LOCUS       Exported                6103 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..3068
                     /label=MDM4
                     /note="MDM4"
     misc_feature    3069..3076
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3228..3449
                     /label=Sv40 late polyadenylation signal
     promoter        3548..3966
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3974..4040
                     /label=EM7 bacterial promoter
     CDS             4054..4848
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4912..4960
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    5003..5925
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    5180
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gacatcattt tccacctctg
     1621 ctcagtgttc aacatctgac agtgcttgca ggatctctcc tggacaaatc aatcaggtac
     1681 gaccaaaact gccgcttttg aagattttgc atgcagcagg tgcgcaaggt gaaatgttca
     1741 ctgttaaaga ggtcatgcac tatttaggtc agtacataat ggtgaagcaa ctttatgatc
     1801 agcaggagca gcatatggta tattgtggtg gagatctttt gggagaacta ctgggacgtc
     1861 agagcttctc cgtgaaagac ccaagccctc tctatgatat gctaagaaag aatcttgtca
     1921 ctttagccac tgctactaca gatgctgctc agactctcgc tctcgcacag gatcacagta
     1981 tggatattcc aagtcaagac caactgaagc aaagtgcaga ggaaagttcc acttccagaa
     2041 aaagaactac agaagacgat atccccacac tgcctacctc agagcataaa tgcatacatt
     2101 ctagagaaga tgaagactta attgaaaatt tagcccaaga tgaaacatct aggctggacc
     2161 ttggatttga ggagtgggat gtagctggcc tgccttggtg gtttttagga aacttgagaa
     2221 gcaactatac acctagaagt aatggctcaa ctgatttaca gacaaatcag gatgtgggta
     2281 ctgccattgt ttcagatact acagatgact tgtggttttt gaatgagtca gtatcagagc
     2341 agttaggtgt tggaataaaa gttgaagctg ctgatactga acaaacaagt gaagaagtag
     2401 ggaaagtaag tgacaaaaag gtgattgaag tgggaaaaaa tgatgacctg gaggactcta
     2461 agtccttaag tgatgatacc gatgtagagg ttacctctga ggatgagtgg cagtgtactg
     2521 aatgcaagaa atttaactct ccaagcaaga ggtactgttt tcgttgttgg gccttgagga
     2581 aggattggta ttcagattgt tcaaagttaa cccattctct ctccacgtct gatatcactg
     2641 ccatacctga aaaggaaaat gaaggaaatg atgtccctga ttgtcgaaga accatttcgg
     2701 ctcctgtcgt tagacctaaa gatgcgtata taaagaaaga aaactccaaa ctttttgatc
     2761 cctgcaactc agtggaattc ttggatttgg ctcacagttc tgaaagccaa gagaccatct
     2821 caagcatggg agaacagtta gataaccttt ctgaacagag aacagataca gaaaacatgg
     2881 aggattgcca gaatctcttg aagccatgta gcttatgtga gaaaagacca cgagacggga
     2941 acattattca tggaaggacg ggccatcttg tcacttgttt tcactgtgcc agaagactaa
     3001 agaaggctgg ggcttcatgc cctatttgca agaaagagat tcagctggtt attaaggttt
     3061 ttatagcagt ttaaacgaat tcgggctcgg tacccgggga tcctctagag tcgacctgca
     3121 ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     3181 accgctgagc aataactagc ataacccctt ggggcggccg cttcgagcag acatgataag
     3241 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3301 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3361 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     3421 aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata ttaacgctta
     3481 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     3541 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     3601 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3721 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3781 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3841 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3901 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3961 aagcttaatt aactgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     4021 acaaggtgag gaactaaacc caggaggcag atcatgattg aacaagatgg attgcacgca
     4081 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4141 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4201 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     4261 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4321 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4381 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4441 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4501 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4561 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     4621 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4681 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4741 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4801 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4861 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata
     4921 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc
     4981 cgctcatgcg atgataagct gtcaaacatg agaattacaa cttatatcgt atggggctga
     5041 cttcaggtgc tacatttgaa gagataaatt gcactgaaat ctagaaatat tttatctgat
     5101 taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg
     5161 aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc
     5221 gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac
     5281 cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt
     5341 gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5401 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     5461 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
     5521 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
     5581 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
     5641 aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt
     5701 atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc
     5761 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac
     5821 atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc acttcactga
     5881 caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct gatgtccggc
     5941 ggtgcttttg ccgttacgca ccaccccgtc atgagattac gcgttttagc ggtgcgtaca
     6001 attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
     6061 agtactccgg ccacgatgcg tccggcgtag aggatcgaga tct
//
