LOCUS       Exported                8822 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Previously pFdR4Hc
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3360..4661
                     /label=TEAD4_NM_003213
     misc_feature    1..193
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    complement(194..241)
                     /label=MCS 2
     misc_feature    complement(279..286)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(287..1798)
                     /label=YAP1
                     /note="YAP1"
     misc_feature    complement(1800..1807)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(1808..1819)
                     /label=linker
                     /note="linker"
     misc_feature    complement(1820..2332)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    2346..2372
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2373..2389)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2392..2437)
                     /label=Minimal CMV Promoter
     misc_feature    2415..2437
                     /label=core
                     /note="core"
     promoter        2448..3037
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          3152..3284
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        3328..3347
                     /label=T7 promoter
     misc_feature    3351..3358
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3360..4661
                     /label=TEAD4
                     /note="TEAD4"
     misc_feature    4668..4712
                     /label=HT7 Linker C terminal
     misc_feature    4713..5603
                     /label=HaloTag
     misc_feature    5630..7972
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     polyA_signal    5681..5902
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(5926..5959)
                     /label=Cre site
                     /note="Cre site"
     promoter        5968..6034
                     /label=EM7 bacterial promoter
     CDS             6048..6842
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    6849..6886
                     /label=Untitled
     misc_feature    6945..7867
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    7122
                     /label=C2129
                     /note="C2129"
     polyA_signal    complement(7889..7936)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(7938..7972)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    7948..8801
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    7948..7982
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(7960..8358)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(8383..8801)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(8776..8801)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    8793..8801
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    8802..8822
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    8802
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gtgtttctag aggatccccg ggtaccgagc ccgaattcgt ttaaactaac catgtaagaa
      301 agctttcttt atctagcttg gtggcagcca aaacagactc catgtcatta aggatgtcag
      361 aactcaaagc ttcctgcaga cttggcatca gctcctctcc ttctatgttc attccatctc
      421 cttccagtgt tccaaggtcc acatttgtcc caggaatggc ttcaaggtag tctgggaaac
      481 ggttctgctg tgagggcagg gtgctttggt tgatagtatc acctgtatcc atctcatcca
      541 cactgttcag gaagtcatct ggggttcgag ggacactgta gctgctcatg cttagtccac
      601 tgtctgtact ctcatctcga gagtgatagg tgccactgtt aaggaaagga tctgagctat
      661 tggtcgtcat tgttctcaat tcctgagaca tcccgggaga agacactgga ttttgagtcc
      721 caccatcctg ctccagtgtt ggtaactggc tacgcagggc taactcctga cattttggag
      781 aatttgctgt gctgggattg atattccgca ttgcctgccg aagcagttct tgctgtttca
      841 gccgcagcct ctccttctcc atctgcagtt gctgcagtcg catctgttgc tgctggttgg
      901 agttgctgcc acccatgacg cctccctgtg ggctctgggg agccaggggt ggtggctgtt
      961 tcactggagc actctgactg attctctggt tcatggcaaa acgagggtca agccttgggt
     1021 ctagccaaga ggtggtcttg ttcttatggt ttatatagta aatttctcca tcctgagtca
     1081 tggcttgttc ccatccatca ggaagaggac ctgaagccga gttcatcata ttctgctgca
     1141 ctggtggact ggtgggggct gtgacgttca tctgggacag catggccttc ctggggtcct
     1201 gccatgttgt tgtctgatcg atgtgattta agaagtatct ctgaccagaa gatgtctttg
     1261 ccatctccca acctgctggc agaggtacat catcaggtat ctcaaaagaa gactgtcgaa
     1321 gatgctgagc tgtgggtgta gctgctgggc cagagactac tccagtgggg gtcagtgtcc
     1381 caggagaaac agctcccaac tgcagagaag ctggagagga atgagctcga acatgctgtg
     1441 gagtcagggc tcctgcagtg cctgcatcag tactggcctg tcgggagtgg gatttgggct
     1501 ccggcggctt gaagaaggag tcgggcagct tccggagcct catgggcacg gtctggggca
     1561 cgttggccgt cttggggttc atgacggcgt tgaagagcgc ctccaggtcg gtctccgagt
     1621 ccccgcggac gtgcacgatc tgatgcccgg cggggggtgc ctgcggcgcc gcctgggtcg
     1681 ccgcgggtgc cggttgcccg ggtccggacg gcgggccctg cccctgcggg ggctgcgaag
     1741 gcggctgccc ttggccctgg ggggccggtt gaggcggcgg ctgctgcccg ggatccatgg
     1801 cgatcgcgcc gctcgagccc gccagaatgc gttcgcacag ccgccagccg gtcactccgt
     1861 tgatggttac tcggaacagc agggagccgt cggggttgat caggcgctcg tcgataattt
     1921 tgttgccgtt ccacagggtc cctgttacag tgatcttttt gccgtcgaac acggcgatgc
     1981 cttcatacgg ccgtccgaaa tagtcgatca tgttcggcgt aaccccgtcg attaccagtg
     2041 tgccatagtg caggatcacc ttaaagtgat gatcatccac agggtacacc accttaaaaa
     2101 ttttttcgat ctggcccatt tggtcgccgc tcagaccttc atacgggatg atgacatgga
     2161 tgtcgatctt cagcccattt tcaccgctca ggacaatcct ttggatcgga gttacggaca
     2221 ccccgagatt ctgaaacaaa ctggacacac ctccctgttc aaggacttgg tccaggttgt
     2281 agccggctgt ctgtcgccag tccccaacga aatcttcgag tgtgaagacc atggtgagcg
     2341 ctagctgcaa aaagaacaag tagcttgtat tctatagtgt cacctaaatg ggcgatctga
     2401 cggttcacta aaccagctct gcttatatag gcctccctgg cgcgccacta gttattaata
     2461 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2521 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2581 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2641 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
     2701 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
     2761 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2821 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2881 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2941 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3001 ctatataagc agagctggtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
     3061 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
     3121 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
     3181 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
