LOCUS       Exported               16676 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)-MLL(V1)      16676 bp    DNA   circular
                       10/17/2016

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) - MLL(V1) Old/Alternate 
            Name(s):ALL-1 (insert) CXXC7 (insert) FLJ11783 (insert) HRX (insert)
            HTRX1 (insert) KMT2A (insert) MLL (insert) MLL(V1) (insert) MLL/GAS7
            (insert) MLL1A (insert) TET1-MLL (insert) TRX1 (insert) pF-Nn5K3 
            (backbone)
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     misc_feature    992..997
                     /label=6base
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     CDS             1065..13511
                     /label=Complete ORF (with fusions) of MLL(V1)
     CDS             1065..1577
                     /label=NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1594..13509
                     /label=MLL(V1) insert
     misc_feature    complement(3452..3457)
                     /label=6base
     misc_feature    3691..3696
                     /label=EcoICR I
     misc_feature    3894..3899
                     /label=EcoICR I
     misc_feature    complement(5310..5315)
                     /label=6base
     misc_feature    5790..5795
                     /label=6base
     misc_feature    complement(5805..5810)
                     /label=6base
     misc_feature    complement(7289..7294)
                     /label=6base
     misc_feature    complement(7593..7598)
                     /label=6base
     misc_feature    7625..7631
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    8495..8500
                     /label=EcoICR I
     misc_feature    8540..8545
                     /label=EcoICR I
     misc_feature    10026..10031
                     /label=6base
     misc_feature    10077..10082
                     /label=EcoICR I
     misc_feature    complement(10824..10830)
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    12900..12905
                     /label=6base
     misc_feature    complement(13136..13142)
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    13506..13513
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    13510..13643
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    13665..13886
                     /label=Sv40 late polyadenylation signal
     promoter        13985..14403
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(14058..14294)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      14301..14366
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        14411..14477
                     /label=EM7 bacterial promoter
     CDS             14491..15285
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    15349..15397
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      15633..15669
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    16340..16625
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggcgcacagc tgtcggtggc
     1621 gcttccccgc ccgacccggg accaccgggg gcggcggcgg cggggggcgc cggggcctag
     1681 ggggcgcccc gcggcaacgc gtcccggccc tgctgcttcc ccccgggccc ccggtcggcg
     1741 gtggcggccc cggggcgccc ccctcccccc cggctgtggc ggccgcggcg gcggcggcgg
     1801 gaagcagcgg ggctggggtt ccagggggag cggccgccgc ctcagcagcc tcctcgtcgt
     1861 ccgcctcgtc ttcgtcttcg tcatcgtcct cagcctcttc agggccggcc ctgctccggg
     1921 tgggcccggg cttcgacgcg gcgctgcagg tctcggccgc catcggcacc aacctgcgcc
     1981 ggttccgggc cgtgtttggg gagagcggcg ggggaggcgg cagcggagag gatgagcaat
     2041 tcttaggttt tggctcagat gaagaagtca gagtgcgaag tcccacaagg tctccttcag
