LOCUS       Exported                5818 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(1..188)
                     /label=SV40\polyA\signal
     misc_feature    complement(192..254)
                     /label=MCS 2
     misc_feature    192..199
                     /label=NotI
                     /note="NotI"
     misc_feature    197..218
                     /label=HnHc_dR4_1
                     /note="HnHc_dR4_1"
     promoter        255..845
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     misc_feature    620..638
                     /label=HnHc_dR4_2
                     /note="HnHc_dR4_2"
     intron          960..1092
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1136..1155
                     /label=T7 promoter
     misc_feature    1170..2060
                     /label=HaloTag
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    complement(1210..1230)
                     /label=HTnR
                     /note="HTnR"
     misc_feature    1607..1625
                     /label=HnHc_dR4_3
                     /note="HnHc_dR4_3"
     misc_feature    2061..2099
                     /label=HT7 Linker N terminal
     misc_feature    2073..2093
                     /label=TEV protease recognition sequence
     misc_feature    2100..2104
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2105..2107
                     /label=SgfI
                     /note="SgfI"
     misc_feature    2109..2747
                     /label=VHL
                     /note="VHL"
     misc_feature    2748..2751
                     /label=PmeI
                     /note="PmeI"
     misc_feature    2752..2755
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2787..2802
                     /label=HnHc_dR4_4
                     /note="HnHc_dR4_4"
     misc_feature    2830..2831
                     /label=BlpI
                     /note="BlpI"
     polyA_signal    2851..3072
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(3096..3129)
                     /label=Cre site
                     /note="Cre site"
     promoter        3138..3204
                     /label=EM7 bacterial promoter
     CDS             3218..4012
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    3592..3609
                     /label=HnHc_dR4_5
                     /note="HnHc_dR4_5"
     rep_origin      4167..4203
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    4189..4211
                     /label=HnHc_dR4_6
                     /note="HnHc_dR4_6"
     misc_feature    4785..4803
                     /label=HnHc_dR4_7
                     /note="HnHc_dR4_7"
     polyA_signal    complement(4885..4932)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(4956..5354)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(5379..5797)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    5388..5405
                     /label=HnHc_dR4_8
                     /note="HnHc_dR4_8"
     misc_feature    join(5789..5818,1..3)
                     /label=RSGBFB15 (primer)
                     /note="RSGBFB15 (primer)"
ORIGIN
        1 cggcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc cgcggccgca atacgtcgac gttatcagct gacttcgtac gagagcctag
      241 gattatggcg cgccactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      841 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      901 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      961 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     1021 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     1081 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtattaata
     1141 cgactcacta tagggctagc aaagccacca tggcagaaat cggtactggc tttccattcg
     1201 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     1261 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     1321 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     1381 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     1441 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     1501 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     1561 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     1621 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     1681 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     1741 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     1801 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     1861 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     1921 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     1981 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     2041 cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag agcgataacg
     2101 cgatcgccat gccccggagg gcggagaact gggacgaggc cgaggtaggc gcggaggagg
     2161 caggcgtcga agagtacggc cctgaagaag acggcgggga ggagtcgggc gccgaggagt
     2221 ccggcccgga agagtccggc ccggaggaac tgggcgccga ggaggagatg gaggccgggc
     2281 ggccgcggcc cgtgctgcgc tcggtgaact cgcgcgagcc ctcccaggtc atcttctgca
     2341 atcgcagtcc gcgcgtcgtg ctgcccgtat ggctcaactt cgacggcgag ccgcagccct
     2401 acccaacgct gccgcctggc acgggccgcc gcatccacag ctaccgaggt cacctttggc
     2461 tcttcagaga tgcagggaca cacgatgggc ttctggttaa ccaaactgaa ttatttgtgc
     2521 catctctcaa tgttgacgga cagcctattt ttgccaatat cacactgcca gtgtatactc
     2581 tgaaagagcg atgcctccag gttgtccgga gcctagtcaa gcctgagaat tacaggagac
     2641 tggacatcgt caggtcgctc tacgaagatc tggaagacca cccaaatgtg cagaaagacc
     2701 tggagcggct gacacaggag cgcattgcac atcaacggat gggagatgtt taaacgaatt
     2761 cgggctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc tgatttggct
     2821 gctgccaccg ctgagcaagg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     2881 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2941 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3001 attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag taaaacctct
     3061 acaaatgtgg taaaatctta attaagaatt ctaatataac ttcgtatagc atacattata
     3121 cgaagttatg gatccactgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     3181 tacgacaagg tgaggaacta aacccaggag gcagatcatg attgaacaag atggattgca
     3241 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     3301 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     3361 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     3421 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3481 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3541 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     3601 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     3661 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3721 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     3781 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3841 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3901 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3961 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gattcgaaat
     4021 gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat acggttatcc
     4081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     4621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4741 agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4861 atccttttat agtccggaat cgtcacacaa aaaaccaaca cacagatgta atgaaaataa
     4921 agatatttta ttgcggccat cgtgatggct agagtttagc cctcccacac ataaccagag
     4981 ggcagcaatt cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatggct
     5041 ttgatcccag gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg
     5101 cccctgttct catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca
     5161 gcagtgccca gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca
     5221 ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag
     5281 atggggatgc tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac
     5341 aaaggcttgg ccatggtggc gctagtgtca gaagaatcga gctttttgca aaagcctagg
     5401 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     5461 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     5521 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     5581 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     5641 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     5701 cctaactgac acacattcca cagctggttc tttccgcctc agaaggtacc taaccaagtt
     5761 cctctttcag aggttatttc aggccatggt gctgcgcaag acgcgttaat taagacgt
//
