LOCUS       Exported                9847 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    301..4578
                     /label=BCL9
                     /note="BCL9"
     misc_feature    complement(4580..4587)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(4588..4599)
                     /label=linker
                     /note="linker"
     misc_feature    complement(4600..5112)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    5126..5152
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(5153..5169)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(5172..5217)
                     /label=Minimal CMV Promoter 2
     misc_feature    5195..5217
                     /label=core
                     /note="core"
     promoter        5227..5781
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        5799..5865
                     /label=EM7 bacterial promoter
     CDS             5879..6538
                     /label=Chloramphenicol resistance
     rep_origin      6707..6743
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(7425..7472)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(7496..8290)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(8304..8598)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(8669..9847)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 aaacatcatg tttcctgggt tgccaggtcc ttggctaaat ccacccatgc ccacgtcagc
      361 agccatgccc cggggcctca tctgtggggg gatcatgatg ttctgttggg gtcccatcat
      421 gccctgcatg gacatcatca tgcctggaga gcccacaggc ccagggtggg tgtagagccc
      481 ggcaggcccc cgatccttgc caggaatcat gcccacggca gcagctggat tgctcatcag
      541 ggtgggctgg ccgggcattg tagattgtgc tggtgacatc atccgatggt gaggtcccat
      601 catgccttgt tggagaaagg ctggtggtct catggggttg tggcctggca tggatggagc
      661 tgtaccaaga gggatgtccg gagttcccac tggccctgga cctcccatgc caggtaatgc
      721 tagtcccatt ctgggggctt gttcgcccat catcccctgc atgtgtgaaa acccaggtcc
      781 aggaccctgg ggctgtttac ggcctggaac ttcccctcga gggaaatatt gcagcgtctg
      841 gctgggcttc tcagatggaa taatgcggga tagatcaaac tcaggtattc cggtggctcc
      901 aggtcggatg acctcctgca gatctgggtc tgtaaacacc gaaggcatgc tgttccccag
      961 gacagtgaag gagtcaggac ccccggggcc tccaggcttg caaagtgctg catctgctga
     1021 actttggggc aggttgctgg ggcggccaag ggggccttct cctgggaaac ccatccctcc
     1081 tgggaagctg ccctgccccc cactggggcc attgtgaggg aatggaacct gctgtggggg
     1141 agactgtact ggagggaagc cctgggggaa gcccatccgt ccttgaggta ccatcggtgg
     1201 ctcctgggac ccatgcccca tgataggatt gtgtgacatc aagccaggcc ccattgggac
     1261 cccatgagga ggtatgttgg gtcccacggc atttggagag ggcatctgat tggagtgaga
     1321 aagtggctgg gtcattccca ttgggctgag ggttggcatc ggaaccacgg ggtttggacc
     1381 tgaaattcga ggattctgtg tattaatgcc cattcctggc atattattca ttgatggcaa
     1441 gttgggagaa cgagctggag gggagtcgtc atctgagctg gccacagtct tgatagcatc
     1501 atggtataac ggggtggaac tgggcattgc aaacttggac attcgagaca tcataataga
     1561 gagtgggttc tgggaaaggg ttggctctgg aggcatggta taaggtgtac tagagggaag
     1621 acttccaggg atattcacag aggcaggctg gctggctgta ggaggtgggg ggccacctga
     1681 ctctacattt cccagcatgg ctggggaggc catggtgagg ggtgctttat ggtttggagg
     1741 gatcccagga ctctgaaggg gaggttttgg agaagaggtc catccaggtg acggggcagg
     1801 aagtgatgga gacttgaggt ggacaggtga agcagcagca gaccccaaaa caggggggga
     1861 cttaatggaa gcagcagcag ctgggcccgc cagcatgcct gccagctgcg atggagtctg
     1921 gggggacttg aggttccccg agggcgagcc cagcattggt gactggactt ggtgcatcgt
     1981 gggagacttc agagggttaa tgcctgggga atgcacctgg ctgcctgcca cagatatatc
     2041 caagggcttc cgccccaggc cgcgctgaac tggaggagct gtgttgaggc tggtggggtt
     2101 actggaaggg ttgagaggta gtggtggcat atgactgagc cggctgttag tcctctggtc
     2161 gggcccaatt ggttctctga gattccgcaa gccactgttg ctgcctggac cctgagacat
     2221 gggaaggaat ggtctggggc ccatgccata ctcctgctgg gggtgctcac caaatggcag
     2281 tggcaccatc ttctgctgag caggcagcat gtctgagcca cctgggcgta atttcagcat
     2341 ctcctcaggg cccgccccag cctctctcat cttctgaggt atcatctgag agttggatcc
     2401 catgttgaca tttagattga catctccctt catcccacta ggaaccatcc caaactcaag