     2101 ttaaaactag tcctcgaaaa cctcgtggga gacctagaag tggctctgac cgaaattcag
     2161 ctatcctctc agatccatct gtgttttccc ctctaaataa atcagagacc aaatctggag
     2221 ataagatcaa gaagaaagat tctaaaagta tagaaaagaa gagaggaaga cctcccacct
     2281 tccctggagt aaaaatcaaa ataacacatg gaaaggacat ttcagagtta ccaaagggaa
     2341 acaaagaaga tagcctgaaa aaaattaaaa ggacaccttc tgctacgttt cagcaagcca
     2401 caaagattaa aaaattaaga gcaggtaaac tctctcctct caagtctaag tttaagacag
     2461 ggaagcttca aataggaagg aagggggtac aaattgtacg acggagagga aggcctccat
     2521 caacagaaag gataaagacc ccttcgggtc tcctcattaa ttctgaactg gaaaagcccc
     2581 agaaagtccg gaaagacaag gaaggaacac ctccacttac aaaagaagat aagacagttg
     2641 tcagacaaag ccctcgaagg attaagccag ttaggattat tccttcttca aaaaggacag
     2701 atgcaaccat tgctaagcaa ctcttacaga gggcaaaaaa gggggctcaa aagaaaattg
     2761 aaaaagaagc agctcagctg cagggaagaa aggtgaagac acaggtcaaa aatattcgac
     2821 agttcatcat gcctgttgtc agtgctatct cctcgcggat cattaagacc cctcggcggt
     2881 ttatagagga tgaggattat gaccctccaa ttaaaattgc ccgattagag tctacaccga
     2941 atagtagatt cagtgccccg tcctgtggat cttctgaaaa atcaagtgca gcttctcagc
     3001 actcctctca aatgtcttca gactcctctc gatctagtag ccccagtgtt gatacctcca
     3061 cagactctca ggcttctgag gagattcagg tacttcctga ggagcggagc gatacccctg
     3121 aagttcatcc tccactgccc atttcccagt ccccagaaaa tgagagtaat gataggagaa
     3181 gcagaaggta ttcagtgtcg gagagaagtt ttggatctag aacgacgaaa aaattatcaa
     3241 ctctacaaag tgccccccag cagcagacct cctcgtctcc acctccacct ctgctgactc
     3301 caccgccacc actgcagcca gcctccagta tctctgacca cacaccttgg cttatgcctc
     3361 caacaatccc cttagcatca ccatttttgc ctgcttccac tgctcctatg caagggaagc
     3421 gaaaatctat tttgcgagaa ccgacattta ggtggacttc tttaaagcat tctaggtcag
     3481 agccacaata cttttcctca gcaaagtatg ccaaagaagg tcttattcgc aaaccaatat
     3541 ttgataattt ccgaccccct ccactaactc ccgaggacgt tggctttgca tctggttttt
     3601 ctgcatctgg taccgctgct tcagcccgat tgttttcgcc actccattct ggaacaaggt
     3661 ttgatatgca caaaaggagc cctcttctga gagctccaag atttactcca agtgaggctc
     3721 actctagaat atttgagtct gtaaccttgc ctagtaatcg aacttctgct ggaacatctt
     3781 cttcaggagt atccaataga aaaaggaaaa gaaaagtgtt tagtcctatt cgatctgaac
     3841 caagatctcc ttctcactcc atgaggacaa gaagtggaag gcttagtagt tctgagctct
     3901 cacctctcac ccccccgtct tctgtctctt cctcgttaag catttctgtt agtcctcttg
     3961 ccactagtgc cttaaaccca acttttactt ttccttctca ttccctgact cagtctgggg
     4021 aatctgcaga gaaaaatcag agaccaagga agcagactag tgctccggca gagccatttt
     4081 catcaagtag tcctactcct ctcttccctt ggtttacccc aggctctcag actgaaagag
     4141 ggagaaataa agacaaggcc cccgaggagc tgtccaaaga tcgagatgct gacaagagcg
     4201 tggagaagga caagagtaga gagagagacc gggagagaga aaaggagaat aagcgggagt
     4261 caaggaaaga gaaaaggaaa aagggatcag aaattcagag tagttctgct ttgtatcctg
     4321 tgggtagggt ttccaaagag aaggttgttg gtgaagatgt tgccacttca tcttctgcca
     4381 aaaaagcaac agggcggaag aagtcttcat cacatgattc tgggactgat attacttctg
     4441 tgactcttgg ggatacaaca gctgtcaaaa ccaaaatact tataaagaaa gggagaggaa
     4501 atctggaaaa aaccaacttg gacctcggcc caactgcccc atccctggag aaggagaaaa
     4561 ccctctgcct ttccactcct tcatctagca ctgttaaaca ttccacttcc tccataggct
     4621 ccatgttggc tcaggcagac aagcttccaa tgactgacaa gagggttgcc agcctcctaa
     4681 aaaaggccaa agctcagctc tgcaagattg agaagagtaa gagtcttaaa caaaccgacc
     4741 agcccaaagc acagggtcaa gaaagtgact catcagagac ctctgtgcga ggaccccgga
     4801 ttaaacatgt ctgcagaaga gcagctgttg cccttggccg aaaacgagct gtgtttcctg
     4861 atgacatgcc caccctgagt gccttaccat gggaagaacg agaaaagatt ttgtcttcca
