LOCUS       Exported                8168 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(278..787)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    complement(788..802)
                     /label=linker
                     /note="linker"
     misc_feature    complement(802..804)
                     /label=EcoICR I
     misc_feature    complement(805..808)
                     /label=PmeI
                     /note="PmeI"
     misc_feature    complement(809..3433)
                     /label=UBE3A (E6AP)
                     /note=" UBE3A (E6AP)"
     misc_feature    complement(3435..3442)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3447..3473
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(3474..3490)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(3493..3538)
                     /label=Minimal CMV Promoter 2
     misc_feature    3516..3538
                     /label=core
                     /note="core"
     promoter        3548..4102
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        4120..4186
                     /label=EM7 bacterial promoter
     CDS             4200..4859
                     /label=Chloramphenicol resistance
     rep_origin      5028..5064
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5746..5793)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(5817..6611)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(6625..6919)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(6990..8168)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagagtcgc ggccttacgc cagaatgcgt tcgcacagcc
      301 gccagccggt cactccgttg atggttactc ggaacagcag ggagccgtcg gggttgatca
      361 ggcgctcgtc gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc
      421 cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa
      481 ccccgtcgat taccagtgtg ccatagtgca ggatcacctt aaagtgatga tcatccacag
      541 ggtacaccac cttaaaaatt ttttcgatct ggcccatttg gtcgccgctc agaccttcat
      601 acgggatgat gacatggatg tcgatcttca gcccattttc accgctcagg acaatccttt
      661 ggatcggagt tacggacacc ccgagattct gaaacaaact ggacacacct ccctgttcaa
      721 ggacttggtc caggttgtag ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg
      781 tgaagacgcc gctcgagccg agagaaacca gcatgccaaa tcctttggca tacgtgatgg
      841 ccttcaacaa tctctcttta agtttttctt tgcttgagta ttccggaagt aaaagcacat
      901 taaagcaagt atgagatgta ggtaaccttt ctgtgtctgg gccatttttg gctataatca
      961 tctttaattt tcctagtcct cccacaggtg ctctgtctgt gcccgttgta aactgcaaga
     1021 agagtctttt ctgttcatct gtaaatgaat gaacgatttc ccagaactcc ctaatcagaa
     1081 cagagtccct ggtatagcca ccgtcatatt ctgtagtttc ttctagtgct tggaaatcta
     1141 gattccggct tccacatata agcaattcaa tttcttctgg tctgaataag tactttaagg
     1201 gagattcatt ggtcaccata tgaaaacctc tccgaaaagc cttgaactgt ttttctactg
     1261 atttattgag aatgtagtca gaataaagat tgacaaattc cttcctgttt tcatttgtaa
     1321 ttggaatttt atcaccattt tcctttagat catacatcat tgggttacca aaaagatctg
     1381 tctgtgatat ctggaaagtg atcatcatgt catcttccac attcccttca tactccaata
     1441 aatcttttaa actctgatat agaactgggt gagagtctcc caagtcacga aaagttcctt
     1501 ttttccccat tagcttcctg tagacaacca tgggaaaatg tacatccagt atacagttat
     1561 tgtaaatagc cagacccagt actatgccaa tcagagtaaa ctgaccctca gtttcaaaag
     1621 aagatggatt aaaccaaaac aattttgtag attcatcgta tgtgaacata ccaatatctg
     1681 gattgaagat ttcctccaca accagctgaa aaaattcttt ggaaacacct ccctcatcaa
     1741 ctccttgttc tccttcaaat tccacataca actgcttctt caagtctgca ggattttcca
     1801 tagcgatcat ctctagccgg acaagtgcat catctatgat atggtcacgt ctaactttga
     1861 gtctcaaata tggattcaac tgctgtcctt gaactaagct gtagagaaca gtgattcttc
     1921 gttcactgta catgcgaatt ctattgtcat aatataatcc caaattcttt gtgacagcat
     1981 tcaatataaa gggacatgtc ataaaagaga atttgttctc tgtttctact ttgaaaaaag
     2041 tataatcttt atccatttct agaacctcat tcagtggttc attaataaac tcttcaaaag
     2101 ggataagtgg ttttcgacaa tccagggttt taacaccaag ttcagtttcc agggggtcca
     2161 ctcgaggacc tttcttgttt cttctttctt ctcccaaaag ttcctgaagt gtcagctcgc
     2221 tggactcagg gatgggctct tcatcatctt cttcattgtg atttgtgtcc acttcccctc
     2281 ccactacatt tgcatagtaa accattttca agcacttcga agcagcaaca atggcatcat
     2341 catcattcac tagatttcga ctgttaaatt cattgcttat gactttataa gtaataagtt
     2401 gctgaaatgt ctccatcatt ctccgaatct ggtctgcatt gtatttagac cacagtctga
     2461 tcagttttcc ttgggctgca aggggtagct tgctcatcgc tttgcaaaat aatggcaaag
     2521 ccatttccag atattcagga ctgtggagat ttctattctc cattacgata atgaacaaat
     2581 tcagataatt aggatctcga gagtatacat tgtgatacgt caagtcacat tccacgttag
     2641 gtgacaaata tacaagtgca ttgagaaagg cagtttcaat tttttcatta gagagcaatc
     2701 tggtgtagac ccttctaatg gcatcaatat ccacagacac atcatcaggg cctaattttt
     2761 gcaaattgtt gtctccctgt gagctatcac ctatccttga ggaagatgct tctgagtctt
     2821 cttccatagc agcagcagaa catgcagctt tttccttttc atcttcatct ttgtcttcat
     2881 cttttgcttg aagagatttc agttcttcct tggtgtgttg tttaactttc cggaagctct
     2941 gtaccaatgc ctcagcacta gaaaaaactc ttccaataac acggattaaa ggggaataat
     3001 cctctctttc tctacataat tcaagaattt catatacctt ctcttctgtt aagtaagtca
     3061 catctttaaa atcaattcta gcgcctttct tgttcatttt tatctcagag caggagttgt
