LOCUS       Exported                7274 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)      5128 bp    DNA   circular         
              9/3/2015

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) Old/Alternate 
            Name(s):pF-Nn5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1599..4103
                     /label=TRIM28_NM_005762
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1577
                     /label=NanoLuc
                     /note="N Terminal version. Renamed from 'N terminal 
                     NanoLuc' Feb 2015"
     CDS             1068..1577
                     /label=NanoLuc
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
     CDS             1068..1577
                     /label=NanoLuc
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..4103
                     /label=TRIM28
                     /note="TRIM28"
     misc_feature    4104..4111
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4108..4241
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    4263..4484
                     /label=Sv40 late polyadenylation signal
     promoter        4583..5001
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(4656..4892)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      4899..4964
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        5009..5075
                     /label=EM7 bacterial promoter
     CDS             5089..5883
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5947..5995
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      6231..6267
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6938..7223
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggcggcctcc gcggcggcag
     1621 cctcggcagc agcggcctcg gccgcctctg gcagcccggg cccgggcgag ggctccgctg
     1681 gcggcgaaaa gcgctccacc gccccttcgg ccgcagcctc ggcctctgcc tcagccgcgg
     1741 cgtcgtcgcc cgcggggggc ggcgccgagg cgctggagct gctggagcac tgcggcgtgt
     1801 gcagagagcg cctgcgaccc gagagggagc cccgcctgct gccctgtttg cactcggcct
     1861 gtagtgcctg cttagggccc gcggcccccg ccgccgccaa cagctcgggg gacggcgggg
     1921 cggcgggcga cggcaccgtg gtggactgtc ccgtgtgcaa gcaacagtgc ttctccaaag
     1981 acatcgtgga gaattatttc atgcgtgata gtggcagcaa ggctgccacc gacgcccagg
     2041 atgcgaacca gtgctgcact agctgtgagg ataatgcccc agccaccagc tactgtgtgg
     2101 agtgctcgga gcctctgtgt gagacctgtg tagaggcgca ccagcgggtg aagtacacca
     2161 aggaccatac tgtgcgctct actgggccag ccaagtctcg ggatggtgaa cgtactgtct
     2221 attgcaacgt acacaagcat gaaccccttg tgctgttttg tgagagctgt gatactctca
     2281 cctgccgaga ctgccagctc aatgcccaca aggaccacca gtaccagttc ttagaggatg
     2341 cagtgaggaa ccagcgcaag ctcctggcct cactggtgaa gcgccttggg gacaaacatg
     2401 caacattgca gaagagcacc aaggaggttc gcagctcaat ccgccaggtg tctgacgtac
     2461 agaagcgtgt gcaagtggat gtcaagatgg ccatcctgca gatcatgaag gagctgaata
     2521 agcggggccg tgtgctggtc aatgatgccc agaaggtgac tgaggggcag caggagcgcc
     2581 tggagcggca gcactggacc atgaccaaga tccagaagca ccaggagcac attctgcgct
     2641 ttgcctcttg ggctctggag agtgacaaca acacagccct tttgctttct aagaagttga
     2701 tctacttcca gctgcaccgg gccctcaaga tgattgtgga tcccgtggag ccacatggcg
     2761 agatgaagtt tcagtgggac ctcaatgcct ggaccaagag tgccgaggcc tttggcaaga
     2821 ttgtggcaga gcgtcctggc actaactcaa caggccctgc acccatggcc cctccaagag
     2881 ccccagggcc cctgagcaag cagggctctg gcagcagcca gcccatggag gtgcaggaag
     2941 gctatggctt tgggtcagga gatgatccct actcaagtgc agagccccat gtgtcaggtg
     3001 tgaaacggtc ccgctcaggt gagggcgagg tgagcggcct tatgcgcaag gtgccacgag
     3061 tgagccttga acgcctggac ctggacctca cagctgacag ccagccaccc gtcttcaagg
     3121 tcttcccagg cagtaccact gaggactaca accttattgt tattgaacgt ggcgctgccg
     3181 ctgcagctac cggccagcca gggactgcgc ctgcaggaac ccctggtgcc ccacccctgg
     3241 ctggcatggc cattgtcaag gaggaggaga cggaggctgc cattggagcc cctcctactg
     3301 ccactgaggg ccctgagacc aaacctgtgc ttatggctct tgcggagggt cctggtgctg
     3361 agggtccccg cctggcctca cctagtggca gcaccagctc agggctggag gtggtggctc
     3421 ctgagggtac ctcagcccca ggtggtggcc cgggaaccct ggatgacagt gccaccattt
     3481 gccgtgtctg ccagaagcca ggcgatctgg ttatgtgcaa ccagtgtgag ttttgtttcc
     3541 acctggactg tcacctgccg gccctgcagg atgtaccagg ggaggagtgg agctgctcac
     3601 tctgccatgt gctccctgac ctgaaggagg aggatggcag cctcagcctg gatggtgcag
     3661 acagcactgg cgtggtggcc aagctctcac cagccaacca gcggaaatgt gagcgtgtac
     3721 tgctggccct attctgtcac gaaccctgcc gccccctgca tcagctggct accgactcca
     3781 ccttctccct ggaccagccc ggtggcaccc tggatctgac cctgatccgt gcccgcctcc
     3841 aggagaagtt gtcacctccc tacagctccc cacaggagtt tgcccaggat gtgggccgca
     3901 tgttcaagca attcaacaag ttaactgagg acaaggcaga cgtgcagtcc atcatcggcc
     3961 tgcagcgctt cttcgagacg cgcatgaacg aggccttcgg tgacaccaag ttctctgctg
     4021 tgctggtgga gcccccgccg atgagcctgc ctggtgctgg cctgagttcc caggagctgt
     4081 ctggtggccc tggtgatggc cccgtttaaa cgaattcggg ctcggtaccc ggggatcctc
     4141 tagagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg aaaggaagct
     4201 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ggccgcttcg
     4261 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     4321 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4381 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     4441 gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca
     4501 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4561 ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga
     4621 acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4681 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4741 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4861 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4921 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4981 ctaggctttt gcaaaaagct taattaactg ttgacaatta atcatcggca tagtatatcg
     5041 gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat gattgaacaa
     5101 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5161 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5221 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5281 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5341 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5401 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5461 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5521 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5581 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     5641 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5701 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     5761 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5821 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5881 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat
     5941 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     6001 atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     6061 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacccggt atcagctcac
     6121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6661 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6781 gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt
     6841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6901 ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaaatacagg aacgcacgct
     6961 ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg
     7021 ggggtaatgt ggcctgtacc ctctggttgc ataggtattc atacggttaa aatttatcag
     7081 gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga
     7141 tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga ttatggtaaa tccacttact
     7201 gtctgccctc gtagccatcg agataaaccg cagtactccg gccacgatgc gtccggcgta
     7261 gaggatcgag atct
//
