LOCUS       Exported                5707 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN21A(pF-47nA)-WDR5      5707 bp    DNA   circular     
                  10/21/2016

            DEFINITION  Current Name: pFN21A (pF-47nA) - WDR5 Old/Alternate 
            Name(s):BIG-3 (insert) SWD3 (insert) WD repeat domain 5 (insert) 
            WDR5 (insert) pF-47nA (backbone)
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     misc_feature    992..997
                     /label=6base
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1067..3013
                     /label=Complete ORF
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:43:25 AM"
     misc_feature    1067..1957
                     /label=HT7
     misc_feature    complement(1859..1864)
                     /label=6base
     misc_feature    1958..1996
                     /label=HT7 Linker N terminal
     misc_feature    1970..1990
                     /label=TEV protease recognition sequence
     misc_feature    1997..2004
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2001..3011
                     /label=WDR5 insert
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:43:25 AM"
     misc_feature    2353..2358
                     /label=EcoICR I
     misc_feature    3008..3015
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3012..3145
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    3167..3388
                     /label=Sv40 late polyadenylation signal
     CDS             3649..4509
                     /label=beta-lactamase
     rep_origin      4664..4700
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    5371..5656
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgccatggc gacggaggag aagaagcccg agaccgaggc
     2041 cgccagagca cagccaaccc cttcgtcatc cgccactcag agcaagccta cacctgtgaa
     2101 gccaaactat gctctaaagt tcacccttgc tggccacacc aaagcagtgt cctccgtgaa
     2161 attcagcccg aatggagagt ggctggcaag ttcatctgct gataaactta ttaaaatttg
     2221 gggcgcgtat gatgggaaat ttgagaaaac catatctggt cacaagctgg gaatatccga
     2281 tgtagcctgg tcgtcagatt ctaaccttct tgtttctgcc tcagatgaca aaaccttgaa
     2341 gatatgggac gtgagctcgg gcaagtgtct gaaaaccctg aagggacaca gtaattatgt
     2401 cttttgctgc aacttcaatc cccagtccaa ccttattgtc tcaggatcct ttgacgaaag
     2461 cgtgaggata tgggatgtga aaacagggaa gtgcctcaag actttgccag ctcactcgga
     2521 tccagtctcg gccgttcatt ttaatcgtga tggatccttg atagtttcaa gtagctatga
     2581 tggtctctgt cgcatctggg acaccgcctc aggccagtgc ctgaagacgc tcatcgatga
     2641 cgacaacccc cccgtgtctt ttgtgaagtt ctccccgaac ggcaaataca tcctggccgc
     2701 cacgctggac aacactctga agctctggga ctacagcaag gggaagtgcc tgaagacgta
     2761 cactggccac aagaatgaga aatactgcat atttgccaat ttctctgtta ctggtgggaa
     2821 gtggattgtg tctggctcag aggataacct tgtttacatc tggaaccttc agacgaaaga
     2881 gattgtacag aaactacaag gccacacaga tgtcgtgatc tcaacagctt gtcacccaac
     2941 agaaaacatc atcgcctctg ctgcgctaga aaatgacaaa acaattaaac tgtggaagag
     3001 tgactgcgtt taaacgaatt cgggctcggt acccggggat cctctagagt cgacctgcag
     3061 gcatgcaagc tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     3121 ccgctgagca ataactagca taaccccttg gggcggccgc ttcgagcaga catgataaga
     3181 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3241 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3301 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta
     3361 agcaagtaaa acctctacaa atgtggtaaa atcgaattct aatggatcct ctttgcgctt
     3421 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     3481 tgcggactgg ctttctacgt aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3541 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3601 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3661 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3721 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3781 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3841 ccaatgatga gcactttcaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3901 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3961 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4021 ataaccatga gtgataacac tgcggccaac ttacttctga caactatcgg aggaccgaag
     4081 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4141 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4201 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4261 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4321 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4381 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4441 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4501 cattggtaat tcgaaatgac cgaccaagcg acgcccaacc ggtatcagct cactcaaagg
     4561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5101 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5221 agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg
     5281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5341 aaaggatctt cacctagatc cttttatagt ccggaaatac aggaacgcac gctggatggc
     5401 ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa
     5461 tgtggcctgt accctctggt tgcataggta ttcatacggt taaaatttat caggcgcgat
     5521 tgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgct
     5581 gaacgcgttt tagcggtgcg tacaattaag ggattatggt aaatccactt actgtctgcc
     5641 ctcgtagcca tcgagataaa ccgcagtact ccggccacga tgcgtccggc gtagaggatc
     5701 gagatct
//
