LOCUS       Exported                7569 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC27K(pFC27K)      5527 bp    DNA   circular           
            3/27/2014

            DEFINITION  Current Name: pFC27K (pFC27K) Old/Alternate 
            Name(s):pFC27K (backbone)
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..3482
                     /label=VCP
                     /note="VCP"
     misc_feature    3489..3533
                     /label=HT7 Linker C terminal
     misc_feature    3534..4424
                     /label=HT7 tag C terminal
     polyA_signal    4558..4779
                     /label=Sv40 late polyadenylation signal
     promoter        4878..5296
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        5304..5370
                     /label=EM7 bacterial promoter
     CDS             5384..6178
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    6242..6290
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      6526..6562
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggct tctggagccg
     1081 attcaaaagg tgatgaccta tcaacagcca ttctcaaaca gaagaaccgt cccaatcggt
     1141 taattgttga tgaagccatc aatgaggaca acagtgtggt gtccttgtcc cagcccaaga
     1201 tggatgaatt gcagttgttc cgaggtgaca cagtgttgct gaaaggaaag aagagacgag
     1261 aagctgtttg catcgtcctt tctgatgata cttgttctga tgagaagatt cggatgaata
     1321 gagttgttcg gaataacctt cgtgtacgcc taggggatgt catcagcatc cagccatgcc
     1381 ctgatgtgaa gtacggcaaa cgtatccatg tgctgcccat tgatgacaca gtggaaggca
     1441 ttactggtaa tctcttcgag gtatacctta agccgtactt cctggaagcg tatcgaccca
     1501 tccggaaagg agacattttt cttgtccgtg gtgggatgcg tgctgtggag ttcaaagtgg
     1561 tggaaacaga tcctagccct tattgcattg ttgctccaga cacagtgatc cactgcgaag
     1621 gggagcctat caaacgagag gatgaggaag agtccttgaa tgaagtaggg tatgatgaca
     1681 ttggtggctg caggaagcag ctagctcaga taaaggagat ggtggaactg cccctgagac
     1741 atcctgccct ctttaaggca attggtgtga agcctcctag aggaatcctg ctttacggac
     1801 ctcctggaac aggaaagacc ctgattgctc gagctgtagc aaatgagact ggagccttct
     1861 tcttcttgat caatggtcct gagatcatga gcaaattggc tggtgagtct gagagcaacc
     1921 ttcgtaaagc ctttgaggag gctgagaaga atgctcctgc catcatcttc attgatgagc
     1981 tagatgccat cgctcccaaa agagagaaaa ctcatggcga ggtggagcgg cgcattgtat
     2041 cacagttgtt gaccctcatg gatggcctaa agcagagggc acatgtgatt gttatggcag
     2101 caaccaacag acccaacagc attgacccag ctctacggcg atttggtcgc tttgacaggg
     2161 aggtagatat tggaattcct gatgctacag gacgcttaga gattcttcag atccatacca
     2221 agaacatgaa gctggcagat gatgtggacc tggaacaggt agccaatgag actcacgggc
     2281 atgtgggtgc tgacttagca gccctgtgct cagaggctgc tctgcaagcc atccgcaaga
     2341 agatggatct cattgaccta gaggatgaga ccattgatgc cgaggtcatg aactctctag
     2401 cagttactat ggatgacttc cggtgggcct tgagccagag taacccatca gcactgcggg
     2461 aaaccgtggt agaggtgcca caggtaacct gggaagacat cgggggccta gaggatgtca
     2521 aacgtgagct acaggagctg gtccagtatc ctgtggagca cccagacaaa ttcctgaagt
     2581 ttggcatgac accttccaag ggagttctgt tctatggacc tcctggctgt gggaaaactt
     2641 tgttggccaa agccattgct aatgaatgcc aggccaactt catctccatc aagggtcctg
     2701 agctgctcac catgtggttt ggggagtctg aggccaatgt cagagaaatc tttgacaagg
     2761 cccgccaagc tgccccctgt gtgctattct ttgatgagct ggattcgatt gccaaggctc
     2821 gtggaggtaa cattggagat ggtggtgggg ctgctgaccg agtcatcaac cagatcctga
     2881 cagaaatgga tggcatgtcc acaaaaaaaa atgtgttcat cattggcgct accaaccggc
     2941 ctgacatcat tgatcctgcc atcctcagac ctggccgtct tgatcagctc atctacatcc
     3001 cacttcctga tgagaagtcc cgtgttgcca tcctcaaggc taacctgcgc aagtccccag
     3061 ttgccaagga tgtggacttg gagttcctgg ctaaaatgac taatggcttc tctggagctg
     3121 acctgacaga gatttgccag cgtgcttgca agctggccat ccgtgaatcc atcgagagtg
     3181 agattaggcg agaacgagag aggcagacaa acccatcagc catggaggta gaagaggatg
     3241 atccagtgcc tgagatccgt cgagatcact ttgaagaagc catgcgcttt gcgcgccgtt
     3301 ctgtcagtga caatgacatt cggaagtatg agatgtttgc ccagaccctt cagcagagtc
     3361 ggggctttgg cagcttcaga ttcccttcag ggaaccaggg tggagctggc cccagtcagg
     3421 gcagtggagg cggcacaggt ggcagtgtat acacagaaga caatgatgat gacctgtatg
     3481 gcgtttctct cgagccaacc actgaggatc tgtactttca gagcgataac gatggatccg
     3541 aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc gagcgcatgc
     3601 actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac ggtaacccga
     3661 cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat cgctgcattg
     3721 ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat ttcttcgacg
     3781 accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag gtcgtcctgg
     3841 tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat ccagagcgcg
     3901 tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac gaatggccag
     3961 aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc aagctgatca
     4021 tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg
     4081 aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt
     4141 ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg
     4201 aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc tggggcaccc
     4261 caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg cctaactgca
     4321 aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg gacctgatcg
     4381 gcagcgagat cgcgcgctgg ctgtctactc tggagatttc cggttaatag aattctagag
     4441 tcgacctgca ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt
     4501 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcggccg cttcgagcag
     4561 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     4621 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     4681 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     4741 aggttttttt aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata
     4801 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4861 accgcatacg cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     4921 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     4981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5041 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5101 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5161 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5221 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5281 cttttgcaaa aagcttaatt aactgttgac aattaatcat cggcatagta tatcggcata
     5341 gtataatacg acaaggtgag gaactaaacc caggaggcag atcatgattg aacaagatgg
     5401 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5461 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5521 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5581 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5641 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5701 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5761 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5821 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5881 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     5941 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6001 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6061 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6121 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6181 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg
     6241 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc
     6301 gataaggatc ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt
     6361 catccggggt cagcaccgtt tctgcggact ggctttctac ccggtatcag ctcactcaaa
     6421 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6721 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6961 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7081 caagcagcag attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac
     7141 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7201 aaaaaggatc ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg
     7261 gcccttcgct gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt
     7321 aatgtggcct gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg
     7381 attgcggcag tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg
     7441 ctgaacgcgt tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg
     7501 ccctcgtagc catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga
     7561 tcgagatct
//
