LOCUS       Exported                6486 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Previously pFdR4Hc
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..246
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    complement(192..254)
                     /label=MCS 2
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1159..1166
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1168..2499
                     /label=VPS4B
                     /note="VPS4B"
     misc_feature    2506..2550
                     /label=HT7 Linker C terminal
     misc_feature    2551..3441
                     /label=HaloTag
     misc_feature    3468..5636
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     polyA_signal    3519..3740
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(3764..3797)
                     /label=Cre site
                     /note="Cre site"
     promoter        3806..3872
                     /label=EM7 bacterial promoter
     CDS             3886..4680
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    4687..4724
                     /label=Untitled
     rep_origin      4835..4871
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5553..5600)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(5602..5636)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    5612..6465
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    5612..5646
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(5624..6022)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(6047..6465)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(6440..6465)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    6457..6465
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    6466..6486
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    6466
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc gatcgccatg tcatccactt cgcccaacct ccagaaagcg
     1201 atagatctgg ctagcaaagc agcgcaagaa gacaaggctg ggaactacga agaagccctt
     1261 cagctctatc agcatgctgt gcagtatttt cttcatgtcg ttaaatatga agcacagggt
     1321 gataaagcca agcaaagtat cagggcaaag tgtacagaat atcttgatag agcagaaaaa
     1381 ctaaaggagt acctgaaaaa taaagagaaa aaagcacaga agccagtgaa agaaggacag
     1441 ccgagtccag cagatgagaa ggggaatgac agtgatgggg aaggagaatc tgatgatcct
     1501 gaaaaaaaga aactacagaa tcaacttcaa ggtgccattg ttatagaacg accaaatgtg
     1561 aaatggagtg acgttgctgg acttgaagga gccaaagaag cactgaaaga ggctgtgata
     1621 ctgcctatta aatttcctca tctttttaca ggcaagagaa caccttggag gggaatccta
     1681 ttatttgggc cgcctggaac aggaaagtcc tacttagcca aagctgtagc aacagaagcc
     1741 aacaactcaa catttttttc aatatcttcc tctgatcttg tttctaagtg gctaggtgaa
     1801 agtgaaaaac tggttaagaa tttattccaa cttgccagag agaacaagcc ctccattatc
     1861 ttcattgatg aaattgattc tctctgtggt tcaagaagtg aaaatgaaag tgaagccgca
     1921 cgtagaatta agacggagtt cctagtgcaa atgcaagggg ttggtgtaga caatgatgga
     1981 attttggttc tgggagctac aaatataccc tgggttctgg attctgccat taggcgaaga
     2041 tttgagaaac gaatttatat tcccttgccg gaaccccatg cccgagcagc aatgtttaaa
     2101 ctgcacctag ggaccactca gaacagtctc acggaagcag actttcggga acttgggagg
     2161 aaaacagatg gttattcagg ggcagatata agtatcattg tacgtgatgc ccttatgcag
     2221 cctgttagga aagtacagtc agctactcat tttaaaaagg ttcgcggacc ttcccgagct
     2281 gatcctaacc atcttgtaga tgatctgcta acaccttgct ctccaggtga ccctggtgcc
     2341 attgaaatga catggatgga tgtccctgga gataaacttt tggagccagt tgtttccatg
     2401 tcggatatgt tgcggtcact atctaacaca aaacctacag tcaatgaaca tgacttgttg
     2461 aaattaaaga agtttacaga agattttggt caagaaggcg tttctctcga gccaaccact
     2521 gaggatctgt actttcagag cgataacgat ggatccgaaa tcggtactgg ctttccattc
     2581 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
     2641 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
     2701 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
     2761 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
     2821 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
     2881 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     2941 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     3001 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     3061 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     3121 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     3181 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     3241 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     3301 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     3361 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     3421 tctactctgg agatttccgg ttaatagaat tctagagtcg acctgcaggc atgcaagctg
     3481 atttggctgc tgccaccgct gagcaaggcc gcttcgagca gacatgataa gatacattga
     3541 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3601 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3661 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
     3721 aaacctctac aaatgtggta aaatcttaat taagaattct aatataactt cgtatagcat
     3781 acattatacg aagttatgga tccactgttg acaattaatc atcggcatag tatatcggca
     3841 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     3901 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3961 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4021 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4081 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4141 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4201 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4261 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4321 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4381 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4441 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4501 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4561 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4621 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4681 ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag gcggtaatac
     4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5401 ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg
     5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5521 tcacctagat ccttttatag tccggaatcg tcacacaaaa aaccaacaca cagatgtaat
     5581 gaaaataaag atattttatt gcggccatcg tgatggctag agtttagccc tcccacacat
     5641 aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca
     5701 ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc
     5761 tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg
     5821 cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata
     5881 cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt
     5941 cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt
     6001 cttgagacaa aggcttggcc atggtggcgc tagtgtcaga agaatcgagc tttttgcaaa
     6061 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     6121 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     6181 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     6241 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     6301 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6361 ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag aaggtaccta
     6421 accaagttcc tctttcagag gttatttcag gccatggtgc tgcgcaagac gcgttaatta
     6481 agacgt
//
