LOCUS       Exported                5471 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pRSG193 designed by Brock F. Binkowski

            

            PCR amplify pF-BiBiTLn w/ BFBRSG11/BFBRSG4 (PCR #1; 2.0 kbp);

            PCR amplify pF-BiBiTsmC w/ BFBRSG5/BFBRSG9 (PCR #2; 2.8 kbp); 

            PCR amplify pF-4B w/ BFBRSG10/BFBRSG12 (464 bp);

            3X component Gibson Assembly;

            electroporation of BR610.
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     gene            complement(407..529)
                     /label=HHR23A UBA1
     misc_feature    complement(530..550)
                     /label=Geneious type: polylinker
                     /note="Geneious type: polylinker"
     gene            complement(551..673)
                     /label=HHR23A UBA1
     misc_feature    complement(674..694)
                     /label=Geneious type: polylinker
                     /note="Geneious type: polylinker"
     gene            complement(695..817)
                     /label=HHR23A UBA1
     misc_feature    complement(818..838)
                     /label=Geneious type: polylinker
                     /note="Geneious type: polylinker"
     gene            complement(839..961)
                     /label=HHR23A UBA1
     misc_feature    complement(962..1009)
                     /label=Geneious type: polylinker
                     /note="Geneious type: polylinker"
     gene            complement(1010..1486)
                     /label=LgBiT
                     /note="LgBiT"
     misc_feature    1500..1526
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(1527..1543)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(1546..1591)
                     /label=Minimal CMV Promoter
     misc_feature    1569..1591
                     /label=core
                     /note="core"
     promoter        1601..2155
                     /label=CMV Enhancer
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2173..2239
                     /label=EM7 bacterial promoter
     misc_feature    2253..3047
                     /label=KanR
                     /note="KanR"
     rep_origin      3202..3238
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(3920..3967)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(3991..5028)
                     /label=HygroR
                     /note=" Feature found by back translation of amino acid 
                     feature definition."
     misc_feature    complement(5053..5471)
                     /label=SV40 promoter
                     /note="SV40 promoter"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ccccaagggg ttatgctagt tattgctcag cggtggcagc
      301 agccaactca gcttcctttc gggctttgtt agcagccgga tcagcttgca tgcctgcagg
      361 tcgactctag aggatccccg ggtaccgagc ccgaattcgt ttattagcct gtcagcaaat
      421 actcgacagc tcgatgagga ttgttatagg aggcccgcag ggcagctaca acgcgctcgc
      481 gttcgtaacc cattgacatt atctcagtaa gcatagtctc gtattcggat ccgctgccac
      541 ttccgctccc accggtcagc aggtattcaa ccgccctatg cggattatta tatgaggctc
      601 gcagcgcagc gaccactctc tccctctcgt atcccatact cataatctcg gtgagcatcg
      661 tctcatattc actgccgcta cctgatccac taccacccgt cagcagatac tcaacagctc
      721 tatgggggtt attataggaa gctcggagcg ctgcgacaac tcgttcccgt tcatagccca
      781 tggacataat ctctgtcagc atggtctcat actctgaacc tgacccgctt ccactccctc
      841 ctgtcagaag atattccacc gctcggtgag gattgttgta gctcgcgcgc agagccgcca
      901 caacgcgctc ccgttcatat cccattgaca ttatttccgt gagcatggtt tcatattcgg
      961 agccagaacc tcctcccccg gatcctcctc cgccggaccc tccaccacca ctgttgatgg
     1021 ttactcggaa cagcatggag ccgtcggggg tgatcaggcg ctcgtcgata attttgttgc
     1081 cgttccacag ggtccctgtt acagtgatct ttttgccgtc gaacacggcg atgccttcat
     1141 acggccgtcc gaaatagttc agcatgttcg gcgtaacccc gtcgattacc agtgtgccat
     1201 agggcaggat caccttaaag tgatgatcat ccacagggta caccacctta aacacctctt
     1261 cgatctgggc catttggtcg gcgctcagac cttcatacgg gatgatgaca tggatgtcga
     1321 tcttcagggc attttcaccg ctccggacaa tcctttggat cggagttacg gacacggcga
     1381 gattctgcag caaactggac acacctccct gttcaaggac ttggtccagg ttgtaggcgg
     1441 ctgtctgttc ccagtcccca acgaaatctt cgagtgtgaa gaccatggtg agcgctagct
     1501 gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatgggcgat ctgacggttc
     1561 actaaaccag ctctgcttat ataggcctcc ctggcgcgcc actagttatt aatagtaatc
     1621 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1681 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1741 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1801 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     1861 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     1921 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     1981 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2041 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2101 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtcaaatc
     2161 gaattctaat actgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     2221 caaggtgagg aactaaaccc aggaggcaga tcatgattga acaagatgga ttgcacgcag
     2281 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2341 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2401 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     2461 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2521 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2581 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2641 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2701 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2761 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     2821 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     2881 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     2941 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3001 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgattc gaaatgaccg
     3061 accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3121 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3181 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3241 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3301 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3361 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3421 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3481 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3541 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3601 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3661 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3721 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3781 aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3901 tttatagtcc ggaatcgtca cacaaaaaac caacacacag atgtaatgaa aataaagata
     3961 ttttattgcg gccatcgtga tggctagagt ctaaactcga cctacctcct tagcgcgcgg
     4021 tcgtgtactg ggccggcggt tgccgctgtc ggccagcacc tcgacgcagc cgtcggtcca
     4081 tacggctgcg ctccggcgag cgatttgtgt gcgaccgacg gtgccggccc cgctgcggac
     4141 gatggcatcg cagcggcctt gagcccaggc agcatcgtcg aagttgccgt ccacgaggct
     4201 ctggtagagc tgatccaggc cgatgcgcag catgtaggct cgcagacgag ggctgccggc
     4261 cagctcggga tgccggcgct cgaagtagcg agtctgctgc tccatgcaag ccagccaggg
     4321 ccgccagaag aagatgttgg ccacctcgta ctgactgtcc ccgaacatag cttcggacca
     4381 gtcgattacg gcggtgatgc ggccgttgtc ggtcaggacg ttgttgctgc cgaagtcggc
     4441 atggacgagg tggcgcacct cgggacagtc ttcggcccac agcatcagtt cgtccagggc
     4501 ttgagctacg ctggcggaca cggtgtcgtc catcacggtc tgccagtggt agacatgggg
     4561 atcagcaatg gcgcaaatga aatcccgcca ggttgtgtac tggccgatgc cttggggccc
     4621 gaatgggccg aagccgctgg tttgactgag gtcggcggcg gcaatagcat ccatagcttc
     4681 ggcgacaggc tgtaacacag caggcagctc tgtttcgggg aggtcttgga gagtaacgcc
     4741 ttgggcgcgt ctactgatgc agtatgtcag gctctcgctg aactcgccga tgtccaacac
     4801 ttcggggatg ggtagtgcag cgctggcgaa gtggcggtac acgtagcggt ctttgtagaa
     4861 gccatcagcg cagctattca cccgcagtac atagccgcgt ccgccgacat cgaagctgaa
     4921 ggctcggctc tcttcgccct ccgacaactg catcaggtcg ctcacactgt cgaacttctc
     4981 gatgagaaat ttttcaacgc tggtagcggt gagttcgggc ttcttcatgg tggcgctagt
     5041 gtcagaagaa tcgagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     5101 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     5161 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     5221 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5281 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     5341 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     5401 gttctttccg cctcagaagg tacctaacca agttcctctt tcagaggtta tttcaggcca
     5461 tggtgctgcg c
//
