LOCUS       Exported                5826 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Previously pFdR4Hc
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..246
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    complement(192..254)
                     /label=MCS 2
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1159..1166
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1168..1839
                     /label=CHMP4B
                     /note="CHMP4B"
     misc_feature    1846..1890
                     /label=HT7 Linker C terminal
     misc_feature    1891..2781
                     /label=HaloTag
     misc_feature    2808..4976
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     polyA_signal    2859..3080
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(3104..3137)
                     /label=Cre site
                     /note="Cre site"
     promoter        3146..3212
                     /label=EM7 bacterial promoter
     CDS             3226..4020
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    4027..4064
                     /label=Untitled
     rep_origin      4175..4211
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4893..4940)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(4942..4976)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    4952..5805
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    4952..4986
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(4964..5362)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(5387..5805)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(5780..5805)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    5797..5805
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    5806..5826
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    5806
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc gatcgccatg tcggtgttcg ggaagctgtt cggggctgga
     1201 gggggtaagg ccggcaaggg cggcccgacc ccccaggagg ccatccagcg gctgcgggac
     1261 acggaagaga tgttaagcaa gaaacaggag ttcctggaga agaaaatcga gcaggagctg
     1321 acggccgcca agaagcacgg caccaaaaac aagcgcgcgg ccctccaggc actgaagcgt
     1381 aagaagaggt atgagaagca gctggcgcag atcgacggca cattatcaac catcgagttc
     1441 cagcgggagg ccctggagaa tgccaacacc aacaccgagg tgctcaagaa catgggctat
     1501 gccgccaagg ccatgaaggc ggcccatgac aacatggaca tcgataaagt tgatgagtta
     1561 atgcaggaca ttgctgacca gcaagaactt gcagaggaga tttcaacagc aatttcgaaa
     1621 cctgtagggt ttggagaaga gtttgacgag gatgagctca tggcggaatt agaagaacta
     1681 gaacaggagg aactagacaa gaatttgctg gaaatcagtg gacccgaaac agtccctcta
     1741 ccaaatgttc cctctatagc cctaccatca aaacccgcca agaagaaaga agaggaggac
     1801 gacgacatga aggaattgga gaactgggct ggatccatgg tttctctcga gccaaccact
     1861 gaggatctgt actttcagag cgataacgat ggatccgaaa tcggtactgg ctttccattc
     1921 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
     1981 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
     2041 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
     2101 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
     2161 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
     2221 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     2281 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     2341 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     2401 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     2461 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     2521 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     2581 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     2641 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     2701 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     2761 tctactctgg agatttccgg ttaatagaat tctagagtcg acctgcaggc atgcaagctg
     2821 atttggctgc tgccaccgct gagcaaggcc gcttcgagca gacatgataa gatacattga
     2881 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2941 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3001 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
     3061 aaacctctac aaatgtggta aaatcttaat taagaattct aatataactt cgtatagcat
     3121 acattatacg aagttatgga tccactgttg acaattaatc atcggcatag tatatcggca
     3181 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     3241 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3301 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3361 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     3421 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3481 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3541 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3601 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3661 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3721 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     3781 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3841 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3901 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3961 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4021 ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag gcggtaatac
     4081 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4141 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4201 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4261 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4321 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4381 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4441 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4501 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4561 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4621 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4681 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4741 ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg
     4801 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4861 tcacctagat ccttttatag tccggaatcg tcacacaaaa aaccaacaca cagatgtaat
     4921 gaaaataaag atattttatt gcggccatcg tgatggctag agtttagccc tcccacacat
     4981 aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca
     5041 ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc
     5101 tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg
     5161 cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata
     5221 cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt
     5281 cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt
     5341 cttgagacaa aggcttggcc atggtggcgc tagtgtcaga agaatcgagc tttttgcaaa
     5401 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     5461 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     5521 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     5581 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     5641 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     5701 ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag aaggtaccta
     5761 accaagttcc tctttcagag gttatttcag gccatggtgc tgcgcaagac gcgttaatta
     5821 agacgt
//
