcag acatgataag
     3121 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3181 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3241 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     3301 aagcaagtaa aacctctaca aatgtggtaa aatcttaatt aagaattcta atataacttc
     3361 gtatagcata cattatacga agttatggat ccactgttga caattaatca tcggcatagt
     3421 atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca gatcatgatt
     3481 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     3541 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     3601 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     3661 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     3721 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     3781 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     3841 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     3901 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     3961 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     4021 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4081 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4141 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4201 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4261 ttcttctgat tcgaaatgac cgaccaagcg acgcccaacc ggtatcagct cactcaaagg
     4321 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4381 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4441 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4501 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4561 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4621 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4681 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4741 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4801 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4861 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4921 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4981 agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg
     5041 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5101 aaaggatctt cacctagatc cttttatagt ccggaatcgt cacacaaaaa accaacacac
     5161 agatgtaatg aaaataaaga tattttattg cggccatcgt gatggctaga gtttagccct
     5221 cccacacata accagagggc agcaattcac gaatcccaac tgccgtcggc tgtccatcac
     5281 tgtccttcac tatggctttg atcccaggat gcagatcgag aagcacctgt cggcaccgtc
     5341 cgcaggggct caagatgccc ctgttctcat ttccgatcgc gacgatacaa gtcaggttgc
     5401 cagctgccgc agcagcagca gtgcccagca ccacgagttc tgcacaaggt cccccagtaa
     5461 aatgatatac attgacacca gtgaagatgc ggccgtcgct agagagagct gcgctggcga
     5521 cgctgtagtc ttcagagatg gggatgctgt tgattgtagc cgttgctctt tcaatgaggg
     5581 tggattcttc ttgagacaaa ggcttggcca tggtggcgct agtgtcagaa gaatcgagct
     5641 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     5701 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     5761 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     5821 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     5881 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     5941 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcaga
     6001 aggtacctaa ccaagttcct ctttcagagg ttatttcagg ccatggtgct gcgcaagacg
     6061 cgttaattaa gacgt
//