     4921 tggggaatga tgacaagtca tcaattgctg gctcagaaga tgctgaacct cttgctccac
     4981 ccatcaaacc aattaaacct gtcactagaa acaaggcacc ccaggaacct ccagtaaaga
     5041 aaggacgtcg atcgaggcgg tgtgggcagt gtcccggctg ccaggtgcct gaggactgtg
     5101 gtgtttgtac taattgctta gataagccca agtttggtgg tcgcaatata aagaagcagt
     5161 gctgcaagat gagaaaatgt cagaatctac aatggatgcc ttccaaagcc tacctgcaga
     5221 agcaagctaa agctgtgaaa aagaaagaga aaaagtctaa gaccagtgaa aagaaagaca
     5281 gcaaagagag cagtgttgtg aagaacgtgg tggactctag tcagaaacct accccatcag
     5341 caagagagga tcctgcccca aagaaaagca gtagtgagcc tcctccacga aagcccgtcg
     5401 aggaaaagag tgaagaaggg aatgtctcgg cccctgggcc tgaatccaaa caggccacca
     5461 ctccagcttc caggaagtca agcaagcagg tctcccagcc agcactggtc atcccgcctc
     5521 agccacctac tacaggaccg ccaagaaaag aagttcccaa aaccactcct agtgagccca
     5581 agaaaaagca gcctccacca ccagaatcag gtccagagca gagcaaacag aaaaaagtgg
     5641 ctccccgccc aagtatccct gtaaaacaaa aaccaaaaga aaaggaaaaa ccacctccgg
     5701 tcaataagca ggagaatgca ggcactttga acatcctcag cactctctcc aatggcaata
     5761 gttctaagca aaaaattcca gcagatggag tccacaggat cagagtggac tttaaggagg
     5821 attgtgaagc agaaaatgtg tgggagatgg gaggcttagg aatcttgact tctgttccta
     5881 taacacccag ggtggtttgc tttctctgtg ccagtagtgg gcatgtagag tttgtgtatt
     5941 gccaagtctg ttgtgagccc ttccacaagt tttgtttaga ggagaacgag cgccctctgg
     6001 aggaccagct ggaaaattgg tgttgtcgtc gttgcaaatt ctgtcacgtt tgtggaaggc
     6061 aacatcaggc tacaaagcag ctgctggagt gtaataagtg ccgaaacagc tatcaccctg
     6121 agtgcctggg accaaactac cccaccaaac ccacaaagaa gaagaaagtc tggatctgta
     6181 ccaagtgtgt tcgctgtaag agctgtggat ccacaactcc aggcaaaggg tgggatgcac
     6241 agtggtctca tgatttctca ctgtgtcatg attgcgccaa gctctttgct aaaggaaact
     6301 tctgccctct ctgtgacaaa tgttatgatg atgatgacta tgagagtaag atgatgcaat
     6361 gtggaaagtg tgatcgctgg gtccattcca aatgtgagaa tctttcagat gagatgtatg
     6421 agattctatc taatctgcca gaaagtgtgg cctacacttg tgtgaactgt actgagcggc
     6481 accctgcaga gtggcgactg gcccttgaaa aagagctgca gatttctctg aagcaagttc
     6541 tgacagcttt gttgaattct cggactacca gccatttgct acgctaccgg caggctgcca
     6601 agcctccaga cttaaatccc gagacagagg agagtatacc ttcccgcagc tcccccgaag
     6661 gacctgatcc accagttctt actgaggtca gcaaacagga tgatcagcag cctttagatc
     6721 tagaaggagt caagaggaag atggaccaag ggaattacac atctgtgttg gagttcagtg
     6781 atgatattgt gaagatcatt caagcagcca ttaattcaga tggaggacag ccagaaatta
     6841 aaaaagccaa cagcatggtc aagtccttct tcattcggca aatggaacgt gtttttccat
     6901 ggttcagtgt caaaaagtcc aggttttggg agccaaataa agtatcaagc aacagtggga
     6961 tgttaccaaa cgcagtgctt ccaccttcac ttgaccataa ttatgctcag tggcaggagc
     7021 gagaggaaaa cagccacact gagcagcctc ctttaatgaa gaaaatcatt ccagctccca
     7081 aacccaaagg tcctggagaa ccagactcac caactcctct gcatcctcct acaccaccaa
     7141 ttttgagtac tgataggagt cgagaagaca gtccagagct gaacccaccc ccaggcatag
     7201 aagacaatag acagtgtgcg ttatgtttga cttatggtga tgacagtgct aatgatgctg
     7261 gtcgtttact atatattggc caaaatgagt ggacacatgt aaattgtgct ttgtggtcag
     7321 cggaagtgtt tgaagatgat gacggatcac taaagaatgt gcatatggct gtgatcaggg
     7381 gcaagcagct gagatgtgaa ttctgccaaa agccaggagc caccgtgggt tgctgtctca
     7441 catcctgcac cagcaactat cacttcatgt gttcccgagc caagaactgt gtctttctgg
     7501 atgataaaaa agtatattgc caacgacatc gggatttgat caaaggcgaa gtggttcctg
     7561 agaatggatt tgaagttttc agaagagtgt ttgtggactt tgaaggaatc agcttgagaa
     7621 ggaagtttct caatggcttg gaaccagaaa atatccacat gatgattggg tctatgacaa
     7681 tcgactgctt aggaattcta aatgatctct ccgactgtga agataagctc tttcctattg
     7741 gatatcagtg ttccagggta tactggagca ccacagatgc tcgcaagcgc tgtgtatata
