LOCUS       Exported                5821 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFC36K      4412 bp    DNA   circular            
            7/1/2019

            DEFINITION  Current Name: pFC36K
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..753
                     /label=HSV-TK promoter
     misc_feature    793..800
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    802..2586
                     /label=P2RX7
                     /note="P2RX7"
     misc_feature    2596..2640
                     /label=linker
                     /note="linker"
     misc_feature    2641..2673
                     /label=SmBiT
     polyA_signal    2810..3031
                     /label=Sv40 late polyadenylation signal
     promoter        3130..3548
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3556..3622
                     /label=EM7 bacterial promoter
     CDS             3636..4430
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4494..4542
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      4778..4814
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 aaatgagtct tcggacctcg cgggggccgc ttaagcggtg gttagggttt gtctgacgcg
       61 gggggagggg gaaggaacga aacactctca ttcggaggcg gctcggggtt tggtcttggt
      121 ggccacgggc acgcagaaga gcgccgcgat cctcttaagc acccccccgc cctccgtgga
      181 ggcgggggtt tggtcggcgg gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc
      241 cccagagtgt gaccttttcg gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg
      301 acgggctcgc tgggtcctag gctccatggg gaccgtatac gtggacaggc tctggagcat
      361 ccgcacgact gcggtgatat taccggagac cttctgcggg acgagccggg tcacgcggct
      421 gacgcggagc gtccgttggg cgacaaacac caggacgggg cacaggtaca ctatcttgtc
      481 acccggaggc gcgagggact gcaggagctt cagggagtgg cgcagctgct tcatccccgt
      541 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
      601 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaagtcga acacgcagat
      661 gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
      721 gaacaccgag cgaccctgca gcgacccgct taaaagcttg gcaatccggt actgttggta
      781 aagccaccgc tagcgatcgc catgccggcc tgctgcagct gcagtgatgt tttccagtat
      841 gagacgaaca aagtcactcg gatccagagc atgaattatg gcaccattaa gtggttcttc
      901 cacgtgatca tcttttccta cgtttgcttt gctctggtga gtgacaagct gtaccagcgg
      961 aaagagcctg tcatcagttc tgtgcacacc aaggtgaagg ggatagcaga ggtgaaagag
     1021 gagatcgtgg agaatggagt gaagaagttg gtgcacagtg tctttgacac cgcagactac
     1081 accttccctt tgcaggggaa ctctttcttc gtgatgacaa actttctcaa aacagaaggc
     1141 caagagcagc ggttgtgtcc cgagtatccc acccgcagga cgctctgttc ctctgaccga
     1201 ggttgtaaaa agggatggat ggacccgcag agcaaaggaa ttcagaccgg aaggtgtgta
     1261 gtgtatgaag ggaaccagaa gacctgtgaa gtctctgcct ggtgccccat cgaggcagtg
     1321 gaagaggccc cccggcctgc tctcttgaac agtgccgaaa acttcactgt gctcatcaag
     1381 aacaatatcg acttccccgg ccacaactac accacgagaa acatcctgcc aggtttaaac
     1441 atcacttgta ccttccacaa gactcagaat ccacagtgtc ccattttccg actaggagac
     1501 atcttccgag aaacaggcga taatttttca gatgtggcaa ttcagggcgg aataatgggc
     1561 attgagatct actgggactg caacctagac cgttggttcc atcactgccg tcccaaatac
     1621 agtttccgtc gccttgacga caagaccacc aacgtgtcct tgtaccctgg ctacaacttc
     1681 agatacgcca agtactacaa ggaaaacaat gttgagaaac ggactctgat aaaagtcttc
     1741 gggatccgtt ttgacatcct ggtttttggc accggaggaa aatttgacat tatccagctg
     1801 gttgtgtaca tcggctcaac cctctcctac ttcggtctgg ccgctgtgtt catcgacttc
     1861 ctcatcgaca cttactccag taactgctgt cgctcccata tttatccctg gtgcaagtgc
     1921 tgtcagccct gtgtggtcaa cgaatactac tacaggaaga agtgcgagtc cattgtggag
     1981 ccaaagccga cattaaagta tgtgtccttt gtggatgaat cccacattag gatggtgaac
     2041 cagcagctac tagggagaag tctgcaagat gtcaagggcc aagaagtccc aagacctgcg
     2101 atggacttca cagatttgtc caggctgccc ctggccctcc atgacacacc cccgattcct
     2161 ggacaaccag aggagataca gctgcttaga aaggaggcga ctcctagatc cagggatagc
     2221 cccgtctggt gccagtgtgg aagctgcctc ccatctcaac tccctgagag ccacaggtgc
     2281 ctggaggagc tgtgctgccg gaaaaagccg ggggcctgca tcaccacctc agagctgttc
     2341 aggaagctgg tcctgtccag acacgtcctg cagttcctcc tgctctacca ggagcccttg
     2401 ctggcgctgg atgtggattc caccaacagc cggctgcggc actgtgccta caggtgctac
     2461 gccacctggc gcttcggctc ccaggacatg gctgactttg ccatcctgcc cagctgctgc
     2521 cgctggagga tccggaaaga gtttccgaag agtgaagggc agtacagtgg cttcaagagt
     2581 ccttacgttt ctcaaggcag ttcaggtggt ggcgggagcg gaggtggagg ctcgagcggt
     2641 gtgaccggct accggctgtt cgaggagatt ctgtaaggcc gcgactctag agtcgacctg
     2701 caggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     2761 ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata
     2821 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2881 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2941 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     3001 ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct
     3061 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3121 cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta
     3181 ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3361 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3421 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3481 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3541 aaaagcttaa ttaactgttg acaattaatc atcggcatag tatatcggca tagtataata
     3601 cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat ggattgcacg
     3661 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3781 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3901 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3961 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4021 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4081 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4141 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     4201 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4261 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4321 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4381 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4441 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa
     4501 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga
     4561 tcctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     4621 gtcagcaccg tttctgcgga ctggctttct acccggtatc agctcactca aaggcggtaa
     4681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5221 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5341 agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg
     5401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5461 tcttcaccta gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg
     5521 ctgggatggt gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc
     5581 ctgtaccctc tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc
     5641 agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
     5701 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
     5761 gccatcgaga taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc
     5821 t
//
