LOCUS       Exported                7478 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    805..2743
                     /label=NSD3 WT
                     /note="NSD3 WT"
     misc_feature    complement(2745..2752)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2757..2783
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2784..2800)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2803..2848)
                     /label=Minimal CMV Promoter 2
     misc_feature    2826..2848
                     /label=core
                     /note="core"
     promoter        2858..3412
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        3430..3496
                     /label=EM7 bacterial promoter
     CDS             3510..4169
                     /label=Chloramphenicol resistance
     rep_origin      4338..4374
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5056..5103)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(5127..5921)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5935..6229)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(6300..7478)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacgt ccacagtttc ctcaatcgct gcggagacgg
      841 agctgtcact gaatctgaca gagccctgca ctccccggtc cgccgaccct ttctgtaatc
      901 cagtcttcac tgtagcctga ggaactgttt caacctgctc ctttttgatc ttcttctttg
      961 gcacaacctc agtggatttc tctgattcag aacgagttct aattgatctt ctctgttgct
     1021 tcttttctac tgagcctgta gaaccagatg ttgcttcagg agatgataca ctctgcgttg
     1081 gcttttcatt tctctggttt ggtgtagaaa ttataagcct gtcttgcccc ctgacactta
     1141 tttctgtttt gttaccaatt ccctttgttg aataaacaaa ttgatcgata aatttcccat
     1201 cccctgtagc attctgaaga gcaaacactt gttcaatttt cacaactgga gacatgttac
     1261 acttctgcaa atccaggctc cctttgtgca tcgtaatgga agctggtaag gatttccttg
     1321 ctgccgcagt tttccaggct attttaacag gcggtggctc ttcctcttcc gcacttgtgt
     1381 gccgcctctg gctatgtctc cgaatttcag tacttgagag tgaggaggcc acctcccctg
     1441 cattggtctg ttctggctga gtattcagca cagatcttgg tcgtcgggtt tttttaactt
     1501 cggttttgga ggcaacactc ttttttgctt gggataaagc ctcttcaggc tgtttatcaa
     1561 tgtaaataaa agtatactgt tctattcttt cttctcgagt cattttcaat gctttctctg
     1621 catgggcaat gccaatatcc cactgagcac gttctctctg aggtcggggt ttccgaatct
     1681 tttgtttctc agagtgattg ctggcttgtt tggttgcctc agccagtaat tcttcatact
     1741 gtttatgacc tttatactct cgtacccgtt tttcatgaac ccacgccctc tctggctggt
     1801 tgctaaaaaa ctggacatga tattctcggg cacctcttgt gttaatttta gtatgaacct
     1861 caagctgggg atcacttgaa accatacaag gccaccaagg ataggttccc accttggacc
     1921 acacaagatc gccaacctga aacttaacac cagtggacac ttccgttgtt ggaacagaag
     1981 atagtattgg ctgaactggg gcttcctctt ttagtactgg ttcttccctt ggtttttctg
     2041 atacagtgtc aaccctctca tttggtctat tttgttcctc tggttctaat ttggggattt
     2101 tgtgtgactt gcgctcttca gatcttgatg agtcatgctt gttgcttttt ttcctctttt
     2161 cttttctgct ttcatgcttt gatttcgtgt gctcacttgc ctgtacttca tttaaaaggt
     2221 ctccacaaag ggaagactca aacaattccc tgccattctg gatagttttg gttattttta
     2281 gtttaatttc aggtgagcca gtcttctttg gaatcacagt ttgtggtacc gaaggaggag
     2341 gtggtggctg tggaggggaa ggtttttcca gaatttcatg tggtcttgtg tttggaattt
     2401 ctgaatgata atagtcagtg gggctaaagt ttctaactgc accaaagcca ttggctgacc
     2461 cattaggata ctgattatat gactggtatt tggtttgagt ttcatacaca ctgattgatg
     2521 atggataccc atttgtgagt ggaggaagat cttctgttgt agctgggtac tgaaagcctt
     2581 gctgcaaagt agcttcatat ggtgtctggc caccatcttc agcaatgtca ctgttgttat
     2641 caaaggcatc ctcctgacgg atgttggcgg agtcaatgag ttgaggtggt tgctgaattg
     2701 tgtttcccat gatcccttgc atgaaagaga aagagaaatc catggcgatc gctagctgca
     2761 aaaagaacaa gtagcttgta ttctatagtg tcacctaaat gggcgatctg acggttcact
     2821 aaaccagctc tgcttatata ggcctccctg gcgcgccact agttattaat agtaatcaat
     2881 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2941 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     3001 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     3061 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
     3121 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
     3181 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     3241 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     3301 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     3361 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tcaaatcgaa
     3421 ttctaatact gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     3481 ggtgaggaac taaacccagg aggcagatca tggagaaaaa aatcactgga tataccaccg
     3541 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     3601 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     3661 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     3721 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     3781 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     3841 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     3901 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     3961 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     4021 tcactatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     4081 ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     4141 actgcgatga gtggcagggc ggggcgtaag cgggactctg gggttcgaaa tgaccgacca
     4201 agcgacgccc aaccggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4261 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4321 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4381 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4441 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4501 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4561 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4621 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4681 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4741 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     4801 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4861 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4921 ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4981 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5041 tagtccggaa tcgtcacaca aaaaaccaac acacagatgt aatgaaaata aagatatttt
     5101 attgcggcca tcgtgatggc tagagttcag aagaactcgt caagaaggcg atagaaggcg
     5161 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     5221 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     5281 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     5341 ggcaagcagg catcgccgtg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     5401 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     5461 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     5521 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     5581 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     5641 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     5701 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     5761 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5821 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5881 gcggccggag aacctgcgtg caatccatct tgttcaatca tggtggcatc gattcccggt
     5941 caacatccag tacacctccg gcaccaccgg cttccccaag ggcgccacgc tctcccacca
     6001 caacatcctc aacaacggct actgggtggg ccgcacggtc ggctacacgg agcaggaccg
     6061 ggtgtgtctg ccggtgccct tctaccactg cttcggcatg gtcatgggca acttgggcgc
     6121 cacctcccac gccgcctgca tcgtcatccc ggccccgtcc ttcgagccgg cggcgacgct
     6181 ggaggcggtg cagcgggaga ggtgcacgtc cctgtacggc gtccccacga agccgaattc
     6241 caccacactg gactagatgc atgctcgaca gtgtgatgga tatctgcaga attcggcttt
     6301 cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac
     6361 caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc
     6421 ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
     6481 caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc
     6541 cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt
     6601 gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctgcctgca
     6661 gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt
     6721 gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcca
     6781 gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa
     6841 catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca
     6901 gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca
     6961 ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg
     7021 tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga
     7081 ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc
     7141 tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca
     7201 taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg
     7261 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     7321 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     7381 caccttctct aggcaccggt tcaattgccg acccctcccc ccaacttctc ggggactgtg
     7441 ggcgatgtgc gctctgccca ctgacgggca ccggagcc
//
