LOCUS       Exported                6630 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Previously pFdR4Hc
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1168..2469
                     /label=TEAD4_NM_003213
     misc_feature    1..246
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    complement(192..254)
                     /label=MCS 2
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1159..1166
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1168..2469
                     /label=TEAD4
                     /note="TEAD4"
     misc_feature    2476..2520
                     /label=HT7 Linker C terminal
     misc_feature    2521..3411
                     /label=HaloTag
     misc_feature    3438..5780
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     polyA_signal    3489..3710
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(3734..3767)
                     /label=Cre site
                     /note="Cre site"
     promoter        3776..3842
                     /label=EM7 bacterial promoter
     CDS             3856..4650
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    4657..4694
                     /label=Untitled
     misc_feature    4753..5675
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    4930
                     /label=C2129
                     /note="C2129"
     polyA_signal    complement(5697..5744)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(5746..5780)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    5756..6609
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    5756..5790
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(5768..6166)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(6191..6609)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(6584..6609)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    6601..6609
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    6610..6630
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    6610
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc gatcgccatg gagggcacgg ccggcaccat tacctccaac
     1201 gagtggagct ctcccacctc ccctgagggg agcaccgcct ctgggggcag tcaggcactg
     1261 gacaagccca tcgacaatga cgcagagggc gtgtggagcc cggatattga gcagagtttc
     1321 caggaggccc tcgccatcta cccgccctgt ggcaggcgca aaatcatcct gtcggacgag
     1381 ggcaagatgt atggtcggaa cgagctgatt gcccgctaca tcaagctccg gacagggaag
     1441 acccgcacca ggaagcaggt ctccagccac atccaggtgc tggctcgtcg caaagctcgc
     1501 gagatccagg ccaagctaaa ggaccaggca gctaaggaca aggccctgca gagcatggct
     1561 gccatgtcgt ctgcacagat catctccgcc acggccttcc acagtagcat ggccctcgcc
     1621 cggggccccg gccgcccagc agtctcaggg ttttggcaag gagctttgcc aggccaagcc
     1681 ggaacgtccc atgatgtgaa gcctttctct cagcaaacct atgctgtcca gcctccgctg
     1741 cctctgccag ggtttgagtc tcctgcaggg cccgccccat cgccctctgc gcccccggca
     1801 cccccatggc agggccgcag cgtggccagc tccaagctct ggatgttgga gttctctgcc
     1861 ttcctggagc agcagcagga cccggacacg tacaacaagc acctgttcgt gcacattggc
     1921 cagtccagcc caagctacag cgacccctac ctcgaagccg tggacatccg ccaaatctat
     1981 gacaaattcc cggagaaaaa gggtggactc aaggatctct tcgaacgggg accctccaat
     2041 gccttttttc ttgtgaagtt ctgggcagac ctcaacacca acatcgagga tgaaggcagc
     2101 tccttctatg gggtctccag ccagtatgag agccccgaga acatgatcat cacctgctcc
     2161 acgaaggtct gctctttcgg caagcaggtg gtggagaaag ttgagacaga gtatgctcgc
     2221 tatgagaatg gacactactc ttaccgcatc caccggtccc cgctctgtga gtacatgatc
     2281 aacttcatcc acaagctcaa gcacctccct gagaagtaca tgatgaacag cgtgctggag
     2341 aacttcacca tcctgcaggt ggtcaccaac agagacacac aggagacctt gctgtgcatt
     2401 gcctatgtct ttgaggtgtc agccagtgag cacggggctc agcaccacat ctacaggctg
     2461 gtgaaagaag tttctctcga gccaaccact gaggatctgt actttcagag cgataacgat
     2521 ggatccgaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
     2581 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
     2641 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
     2701 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
     2761 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
     2821 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
     2881 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
     2941 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
     3001 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
     3061 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag
     3121 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
     3181 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
     3241 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
     3301 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
     3361 ctgatcggca gcgagatcgc gcgctggctg tctactctgg agatttccgg ttaatagaat
     3421 tctagagtcg acctgcaggc atgcaagctg atttggctgc tgccaccgct gagcaaggcc
     3481 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3541 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3601 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3661 ggagatgtgg gaggtttttt taagcaagta aaacctctac aaatgtggta aaatcttaat
     3721 taagaattct aatataactt cgtatagcat acattatacg aagttatgga tccactgttg
     3781 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     3841 cccaggaggc agatcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3901 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3961 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4021 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4081 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4141 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4201 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4261 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4321 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4381 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4441 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4501 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4561 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4621 ttctatcgcc ttcttgacga gttcttctga ttcgaaatga ccgaccaagc gacgcccaac
     4681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4741 gaaagaacat gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc
     4801 tacatttgaa gagataaatt gcactgaaat ctagaaatat tttatctgat taataagatg
     4861 atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc
     4921 cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg
     4981 gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga
     5041 cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat
     5101 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa
     5161 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     5221 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     5281 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
     5341 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     5401 agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg
     5461 catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga
     5521 ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct
     5581 gacgcaccgg tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc
     5641 agtgccaaca tagtaagcca gtatacactc cgctagcgct gatgtccgga atcgtcacac
     5701 aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc atcgtgatgg
     5761 ctagagttta gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg
     5821 tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca
     5881 cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga
     5941 tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac
     6001 aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga
     6061 gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg
     6121 ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg gcgctagtgt
     6181 cagaagaatc gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     6241 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     6301 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     6361 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6421 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     6481 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
     6541 tctttccgcc tcagaaggta cctaaccaag ttcctctttc agaggttatt tcaggccatg
     6601 gtgctgcgca agacgcgtta attaagacgt
//
