LOCUS       Exported                7986 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-4P      5337 bp    DNA   circular            
            7/25/2012

            DEFINITION  Current Name: pF-4P
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..4268
                     /label=PIK3CA
                     /note="This insert was automatically updated on 2/14/2007. 
                     Any NCBI Synonyms,  and the comparison to the accession 
                     number were all automatically updated from NCBI."
     misc_feature    2697..2699
                     /label=E545K
                     /note="E545K"
     misc_feature    4269..4276
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    4428..4649
                     /label=Sv40 late polyadenylation signal
     misc_feature    4748..5166
                     /label=SV40 promoter
                     /note="SV40 promoter"
     CDS             5191..5589
                     /label=BlastR
                     /note="BlastR"
     polyA_signal    5614..5662
                     /label=Synthetic PolyAdenylation Signal
     CDS             5923..6783
                     /label=AmpR
                     /note="Removed RE site from standard beta-lactamase gene"
     misc_feature    6886..7808
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    7063
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcct ccacgaccat
     1081 catcaggtga actgtggggc atccacttga tgcccccaag aatcctagta gaatgtttac
     1141 taccaaatgg aatgatagtg actttagaat gcctccgtga ggctacatta ataaccataa
     1201 agcatgaact atttaaagaa gcaagaaaat accccctcca tcaacttctt caagatgaat
     1261 cttcttacat tttcgtaagt gttactcaag aagcagaaag ggaagaattt tttgatgaaa
     1321 caagacgact ttgtgacctt cggctttttc aacccttttt aaaagtaatt gaaccagtag
     1381 gcaaccgtga agaaaagatc ctcaatcgag aaattggttt tgctatcggc atgccagtgt
     1441 gtgaatttga tatggttaaa gatccagaag tacaggactt ccgaagaaat attctgaacg
     1501 tttgtaaaga agctgtggat cttagggacc tcaattcacc tcatagtaga gcaatgtatg
     1561 tctatcctcc aaatgtagaa tcttcaccag aattgccaaa gcacatatat aataaattag
     1621 ataaagggca aataatagtg gtgatctggg taatagtttc tccaaataat gacaagcaga
     1681 agtatactct gaaaatcaac catgactgtg taccagaaca agtaattgct gaagcaatca
     1741 ggaaaaaaac tcgaagtatg ttgctatcct ctgaacaact aaaactctgt gttttagaat
     1801 atcagggcaa gtatatttta aaagtgtgtg gatgtgatga atacttccta gaaaaatatc
     1861 ctctgagtca gtataagtat ataagaagct gtataatgct tgggaggatg cccaatttga
     1921 tgttgatggc taaagaaagc ctttattctc aactgccaat ggactgtttt acaatgccat
     1981 cttattccag acgcatttcc acagctacac catatatgaa tggagaaaca tctacaaaat
     2041 ccctttgggt tataaatagt gcactcagaa taaaaattct ttgtgcaacc tacgtgaatg
     2101 taaatattcg agacattgat aagatctatg ttcgaacagg tatctaccat ggaggagaac
     2161 ccttatgtga caatgtgaac actcaaagag taccttgttc caatcccagg tggaatgaat
     2221 ggctgaatta tgatatatac attcctgatc ttcctcgtgc tgctcgactt tgcctttcca
     2281 tttgctctgt taaaggccga aagggtgcta aagaggaaca ctgtccattg gcatggggaa
     2341 atataaactt gtttgattac acagacactc tagtatctgg aaaaatggct ttgaatcttt
     2401 ggccagtacc tcatggatta gaagatttgc tgaaccctat tggtgttact ggatcaaatc
     2461 caaataaaga aactccatgc ttagagttgg agtttgactg gttcagcagt gtggtaaagt
     2521 tcccagatat gtcagtgatt gaagagcatg ccaattggtc tgtatcccga gaagcaggat
     2581 ttagctattc ccacgcagga ctgagtaaca gactagctag agacaatgaa ttaagggaaa
     2641 atgacaaaga acagctcaaa gcaatttcta cacgagatcc tctctctgaa atcactaaac
     2701 aggagaaaga ttttctatgg agtcacagac actattgtgt aactatcccc gaaattctac
     2761 ccaaattgct tctgtctgtt aaatggaatt ctagagatga agtagcccag atgtattgct
     2821 tggtaaaaga ttggcctcca atcaaacctg aacaggctat ggaacttctg gactgtaatt
     2881 acccagatcc tatggttcga ggttttgctg ttcggtgctt ggaaaaatat ttaacagatg
     2941 acaaactttc tcagtattta attcagctag tacaggtcct aaaatatgaa caatatttgg
     3001 ataacttgct tgtgagattt ttactgaaga aagcattgac taatcaaagg attgggcact
     3061 ttttcttttg gcatttaaaa tctgagatgc acaataaaac agttagccag aggtttggcc
     3121 tgcttttgga gtcctattgt cgtgcatgtg ggatgtattt gaagcacctg aataggcaag
     3181 tcgaggcaat ggaaaagctc attaacttaa ctgacattct caaacaggag aagaaggatg
     3241 aaacacaaaa ggtacagatg aagtttttag ttgagcaaat gaggcgacca gatttcatgg
     3301 atgctctaca gggctttctg tctcctctaa accctgctca tcaactagga aacctcaggc
     3361 ttgaagagtg tcgaattatg tcctctgcaa aaaggccact gtggttgaat tgggagaacc
     3421 cagacatcat gtcagagtta ctgtttcaga acaatgagat catctttaaa aatggggatg
     3481 atttacggca agatatgcta acacttcaaa ttattcgtat tatggaaaat atctggcaaa
     3541 atcaaggtct tgatcttcga atgttacctt atggttgtct gtcaatcggt gactgtgtgg