     2461 ttcccgacca gggcccatct gtgggggaat tccttttgga aagtcacccc tagaaggact
     2521 cagagggccc tcaactggta ttcgagggaa aatggggtta ttcccaggct ccatgtggcg
     2581 ctgggagcct ggaatcatcc tgttcatctc catgctgggc ctgatgcctt ccatggccat
     2641 ccctgggggg agacccagct gtttctctgc cagctgctgc tgaaacatct cttcagacaa
     2701 tccttggggg tttgggaagc gttcccctcg gccaggaccg ctgaaaatgc cctggccagg
     2761 aggaaaattt cgaccatctg ggatttttgg cacatcatct ggccaactga ctccagaaag
     2821 acctggtcta gatgcagggt tggggacatt cggcccttcc atttcagagt ttatcatgcc
     2881 tgcaaatcca gggaggcgca tctggctccc tggcatgttg gggtggggag ccatgcccct
     2941 cgggggcaga gaatgtggca tgttgatacc atcagaaaat ggctctgtac ccccaggtgc
     3001 ccagccttca ctaggggtca tctggtatgg aggggggggt cctcggacca ctccccgagg
     3061 cccgtgctga tggaccatca tgtcctggag ggaacactgc tggacaacca cttgttcctg
     3121 cttcctcctc ttctcttcat aaaactcctg ctgcagtttc agccacgcta tctgctcggg
     3181 agtcatatgg tcaaggtggt cgggtcctat ggtcccagac tgggagttca tagaaggtgg
     3241 aaccatttca tctggagaaa agggtacatc tctatgtcct tgagggccaa atggagctcc
     3301 cacgtctgtc cggggcccag gtcccttacc taggctttgg gattgggcca tcatggcctg
     3361 tattggccct tctggttttt tctgaggccc atctaatacc ccaggattct gctgcggtcc
     3421 cccactttgt gctcctgtga attctttctc atcaggaaaa agcatgcgct ggatatctct
     3481 gagagtttgt aaggagcgct cccggtgctc cagctgctcc tgagataggc catcgggatt
     3541 ctctcccagt gtggggggct cgccactgga cactggtgga ggcggagggg ctttgggatc
     3601 tgctgaagag ctattgctcc cctgggagac aggggtcact gccctattgt tgggagttga
     3661 gttgggccca gtaccatctg ggggcagtgg agtggagctg gcaggacttc ctacagaagg
     3721 aatcagtttg ttttctaccc caggactctc ccggtccagg ggacgtgggg gtgcggcagg
     3781 cttgggtgct ggtgccggaa tgggtggagt tggctgcagt ctggtattct gggaagaatt
     3841 ctggtcctgg ttggccggag ctgggggctg ttgtgggaga ggtttcggat cattccgaag
     3901 ggcagatatc tgtgtgttca gaggcgctgt gcttctctct gtcttgttgt tagaaatgtt
     3961 ctggatgtgg aaagagacga tagtttcaac ctggcccttc aaaacagctt ctgcagcttt
     4021 attggccatc tcagtagaaa acacgtacac cactttggct ggagtcttct gagcaggtgt
     4081 gggctctgtg gcagtagttt ggccatggga gggggtagaa gacctggggg ctgtagcatt
     4141 tgatggggtc atcgagtgtg gtgtgtgctg ggaatcctgg gactttatgt ggtcagcaga
     4201 attacattct ttaatgtcag agtcatcgtt tggagtccca ggatctctct gatcaaagga
     4261 gtcggcggaa atacttcgct cccttttccc cttgcccttg gcaccatttc cagccccatt
     4321 cttcagcccc atgctcccac ctgggccagg gagtgcttta ggggtatggc ccccactctt
     4381 ggagtcacag ggggatggct gggattggct ggctgagccc ccctgtttac cctgattgga
     4441 gaatttggaa tccagctggg ggtttccaga tggggacatc actgtagggg gacggaccat
     4501 cacctcctgc tttgacttag ggctactctg tgtgtttcct gatggagagc tcctcacttt
     4561 agggttactg gaatgcatgg cgatcgcgcc gctcgagccc gccagaatgc gttcgcacag
     4621 ccgccagccg gtcactccgt tgatggttac tcggaacagc agggagccgt cggggttgat
     4681 caggcgctcg tcgataattt tgttgccgtt ccacagggtc cctgttacag tgatcttttt
     4741 gccgtcgaac acggcgatgc cttcatacgg ccgtccgaaa tagtcgatca tgttcggcgt
     4801 aaccccgtcg attaccagtg tgccatagtg caggatcacc ttaaagtgat gatcatccac
     4861 agggtacacc accttaaaaa ttttttcgat ctggcccatt tggtcgccgc tcagaccttc
     4921 atacgggatg atgacatgga tgtcgatctt cagcccattt tcaccgctca ggacaatcct
     4981 ttggatcgga gttacggaca ccccgagatt ctgaaacaaa ctggacacac ctccctgttc
     5041 aaggacttgg tccaggttgt agccggctgt ctgtcgccag tccccaacga aatcttcgag
     5101 tgtgaagacc atggtgagcg ctagctgcaa aaagaacaag tagcttgtat tctatagtgt
     5161 cacctaaatg ggcgatctga cggttcacta aaccagctct gcttatatag gcctccctgg
     5221 cgcgccacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5281 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5341 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5401 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5461 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5521 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     5581 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     5641 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     5701 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     5761 aggcgtgtac ggtgggaggt caaatcgaat tctaatactg ttgacaatta atcatcggca
