LOCUS       Exported                6884 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    809..2149
                     /label=TEAD2
                     /note="TEAD2"
     misc_feature    complement(2151..2158)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2163..2189
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2190..2206)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2209..2254)
                     /label=Minimal CMV Promoter 2
     misc_feature    2232..2254
                     /label=core
                     /note="core"
     promoter        2264..2818
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2836..2902
                     /label=EM7 bacterial promoter
     CDS             2916..3575
                     /label=Chloramphenicol resistance
     rep_origin      3744..3780
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4462..4509)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4533..5327)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5341..5635)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5706..6884)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacgt ccctgaccag gcggtaaatg tgatgctggg
      841 ccccacgctc gctggtggag acctcgaaga cataggcggt gcagagcagc agttcctggg
      901 tgtctctgtt tgtcaccacc tggaggatgg tgaagttttc caggacgctg ttcatcatgt
      961 atcgctcagg cagctgccgc aacttgtgca agaaattcac caggtactcg cacatgggcg
     1021 agcgcagcag gcggtacaca aatctgccgt cctccagctg ggcccgttcc gtctccacct
     1081 tctccaccac ctgcttgcca aaagagcaga ccttggagga acaggtgagg gtcatgtgtt
     1141 ccaggctctc atactggctg ctcactccgt agaagccacc actgctgatg ctgccaccgg
     1201 cccctgcctc ctcaccactt gggccccagt tcaggtccgc ccagaacttg accaggaaga
     1261 aggcatgggg ggggccacga tcatatagct ctcggaggcc accctttttc tcagggaatt
     1321 tgtcgtagat ctgccggacg tccacactct cgagcggcgg cgctccgggg ctggggcagt
     1381 gctggctgat gtgcacgaac aggtgcctct ggtaagaatc aactgcatct ggcggttcca
     1441 cgaaggctga gaactctacc agctgcaacc gggcggtgcc caggccccga gcctgccagg
     1501 ctgggggcga tggggtaggt gggggcaggg gtgagagggc ttgggggggc tcgtaccctg
     1561 ggaggtcagt agatggggga gtcagtgaca aggtgaacgg tgtctgtgag aatggcttca
     1621 catctggaac attccagggg ggcccagatc ctccagacca aaactggaaa agctcagagg
     1681 cctgaggacc agtgggaccc agtttggcct gcagagaagg cgcggagatg agctgggcag
     1741 aggacatggt tgccattgtc tggaaagcct tgtccttgga aacctggtcc ttcaacttgg
     1801 actggatttc ccttgatttc cttcgggcca aaacctggat gtgactagaa acctgttttc
     1861 gagttcgggt cttccccgtt ctcagcttga tgtagcgggc gatcagttca ttccgaccat
     1921 acatcttgcc ttcatcagac aaaattattt tccggcggcc gcagggtgga tagatggcca
     1981 gggcctcctg gaagctctgc tcaatgtctg ggctccacac cccctctgca tccgggcccc
     2041 cgtcaccccc agccccctca ctgccgccgg taccctcctc actgccttcc tcactgcccg
     2101 tccagccgct gccatcgtcc agggcggccc cagcccgggg ttcccccatg gcgatcgcta
     2161 gctgcaaaaa gaacaagtag cttgtattct atagtgtcac ctaaatgggc gatctgacgg
     2221 ttcactaaac cagctctgct tatataggcc tccctggcgc gccactagtt attaatagta
     2281 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2341 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2401 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2461 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
     2521 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
     2581 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2641 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2701 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2761 tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcaa
     2821 atcgaattct aatactgttg acaattaatc atcggcatag tatatcggca tagtataata
     2881 cgacaaggtg aggaactaaa cccaggaggc agatcatgga gaaaaaaatc actggatata
     2941 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     3001 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     3061 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     3121 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     3181 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     3241 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     3301 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     3361 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     3421 ccgttttcac tatgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     3481 ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     3541 aacagtactg cgatgagtgg cagggcgggg cgtaagcggg actctggggt tcgaaatgac
     3601 cgaccaagcg acgcccaacc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4321 aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4441 cttttatagt ccggaatcgt cacacaaaaa accaacacac agatgtaatg aaaataaaga
     4501 tattttattg cggccatcgt gatggctaga gttcagaaga actcgtcaag aaggcgatag
     4561 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4621 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     4681 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     4741 atattcggca agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatgcgc
     4801 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4861 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4921 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4981 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     5041 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     5101 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
     5161 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5221 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     5281 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatggt ggcatcgatt
     5341 cccggtcaac atccagtaca cctccggcac caccggcttc cccaagggcg ccacgctctc
     5401 ccaccacaac atcctcaaca acggctactg ggtgggccgc acggtcggct acacggagca
     5461 ggaccgggtg tgtctgccgg tgcccttcta ccactgcttc ggcatggtca tgggcaactt
     5521 gggcgccacc tcccacgccg cctgcatcgt catcccggcc ccgtccttcg agccggcggc
     5581 gacgctggag gcggtgcagc gggagaggtg cacgtccctg tacggcgtcc ccacgaagcc
     5641 gaattccacc acactggact agatgcatgc tcgacagtgt gatggatatc tgcagaattc
     5701 ggctttcacg acacctgaaa tggaagaaaa aaactttgaa ccactgtctg aggcttgaga
     5761 atgaaccaag atccaaactc aaaaagggca aattccaagg agaattacat caagtgccaa
     5821 gctggcctaa cttcagtctc cacccactca gtgtggggaa actccatcgc ataaaacccc
     5881 tccccccaac ctaaagacga cgtactccaa aagctcgaga actaatcgag gtgcctggac
     5941 ggcgcccggt actccgtgga gtcacatgaa gcgacggctg aggacggaaa ggcccttttc
     6001 ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc gcgtcctcca ttttgagctg
     6061 cctgcagcag ggccgggaag cggccatctt tccgctcacg caactggtgc cgaccgggcc
     6121 agccttgccg cccagggcgg ggcgatacac ggcggcgcga ggccaggcac cagagcaggc
     6181 cggccagctt gagactaccc ccgtccgatt ctcggtggcc gcgctcgcag gccccgcctc
     6241 gccgaacatg tgcgctggga cgcacgggcc ccgtcgccgc ccgcggcccc aaaaaccgaa
     6301 ataccagtgt gcagatcttg gcccgcattt acaagactat cttgccagaa aaaaagcgtc
     6361 gcagcaggtc atcaaaaatt ttaaatggct agagacttat cgaaagcagc gagacaggcg
     6421 cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc caggccaggc ctcaactcaa
     6481 gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac tctcccaccc acttccaacc
     6541 cgaagctcgg gatcaagaat cacgtactgc agccaggtgg aagtaattca aggcacgcaa
     6601 gggccataac ccgtaaagag gccaggcccg cgggaaccac acacggcact tacctgtgtt
     6661 ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt
     6721 tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc
     6781 ccgcgccacc ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg
     6841 actgtgggcg atgtgcgctc tgcccactga cgggcaccgg agcc
//
