LOCUS       Exported                6848 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    809..2113
                     /label=TEAD3
                     /note="TEAD3"
     misc_feature    complement(2115..2122)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2127..2153
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2154..2170)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2173..2218)
                     /label=Minimal CMV Promoter 2
     misc_feature    2196..2218
                     /label=core
                     /note="core"
     promoter        2228..2782
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2800..2866
                     /label=EM7 bacterial promoter
     CDS             2880..3539
                     /label=Chloramphenicol resistance
     rep_origin      3708..3744
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4426..4473)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4497..5291)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5305..5599)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5670..6848)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacgt ctttgacgag cttgtagaca tggtgctggg
      841 ccccgtgctc actggtggag acttcgaaga caaaagcaat gacaagcagg gtctcctggg
      901 agtcccggct cgtgaccacc tgcaggatgg tgaagttctc cagcacgctg ttcatcatgt
      961 acttctcggg caggtgcttc agcttgtgga tgaagttgat catgtactcg cacatgggcg
     1021 agcggtggat acggtacaca aagcgcccgt tctccagcct ggcatactca gtctccacct
     1081 tctctaccac ctgtttgcca aaggagcaca ccttggtgga gacgctgatg gtcatgctat
     1141 cagcagagct gtactgagag ctgaccccat agaaggctcc cgggccctcc tggatggtgc
     1201 tgttgaggtc ggcccagaac ttgacaagga agaaggcatt agggggcccc ttctcataga
     1261 gctccttcaa tcctcccttt ttctcgggga atttgtcata gatctggcgc acatctactg
     1321 cctccagggg tgggtctgag aaggcggggt tcgtctggcc gatgtgcaca aacaggtgtt
     1381 tgctgtacgt gtcagggtct cgctgcacct ccatgaaggc tgaatactcc aggagccgca
     1441 gccgggagga ggcaatggta cggtcctgcc acacaggcac agaggcagca gctgagggga
     1501 gcggggccag gggctcataa ctgctgagcg tcggcggcag gggcggctgg atggggtagg
     1561 ctggctgtgc aaagggcttg atgtcctgag agggtccagg ctgctgtccc aggagagggg
     1621 ggctgctcca gaaccgcgag gaagtggaga agacggcctg gggcagaggg gaaggtgggc
     1681 tgaacttgtt ctgcaggaca ctggcagaga cgatctgggc agaggacatg gacgccatgc
     1741 tctgaagggc tttgtccttg gagacctggt ccaggttcat ggccttgatg ccaacctggt
     1801 actcccgcac cttcttccga gctagaacct gtatgtggct ggacacctgt tttctcgtcc
     1861 gagtcttccc cgtcctcagt ttaatatagc gtgcaatcaa ctcatttcgg ccgtacatct
     1921 tgccctcgtc tgacaggatg atcttccgcc ggccgcaggg cgggtagatg gccagggcct
     1981 cctggaagct ctgctcgatg tccgggctcc acacgccctc cgcatcgttg tccagcccct
     2041 tgtccaggcc ctcgggccca tcctcccggg cctccccggg gctgctgctg gcgttccagc
     2101 tgttggacgc catggcgatc gctagctgca aaaagaacaa gtagcttgta ttctatagtg
     2161 tcacctaaat gggcgatctg acggttcact aaaccagctc tgcttatata ggcctccctg
     2221 gcgcgccact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     2281 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2341 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2401 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2461 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2521 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2581 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2641 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2701 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2761 taggcgtgta cggtgggagg tcaaatcgaa ttctaatact gttgacaatt aatcatcggc
     2821 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca
     2881 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2941 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     3001 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     3061 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     3121 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     3181 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     3241 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     3301 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     3361 ccaatatgga caacttcttc gcccccgttt tcactatggg caaatattat acgcaaggcg
     3421 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
     3481 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaag
     3541 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     3601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4141 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4261 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     4321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4381 tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac
     4441 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagttcag
     4501 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     4561 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     4621 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     4681 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccgtg ggtcacgacg
     4741 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     4801 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4861 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4921 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4981 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     5041 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     5101 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     5161 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     5221 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     5281 tgttcaatca tggtggcatc gattcccggt caacatccag tacacctccg gcaccaccgg
     5341 cttccccaag ggcgccacgc tctcccacca caacatcctc aacaacggct actgggtggg
     5401 ccgcacggtc ggctacacgg agcaggaccg ggtgtgtctg ccggtgccct tctaccactg
     5461 cttcggcatg gtcatgggca acttgggcgc cacctcccac gccgcctgca tcgtcatccc
     5521 ggccccgtcc ttcgagccgg cggcgacgct ggaggcggtg cagcgggaga ggtgcacgtc
     5581 cctgtacggc gtccccacga agccgaattc caccacactg gactagatgc atgctcgaca
     5641 gtgtgatgga tatctgcaga attcggcttt cacgacacct gaaatggaag aaaaaaactt
     5701 tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc
     5761 aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg
     5821 ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc
     5881 gagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg
     5941 gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag
     6001 cgccgcgtcc tccattttga gctgcctgca gcagggccgg gaagcggcca tctttccgct
     6061 cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc
     6121 gcgaggccag gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt
     6181 ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg
     6241 ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc atttacaaga
     6301 ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat ggctagagac
     6361 ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag
     6421 cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc
     6481 gaactctccc acccacttcc aacccgaagc tcgggatcaa gaatcacgta ctgcagccag
     6541 gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa agaggccagg cccgcgggaa
     6601 ccacacacgg cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg
     6661 gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg gagccagtac
     6721 acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt tcaattgccg
     6781 acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca ctgacgggca
     6841 ccggagcc
//
