LOCUS       Exported                6491 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..3320
                     /label=ACT1
                     /note="ACT1"
     misc_feature    3321..3328
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    3480..3701
                     /label=Sv40 late polyadenylation signal
     promoter        3800..4218
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4226..4292
                     /label=EM7 bacterial promoter
     CDS             4306..5100
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5164..5212
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5448..5484
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gcctcctcag cttcaagaaa
     1621 ctagaatgaa ccgaagcatt cctgtggagg ttgatgaatc agaaccatac ccaagtcagt
     1681 tgctgaaacc aatcccagaa tattccccgg aagaggaatc agaaccacct gctccaaata
     1741 taaggaacat ggcacccaac agcttgtctg cacccacaat gcttcacaat tcctccggag
     1801 acttttctca agctcactca accctgaaac ttgcaaatca ccagcggcct gtatcccggc
     1861 aggtcacctg cctgcgcact caagttctgg aggacagtga agacagtttc tgcaggagac
     1921 acccaggcct gggcaaagct ttcccttctg ggtgctctgc agtcagcgag cctgcgtctg
     1981 agtctgtggt tggagccctc cctgcagagc atcagttttc atttatggaa aaacgtaatc
     2041 aatggctggt atctcagctt tcagcggctt ctcctgacac tggccatgac tcagacaaat
     2101 cagaccaaag tttacctaat gcctcagcag actccttggg cggtagccag gagatggtgc
     2161 aacggcccca gcctcacagg aaccgagcag gcctggatct gccaaccata gacacgggat
     2221 atgattccca gccccaggat gtcctgggca tcaggcagct ggaaaggccc ctgcccctca
     2281 cctccgtgtg ttacccccag gacctcccca gacctctcag gtccagggag ttccctcagt
     2341 ttgaacctca gaggtatcca gcatgtgcac agatgctgcc tcccaatctt tccccacatg
     2401 ctccatggaa ctatcattac cattgtcctg gaagtcccga tcaccaggtg ccatatggcc
     2461 atgactaccc tcgagcagcc taccagcaag tgatccagcc ggctctgcct gggcagcccc
     2521 tgcctggagc cagtgtgaga ggcctgcacc ctgtgcagaa ggttatcctg aattatccca
     2581 gcccctggga ccaagaagag aggcccgcac agagagactg ctcctttccg gggcttccaa
     2641 ggcaccagga ccagccacat caccagccac ctaatagagc tggtgctcct ggggagtcct
     2701 tggagtgccc tgcagagctg agaccacagg ttccccagcc tccgtcccca gctgctgtgc
     2761 ctagaccccc tagcaaccct ccagccagag gaactctaaa aacaagcaat ttgccagaag
     2821 aattgcggaa agtctttatc acttattcga tggacacagc tatggaggtg gtgaaattcg
     2881 tgaacttttt gttggtaaat ggcttccaaa ctgcaattga catatttgag gatagaatcc
     2941 gaggcattga tatcattaaa tggatggagc gctaccttag ggataagacc gtgatgataa
     3001 tcgtagcaat cagccccaaa tacaaacagg acgtggaagg cgctgagtcg cagctggacg
     3061 aggatgagca tggcttacat actaagtaca ttcatcgaat gatgcagatt gagttcataa
     3121 aacaaggaag catgaatttc agattcatcc ctgtgctctt cccaaatgct aagaaggagc
     3181 atgtgcccac ctggcttcag aacactcatg tctacagctg gcccaagaat aaaaaaaaca
     3241 tcctgctgcg gctgctgaga gaggaagagt atgtggctcc tccacggggg cctctgccca
     3301 cccttcaggt ggttcccttg gtttaaacga attcgggctc ggtacccggg gatcctctag
     3361 agtcgacctg caggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag
     3421 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc
     3481 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3541 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3601 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     3661 ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa
     3721 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3781 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     3841 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3901 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3961 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4021 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4081 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4141 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4201 ggcttttgca aaaagcttaa ttaactgttg acaattaatc atcggcatag tatatcggca
     4261 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     4321 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4381 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4441 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4501 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4561 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4621 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4681 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4741 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4801 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4861 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4921 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4981 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5041 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5101 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc
     5161 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     5221 gcgataagga tcctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     5281 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acccggtatc agctcactca
     5341 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5401 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5461 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5521 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5581 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5641 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5701 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5761 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5821 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5881 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5941 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6001 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     6061 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6121 tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac gcacgctgga
     6181 tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga ttaagtgggg
     6241 gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat ttatcaggcg
     6301 cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg
     6361 cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc
     6421 tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc cggcgtagag
     6481 gatcgagatc t
//
