LOCUS       Exported                6465 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..2786
                     /label=ACT1
                     /note="ACT1"
     misc_feature    2793..2807
                     /label=linker
                     /note="linker"
     misc_feature    2808..3317
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    3454..3675
                     /label=Sv40 late polyadenylation signal
     promoter        3774..4192
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4200..4266
                     /label=EM7 bacterial promoter
     CDS             4280..5074
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5138..5186
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5422..5458
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcct cctcagcttc
     1081 aagaaactag aatgaaccga agcattcctg tggaggttga tgaatcagaa ccatacccaa
     1141 gtcagttgct gaaaccaatc ccagaatatt ccccggaaga ggaatcagaa ccacctgctc
     1201 caaatataag gaacatggca cccaacagct tgtctgcacc cacaatgctt cacaattcct
     1261 ccggagactt ttctcaagct cactcaaccc tgaaacttgc aaatcaccag cggcctgtat
     1321 cccggcaggt cacctgcctg cgcactcaag ttctggagga cagtgaagac agtttctgca
     1381 ggagacaccc aggcctgggc aaagctttcc cttctgggtg ctctgcagtc agcgagcctg
     1441 cgtctgagtc tgtggttgga gccctccctg cagagcatca gttttcattt atggaaaaac
     1501 gtaatcaatg gctggtatct cagctttcag cggcttctcc tgacactggc catgactcag
     1561 acaaatcaga ccaaagttta cctaatgcct cagcagactc cttgggcggt agccaggaga
     1621 tggtgcaacg gccccagcct cacaggaacc gagcaggcct ggatctgcca accatagaca
     1681 cgggatatga ttcccagccc caggatgtcc tgggcatcag gcagctggaa aggcccctgc
     1741 ccctcacctc cgtgtgttac ccccaggacc tccccagacc tctcaggtcc agggagttcc
     1801 ctcagtttga acctcagagg tatccagcat gtgcacagat gctgcctccc aatctttccc
     1861 cacatgctcc atggaactat cattaccatt gtcctggaag tcccgatcac caggtgccat
     1921 atggccatga ctaccctcga gcagcctacc agcaagtgat ccagccggct ctgcctgggc
     1981 agcccctgcc tggagccagt gtgagaggcc tgcaccctgt gcagaaggtt atcctgaatt
     2041 atcccagccc ctgggaccaa gaagagaggc ccgcacagag agactgctcc tttccggggc
     2101 ttccaaggca ccaggaccag ccacatcacc agccacctaa tagagctggt gctcctgggg
     2161 agtccttgga gtgccctgca gagctgagac cacaggttcc ccagcctccg tccccagctg
     2221 ctgtgcctag accccctagc aaccctccag ccagaggaac tctaaaaaca agcaatttgc
     2281 cagaagaatt gcggaaagtc tttatcactt attcgatgga cacagctatg gaggtggtga
     2341 aattcgtgaa ctttttgttg gtaaatggct tccaaactgc aattgacata tttgaggata
     2401 gaatccgagg cattgatatc attaaatgga tggagcgcta ccttagggat aagaccgtga
     2461 tgataatcgt agcaatcagc cccaaataca aacaggacgt ggaaggcgct gagtcgcagc
     2521 tggacgagga tgagcatggc ttacatacta agtacattca tcgaatgatg cagattgagt
     2581 tcataaaaca aggaagcatg aatttcagat tcatccctgt gctcttccca aatgctaaga
     2641 aggagcatgt gcccacctgg cttcagaaca ctcatgtcta cagctggccc aagaataaaa
     2701 aaaacatcct gctgcggctg ctgagagagg aagagtatgt ggctcctcca cgggggcctc
     2761 tgcccaccct tcaggtggtt cccttggttt ctctcggctc gagcggcgtc ttcacactcg
     2821 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     2881 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     2941 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     3001 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     3061 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     3121 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     3181 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     3241 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     3301 gcgaacgcat tctggcgtaa ggccgcgact ctagagtcga cctgcaggca tgcaagctga
     3361 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     3421 actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac attgatgagt
     3481 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3541 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3601 ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc
     3661 tctacaaatg tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     3721 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc
     3781 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
     3841 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3961 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4021 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4081 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4141 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaattaact
     4201 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     4261 taaacccagg aggcagatca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4321 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4381 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4441 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4501 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4561 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4621 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4681 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4741 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4801 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4861 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4921 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4981 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5041 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5101 gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt
     5161 acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatcctct ttgcgcttgc
     5221 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
     5281 cggactggct ttctacccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5341 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5401 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5461 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5521 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5581 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5641 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5761 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5821 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5881 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5941 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6001 aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6061 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6121 tttatagtcc ggaaatacag gaacgcacgc tggatggccc ttcgctggga tggtgaaacc
     6181 atgaaaaatg gcagcttcag tggattaagt gggggtaatg tggcctgtac cctctggttg
     6241 cataggtatt catacggtta aaatttatca ggcgcgattg cggcagtttt tcgggtggtt
     6301 tgttgccatt tttacctgtc tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta
     6361 caattaaggg attatggtaa atccacttac tgtctgccct cgtagccatc gagataaacc
     6421 gcagtactcc ggccacgatg cgtccggcgt agaggatcga gatct
//