     3121 tgggggcacc tttcgagttc tcaaggtaag ctgagcttgc tcctttcttg gagggatgag
     3181 gatcacagag ttttgcatta atcttataaa gctcgagggc tttaatagct gctgcattat
     3241 tatccatacg aagaaaagtt ggacaggaag cacaaaactc attcgtgcag gcttcatttc
     3301 cacagccctc agttaactgg tggtagtagc gttctattag atgctttgca gctgctcgct
     3361 tcattcggct agcttcaatg tcgtcagact gaggttctcc tgatttccaa taacactggt
     3421 gcagcttctc catggcgatc gctagctgca aaaagaacaa gtagcttgta ttctatagtg
     3481 tcacctaaat gggcgatctg acggttcact aaaccagctc tgcttatata ggcctccctg
     3541 gcgcgccact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3601 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3661 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3721 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3781 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3841 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     3901 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     3961 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     4021 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     4081 taggcgtgta cggtgggagg tcaaatcgaa ttctaatact gttgacaatt aatcatcggc
     4141 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca
     4201 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     4261 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     4321 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     4381 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     4441 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     4501 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     4561 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     4621 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     4681 ccaatatgga caacttcttc gcccccgttt tcactatggg caaatattat acgcaaggcg
     4741 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
     4801 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaag
     4861 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     4921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5461 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5521 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5581 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     5641 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5701 tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac
     5761 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagttcag
     5821 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5881 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     5941 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     6001 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccgtg ggtcacgacg
     6061 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     6121 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6181 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6241 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6301 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6361 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6421 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6481 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6541 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6601 tgttcaatca tggtggcatc gattcccggt caacatccag tacacctccg gcaccaccgg
     6661 cttccccaag ggcgccacgc tctcccacca caacatcctc aacaacggct actgggtggg
     6721 ccgcacggtc ggctacacgg agcaggaccg ggtgtgtctg ccggtgccct tctaccactg
     6781 cttcggcatg gtcatgggca acttgggcgc cacctcccac gccgcctgca tcgtcatccc
     6841 ggccccgtcc ttcgagccgg cggcgacgct ggaggcggtg cagcgggaga ggtgcacgtc
     6901 cctgtacggc gtccccacga agccgaattc caccacactg gactagatgc atgctcgaca
     6961 gtgtgatgga tatctgcaga attcggcttt cacgacacct gaaatggaag aaaaaaactt
     7021 tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc
     7081 aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg
     7141 ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc
     7201 gagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg
     7261 gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag
     7321 cgccgcgtcc tccattttga gctgcctgca gcagggccgg gaagcggcca tctttccgct
     7381 cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc
     7441 gcgaggccag gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt
     7501 ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg
     7561 ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc atttacaaga
     7621 ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat ggctagagac
     7681 ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag
     7741 cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc
     7801 gaactctccc acccacttcc aacccgaagc tcgggatcaa gaatcacgta ctgcagccag
     7861 gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa agaggccagg cccgcgggaa
     7921 ccacacacgg cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg
     7981 gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg gagccagtac
     8041 acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt tcaattgccg
     8101 acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca ctgacgggca
     8161 ccggagcc
//