     7801 catgcaagat agtggagtgc cgtcctccag tcgtagagcc ggatatcaac agcactgttg
     7861 aacatgatga aaacaggacc attgcccata gtccaacatc ttttacagaa agttcatcaa
     7921 aagagagtca aaacacagct gaaattataa gtcctccatc accagaccga cctcctcatt
     7981 cacaaacctc tggctcctgt tattatcatg tcatctcaaa ggtccccagg attcgaacac
     8041 ccagttattc tccaacacag agatcccctg gctgtcgacc gttgccttct gcaggaagtc
     8101 ctaccccaac cactcatgaa atagtcacag taggtgatcc tttactctcc tctggacttc
     8161 gaagcattgg ctccaggcgt cacagtacct cttccttatc accccagcgg tccaaactcc
     8221 ggataatgtc tccaatgaga actgggaata cttactctag gaataatgtt tcctcagtct
     8281 ccaccaccgg gaccgctact gatcttgaat caagtgccaa agtagttgat catgtcttag
     8341 ggccactgaa ttcaagtact agtttagggc aaaacacttc cacctcttca aatttgcaaa
     8401 ggacagtggt tactgtaggc aataaaaaca gtcacttgga tggatcttca tcttcagaaa
     8461 tgaagcagtc cagtgcttca gacttggtgt ccaagagctc ctctttaaag ggagagaaga
     8521 ccaaagtgct gagttccaag agctcagagg gatctgcaca taatgtggct taccctggaa
     8581 ttcctaaact ggccccacag gttcataaca caacatctag agaactgaat gttagtaaaa
     8641 tcggctcctt tgctgaaccc tcttcagtgt cgttttcttc taaagaggcc ctctccttcc
     8701 cacacctcca tttgagaggg caaaggaatg atcgagacca acacacagat tctacccaat
     8761 cagcaaactc ctctccagat gaagatactg aagtcaaaac cttgaagcta tctggaatga
     8821 gcaacagatc atccattatc aacgaacata tgggatctag ttccagagat aggagacaga
     8881 aagggaaaaa atcctgtaaa gaaactttca aagaaaagca ttccagtaaa tcttttttgg
     8941 aacctggtca ggtgacaact ggtgaggaag gaaacttgaa gccagagttt atggatgagg
     9001 ttttgactcc tgagtatatg ggccaacgac catgtaacaa tgtttcttct gataagattg
     9061 gtgataaagg cctttctatg ccaggagtcc ccaaagctcc acccatgcaa gtagaaggat
     9121 ctgccaagga attacaggca ccacggaaac gcacagtcaa agtgacactg acacctctaa
     9181 aaatggaaaa tgagagtcaa tccaaaaatg ccctgaaaga aagtagtcct gcttcccctt
     9241 tgcaaataga gtcaacatct cccacagaac caatttcagc ctctgaaaat ccaggagatg
     9301 gtccagtggc ccaaccaagc cccaataata cctcatgcca ggattctcaa agtaacaact
     9361 atcagaatct tccagtacag gacagaaacc taatgcttcc agatggcccc aaacctcagg
     9421 aggatggctc ttttaaaagg aggtatcccc gtcgcagtgc ccgtgcacgt tctaacatgt
     9481 tttttgggct taccccactc tatggagtaa gatcctatgg tgaagaagac attccattct
     9541 acagcagctc aactgggaag aagcgaggca agagatcagc tgaaggacag gtggatgggg
     9601 ccgatgactt aagcacttca gatgaagacg acttatacta ttacaacttc actagaacag
     9661 tgatttcttc aggtggagag gaacgactgg catcccataa tttatttcgg gaggaggaac
     9721 agtgtgatct tccaaaaatc tcacagttgg atggtgttga tgatgggaca gagagtgata
     9781 ctagtgtcac agccacaaca aggaaaagca gccagattcc aaaaagaaat ggtaaagaaa
     9841 atggaacaga gaacttaaag attgatagac ctgaagatgc tggggagaaa gaacatgtca
     9901 ctaagagttc tgttggccac aaaaatgagc caaagatgga taactgccat tctgtaagca
     9961 gagttaaaac acagggacaa gattccttgg aagctcagct cagctcattg gagtcaagcc
    10021 gcagagtcca cacaagtacc ccctccgaca aaaatttact ggacacctat aatactgagc
    10081 tcctgaaatc agattcagac aataacaaca gtgatgactg tgggaatatc ctgccttcag
    10141 acattatgga ctttgtacta aagaatactc catccatgca ggctttgggt gagagcccag
    10201 agtcatcttc atcagaactc ctgaatcttg gtgaaggatt gggtcttgac agtaatcgtg
    10261 aaaaagacat gggtcttttt gaagtatttt ctcagcagct gcctacaaca gaacctgtgg
    10321 atagtagtgt ctcttcctct atctcagcag aggaacagtt tgagttgcct ctagagctac
    10381 catctgatct gtctgtcttg accacccgga gtcccactgt ccccagccag aatcccagta
    10441 gactagctgt tatctcagac tcaggggaga agagagtaac catcacagaa aaatctgtag
    10501 cctcctctga aagtgaccca gcactgctga gcccaggagt agatccaact cctgaaggcc
    10561 acatgactcc tgatcatttt atccaaggac acatggatgc agaccacatc tctagccctc