     3601 gacttattga ggtggtgcga aattctcaca ctattatgca aattcagtgc aaaggcggct
     3661 tgaaaggtgc actgcagttc aacagccaca cactacatca gtggctcaaa gacaagaaca
     3721 aaggagaaat atatgatgca gccattgacc tgtttacacg ttcatgtgct ggatactgtg
     3781 tagctacctt cattttggga attggagatc gtcacaatag taacatcatg gtgaaagacg
     3841 atggacaact gtttcatata gattttggac actttttgga tcacaagaag aaaaaatttg
     3901 gttataaacg agaacgtgtg ccatttgttt tgacacagga tttcttaata gtgattagta
     3961 aaggagccca agaatgcaca aagacaagag aatttgagag gtttcaggag atgtgttaca
     4021 aggcttatct agctattcga cagcatgcca atctcttcat aaatcttttc tcaatgatgc
     4081 ttggctctgg aatgccagaa ctacaatctt ttgatgacat tgcatacatt cgaaagaccc
     4141 tagccttaga taaaactgag caagaggctt tggagtattt catgaaacaa atgaatgatg
     4201 cacatcatgg tggctggaca acaaaaatgg attggatctt ccacacaatt aaacagcatg
     4261 cattgaacgt ttaaacgaat tcgggctcgg tacccgggga tcctctagag tcgacctgca
     4321 ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     4381 accgctgagc aataactagc ataacccctt ggggcggccg cttcgagcag acatgataag
     4441 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4501 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4561 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     4621 aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata ttaacgctta
     4681 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4741 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     4801 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4861 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4921 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5161 aagctcgatt cttctgacac tagcgccacc atggccaagc ctttgtctca agaagaatcc
     5221 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     5281 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     5341 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     5401 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     5461 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     5521 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     5581 gagggctaat gatctagaac cggtcatggc cgcaataaaa tatctttatt ttcattacat
     5641 ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tcctctttgc gcttgcgttt
     5701 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
     5761 ctggctttct acgtaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5821 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5881 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5941 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6001 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6061 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6121 atgagcactt tcaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6181 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6241 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6301 atgagtgata acactgcggc caacttactt ctgacaacta tcggaggacc gaaggagcta
     6361 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6421 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6481 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6541 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6601 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6661 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6721 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6781 taattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa
     6841 tacggttatc cacagaatca ggggataacg caggaaagaa catgtcaaac atgagaatta
     6901 caacttatat cgtatggggc tgacttcagg tgctacattt gaagagataa attgcactga
     6961 aatctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
     7021 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     7081 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     7141 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     7201 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     7261 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     7321 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     7381 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     7441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     7501 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg
     7561 gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
     7621 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
     7681 aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc
     7741 tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca
     7801 ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcatgagat
     7861 tacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc
     7921 tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg
     7981 agatct
//