     5821 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
     5881 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     5941 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     6001 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     6061 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
     6121 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     6181 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     6241 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     6301 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     6361 caatatggac aacttcttcg cccccgtttt cactatgggc aaatattata cgcaaggcga
     6421 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt
     6481 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaagc
     6541 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa
     6601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7261 gcaagcagca gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta
     7321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7381 caaaaaggat cttcacctag atccttttat agtccggaat cgtcacacaa aaaaccaaca
     7441 cacagatgta atgaaaataa agatatttta ttgcggccat cgtgatggct agagttcaga
     7501 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     7561 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     7621 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     7681 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccgtgg gtcacgacga
     7741 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     7801 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     7861 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     7921 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     7981 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     8041 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     8101 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     8161 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     8221 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     8281 gttcaatcat ggtggcatcg attcccggtc aacatccagt acacctccgg caccaccggc
     8341 ttccccaagg gcgccacgct ctcccaccac aacatcctca acaacggcta ctgggtgggc
     8401 cgcacggtcg gctacacgga gcaggaccgg gtgtgtctgc cggtgccctt ctaccactgc
     8461 ttcggcatgg tcatgggcaa cttgggcgcc acctcccacg ccgcctgcat cgtcatcccg
     8521 gccccgtcct tcgagccggc ggcgacgctg gaggcggtgc agcgggagag gtgcacgtcc
     8581 ctgtacggcg tccccacgaa gccgaattcc accacactgg actagatgca tgctcgacag
     8641 tgtgatggat atctgcagaa ttcggctttc acgacacctg aaatggaaga aaaaaacttt
     8701 gaaccactgt ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg gcaaattcca
     8761 aggagaatta catcaagtgc caagctggcc taacttcagt ctccacccac tcagtgtggg
     8821 gaaactccat cgcataaaac ccctcccccc aacctaaaga cgacgtactc caaaagctcg
     8881 agaactaatc gaggtgcctg gacggcgccc ggtactccgt ggagtcacat gaagcgacgg
     8941 ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc acccgcccgc tctcccgagc
     9001 gccgcgtcct ccattttgag ctgcctgcag cagggccggg aagcggccat ctttccgctc
     9061 acgcaactgg tgccgaccgg gccagccttg ccgcccaggg cggggcgata cacggcggcg
     9121 cgaggccagg caccagagca ggccggccag cttgagacta cccccgtccg attctcggtg
     9181 gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg gccccgtcgc
     9241 cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc ttggcccgca tttacaagac
     9301 tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa attttaaatg gctagagact
     9361 tatcgaaagc agcgagacag gcgcgaaggt gccaccagat tcgcacgcgg cggccccagc
     9421 gcccaggcca ggcctcaact caagcacgag gcgaaggggc tccttaagcg caaggcctcg
     9481 aactctccca cccacttcca acccgaagct cgggatcaag aatcacgtac tgcagccagg
     9541 tggaagtaat tcaaggcacg caagggccat aacccgtaaa gaggccaggc ccgcgggaac
     9601 cacacacggc acttacctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg
     9661 cgactactgc acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca
     9721 cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga
     9781 cccctccccc caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac
     9841 cggagcc
//