    10621 cttgtggttc agtagagcaa ggtcatggca acaatcagga tttaactagg aacagtagca
    10681 cccctggcct tcaggtacct gtttccccaa ctgttcccat ccagaaccag aagtatgtgc
    10741 ccaattctac tgatagtcct ggcccgtctc agatttccaa tgcagctgtc cagaccactc
    10801 caccccacct gaagccagcc actgagaaac tcatagttgt taaccagaac atgcagccac
    10861 tttatgttct ccaaactctt ccaaatggag tgacccaaaa aatccaattg acctcttctg
    10921 ttagttctac acccagtgtg atggagacaa atacttcagt attgggaccc atgggaggtg
    10981 gtctcaccct taccacagga ctaaatccaa gcttgccaac ttctcaatct ttgttccctt
    11041 ctgctagcaa aggattgcta cccatgtctc atcaccagca cttacattcc ttccctgcag
    11101 ctactcaaag tagtttccca ccaaacatca gcaatcctcc ttcaggcctg cttattgggg
    11161 ttcagcctcc tccggatccc caacttttgg tttcagaatc cagccagagg acagacctca
    11221 gtaccacagt agccactcca tcctctggac tcaagaaaag acccatatct cgtctacaga
    11281 cccgaaagaa taaaaaactt gctccctcta gtaccccttc aaacattgcc ccttctgatg
    11341 tggtttctaa tatgacattg attaacttca caccctccca gcttcctaat catccaagtc
    11401 tgttagattt ggggtcactt aatacttcat ctcaccgaac tgtccccaac atcataaaaa
    11461 gatctaaatc tagcatcatg tattttgaac cggcacccct gttaccacag agtgtgggag
    11521 gaactgctgc cacagcggca ggcacatcaa caataagcca ggatactagc cacctcacat
    11581 cagggtctgt gtctggcttg gcatccagtt cctctgtctt gaatgttgta tccatgcaaa
    11641 ctaccacaac ccctacaagt agtgcgtcag ttccaggaca cgtcacctta accaacccaa
    11701 ggttgcttgg taccccagat attggctcaa taagcaatct tttaatcaaa gctagccagc
    11761 agagcctggg gattcaggac cagcctgtgg ctttaccgcc aagttcagga atgtttccac
    11821 aactggggac atcacagacc ccctctactg ctgcaataac agcggcatct agcatctgtg
    11881 tgctcccctc cactcagact acgggcataa cagccgcttc accttctggg gaagcagacg
    11941 aacactatca gcttcagcat gtgaaccagc tccttgccag caaaactggg attcattctt
    12001 cccagcgtga tcttgattct gcttcagggc cccaggtatc caactttacc cagacggtag
    12061 acgctcctaa tagcatggga ctggagcaga acaaggcttt atcctcagct gtgcaagcca
    12121 gccccacctc tcctgggggt tctccatcct ctccatcttc tggacagcgg tcagcaagcc
    12181 cttcagtgcc gggtcccact aaacccaaac caaaaaccaa acggtttcag ctgcctctag
    12241 acaaagggaa tggcaagaag cacaaagttt cccatttgcg gaccagttct tctgaagcac
    12301 acattccaga ccaagaaacg acatccctga cctcaggcac agggactcca ggagcagagg
    12361 ctgagcagca ggatacagct agcgtggagc agtcctccca gaaggagtgt gggcaacctg
    12421 cagggcaagt cgctgttctt ccggaagttc aggtgaccca aaatccagca aatgaacaag
    12481 aaagtgcaga acctaaaaca gtggaagaag aggaaagtaa tttcagctcc ccactgatgc
    12541 tttggcttca gcaagaacaa aagcggaagg aaagcattac tgagaaaaaa cccaagaaag
    12601 gacttgtttt tgaaatttcc agtgatgatg gctttcagat ctgtgcagaa agtattgaag
    12661 atgcctggaa gtcattgaca gataaagtcc aggaagctcg atcaaatgcc cgcctaaagc
    12721 agctctcatt tgcaggtgtt aacggtttga ggatgctggg gattctccat gatgcagttg
    12781 tgttcctcat tgagcagctg tctggtgcca agcactgtcg aaattacaaa ttccgtttcc
    12841 acaagccaga ggaggccaat gaacccccct tgaaccctca cggctcagcc agggctgaag
    12901 tccacctcag gaagtcagca tttgacatgt ttaacttcct ggcttctaaa catcgtcagc
    12961 ctcctgaata caaccccaat gatgaagaag aggaggaggt acagctgaag tcagctcgga
    13021 gggcaactag catggatctg ccaatgccca tgcgcttccg gcacttaaaa aagacttcta
    13081 aggaggcagt tggtgtctac aggtctccca tccatggccg gggtcttttc tgtaagagaa
    13141 acattgatgc aggtgagatg gtgattgagt atgccggcaa cgtcatccgc tccatccaga
    13201 ctgacaagcg ggaaaagtat tacgacagca agggcattgg ttgctatatg ttccgaattg
    13261 atgactcaga ggtagtggat gccaccatgc atggaaatgc tgcacgcttc atcaatcact
    13321 cgtgtgagcc taactgctat tctcgggtca tcaatattga tgggcagaag cacattgtca
    13381 tctttgccat gcgtaagatc taccgaggag aggaactcac ttacgactat aagttcccca