     3241 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
     3301 caattacagc tcttaaggct agagtattaa tacgactcac tatagggcta gcgatcgcca
     3361 tggagggcac ggccggcacc attacctcca acgagtggag ctctcccacc tcccctgagg
     3421 ggagcaccgc ctctgggggc agtcaggcac tggacaagcc catcgacaat gacgcagagg
     3481 gcgtgtggag cccggatatt gagcagagtt tccaggaggc cctcgccatc tacccgccct
     3541 gtggcaggcg caaaatcatc ctgtcggacg agggcaagat gtatggtcgg aacgagctga
     3601 ttgcccgcta catcaagctc cggacaggga agacccgcac caggaagcag gtctccagcc
     3661 acatccaggt gctggctcgt cgcaaagctc gcgagatcca ggccaagcta aaggaccagg
     3721 cagctaagga caaggccctg cagagcatgg ctgccatgtc gtctgcacag atcatctccg
     3781 ccacggcctt ccacagtagc atggccctcg cccggggccc cggccgccca gcagtctcag
     3841 ggttttggca aggagctttg ccaggccaag ccggaacgtc ccatgatgtg aagcctttct
     3901 ctcagcaaac ctatgctgtc cagcctccgc tgcctctgcc agggtttgag tctcctgcag
     3961 ggcccgcccc atcgccctct gcgcccccgg cacccccatg gcagggccgc agcgtggcca
     4021 gctccaagct ctggatgttg gagttctctg ccttcctgga gcagcagcag gacccggaca
     4081 cgtacaacaa gcacctgttc gtgcacattg gccagtccag cccaagctac agcgacccct
     4141 acctcgaagc cgtggacatc cgccaaatct atgacaaatt cccggagaaa aagggtggac
     4201 tcaaggatct cttcgaacgg ggaccctcca atgccttttt tcttgtgaag ttctgggcag
     4261 acctcaacac caacatcgag gatgaaggca gctccttcta tggggtctcc agccagtatg
     4321 agagccccga gaacatgatc atcacctgct ccacgaaggt ctgctctttc ggcaagcagg
     4381 tggtggagaa agttgagaca gagtatgctc gctatgagaa tggacactac tcttaccgca
     4441 tccaccggtc cccgctctgt gagtacatga tcaacttcat ccacaagctc aagcacctcc
     4501 ctgagaagta catgatgaac agcgtgctgg agaacttcac catcctgcag gtggtcacca
     4561 acagagacac acaggagacc ttgctgtgca ttgcctatgt ctttgaggtg tcagccagtg
     4621 agcacggggc tcagcaccac atctacaggc tggtgaaaga agtttctctc gagccaacca
     4681 ctgaggatct gtactttcag agcgataacg atggatccga aatcggtact ggctttccat
     4741 tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc
     4801 gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca
     4861 acatcatccc gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg
     4921 gcaaatccga caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg
     4981 ccttcatcga agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg
     5041 ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg
     5101 agttcatccg ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc
     5161 aggccttccg caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg
     5221 agggtacgct gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc
     5281 gcgagccgtt cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc
     5341 caatcgccgg tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc
     5401 accagtcccc tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg
     5461 ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg
     5521 gtctgaatct gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc
     5581 tgtctactct ggagatttcc ggttaataga attctagagt cgacctgcag gcatgcaagc
     5641 tgatttggct gctgccaccg ctgagcaagg ccgcttcgag cagacatgat aagatacatt
     5701 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     5761 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     5821 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag
     5881 taaaacctct acaaatgtgg taaaatctta attaagaatt ctaatataac ttcgtatagc
     5941 atacattata cgaagttatg gatccactgt tgacaattaa tcatcggcat agtatatcgg
     6001 catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg attgaacaag
     6061 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6121 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6181 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6241 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6301 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6361 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6421 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6481 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6541 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6601 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6661 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6721 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6781 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6841 gattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat
     6901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtcaaaca tgagaattac
     6961 aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa
     7021 atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc
     7081 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc
     7141 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt
     7201 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt
     7261 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata
     7321 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     7381 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     7441 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     7501 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     7561 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     7621 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     7681 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     7741 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     7801 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac
     7861 tccgctagcg ctgatgtccg gaatcgtcac acaaaaaacc aacacacaga tgtaatgaaa
     7921 ataaagatat tttattgcgg ccatcgtgat ggctagagtt tagccctccc acacataacc
     7981 agagggcagc aattcacgaa tcccaactgc cgtcggctgt ccatcactgt ccttcactat
     8041 ggctttgatc ccaggatgca gatcgagaag cacctgtcgg caccgtccgc aggggctcaa
     8101 gatgcccctg ttctcatttc cgatcgcgac gatacaagtc aggttgccag ctgccgcagc
     8161 agcagcagtg cccagcacca cgagttctgc acaaggtccc ccagtaaaat gatatacatt
     8221 gacaccagtg aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc
     8281 agagatgggg atgctgttga ttgtagccgt tgctctttca atgagggtgg attcttcttg
     8341 agacaaaggc ttggccatgg tggcgctagt gtcagaagaa tcgagctttt tgcaaaagcc
     8401 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     8461 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     8521 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     8581 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     8641 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     8701 acaccctaac tgacacacat tccacagctg gttctttccg cctcagaagg tacctaacca
     8761 agttcctctt tcagaggtta tttcaggcca tggtgctgcg caagacgcgt taattaagac
     8821 gt
//