    13441 ttgaggatgc cagcaacaag ctgccctgca actgtggcgc caagaaatgc cggaagttcc
    13501 taaacgttta aacgaattcg ggctcggtac ccggggatcc tctagagtcg acctgcaggc
    13561 atgcaagctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
    13621 gctgagcaat aactagcata accccttggg gcggccgctt cgagcagaca tgataagata
    13681 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
    13741 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
    13801 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag
    13861 caagtaaaac ctctacaaat gtggtaaaat cgaattttaa caaaatatta acgcttacaa
    13921 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg
    13981 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
    14041 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
    14101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
    14161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    14221 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    14281 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    14341 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    14401 cttaattaac tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
    14461 aggtgaggaa ctaaacccag gaggcagatc atgattgaac aagatggatt gcacgcaggt
    14521 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    14581 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    14641 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
    14701 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    14761 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    14821 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    14881 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    14941 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    15001 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    15061 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    15121 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    15181 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    15241 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
    15301 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct
    15361 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcctc
    15421 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
    15481 caccgtttct gcggactggc tttctacccg gtatcagctc actcaaaggc ggtaatacgg
    15541 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    15601 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    15661 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    15721 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    15781 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    15841 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    15901 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    15961 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    16021 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    16081 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    16141 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    16201 acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct
    16261 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    16321 acctagatcc ttttatagtc cggaaataca ggaacgcacg ctggatggcc cttcgctggg
    16381 atggtgaaac catgaaaaat ggcagcttca gtggattaag tgggggtaat gtggcctgta
    16441 ccctctggtt gcataggtat tcatacggtt aaaatttatc aggcgcgatt gcggcagttt
    16501 ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttt
    16561 agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat
    16621 cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg agatct
//
