LOCUS       Exported                6015 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3-SKP2      6015 bp    DNA   circular            
            10/2/2013

            DEFINITION  Current Name: pF-Nc5K3 - SKP2 Old/Alternate Name(s):FBL1
            (insert) FBXL1 (insert) FLB1 (insert) KIBB4066 (insert) MGC1366 
            (insert) SKP2 (insert) S-phase kinase-associated protein 2 (p45) 
            (insert)
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..2340
                     /label=SKP2
                     /note="Created by Kazusa. Available from Kazusa. Data 
                     recieved from Kazusa 11/2008. Entered by Ethan 12/2008.  
                     End definitions corrected 5/2010. Name changed from 
                     KIBB4066 to SKP2 by Ethan 5/17/2010. (note added in 
                     estrauss by 5/17/2010 3:03:39 PM"
     misc_feature    2343..2357
                     /label=linker
     CDS             2358..2867
                     /label=C terminal NanoLuc
     polyA_signal    3004..3225
                     /label=Sv40 late polyadenylation signal
     promoter        3324..3742
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3750..3816
                     /label=EM7 bacterial promoter
     CDS             3830..4624
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4688..4736
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      4972..5008
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcac aggaagcacc
     1081 tccaggagat tccagacctg agtagcaacg ttgccaccag cttcacgtgg ggatgggatt
     1141 ccagcaagac ttctgaactg ctgtcaggca tgggggtctc cgccctggag aaagaggagc
     1201 ccgacagtga gaacatcccc caggaactgc tctcaaacct gggccacccg gagagccccc
     1261 cacggaaacg gctgaagagc aaagggagtg acaaagactt tgtgattgtc cgcaggccta
     1321 agctaaatcg agagaacttt ccaggtgttt catgggactc ccttccggat gagctgctct
     1381 tgggaatctt ttcctgtctg tgcctccctg agctgctaaa ggtctctggt gtttgtaaga
     1441 ggtggtatcg cctagcgtct gatgagtctc tatggcagac cttagacctc acaggtaaaa
     1501 atctgcaccc ggatgtgact ggtcggttgc tgtctcaagg ggtgattgcc ttccgctgcc
     1561 cacgatcatt tatggaccaa ccattggctg aacatttcag cccttttcgt gtacagcaca
     1621 tggacctatc gaactcagtt atagaagtgt ccaccctcca cggcatactg tctcagtgtt
     1681 ccaagttgca gaatctaagc ctggaaggcc tgcggctttc ggatcccatt gtcaatactc
     1741 tcgcaaaaaa ctcaaattta gtgcgactta acctttctgg gtgttctgga ttctctgaat
     1801 ttgccctgca gactttgcta agcagctgtt ccagactgga tgagctgaac ctctcctggt
     1861 gttttgattt cactgaaaag catgtacagg tggctgttgc gcatgtgtca gagaccatca
     1921 cccagctgaa tcttagcggc tacagaaaga atctccagaa atcagatctc tctactttag
     1981 ttagaagatg ccccaatctt gtccatctag acttaagtga tagtgtcatg ctaaagaatg
     2041 actgctttca ggaatttttc cagctcaact acctccaaca cctatcactc agtcggtgct
     2101 atgatataat acctgaaact ttacttgaac ttggagaaat tcccacacta aaaacactac
     2161 aagtttttgg aatcgtgcca gatggtaccc ttcaactgtt aaaggaagcc cttcctcatc
     2221 tacagattaa ttgctcccat ttcaccacca ttgccaggcc aactattggc aacaaaaaga
     2281 accaggagat atggggcatc aaatgccgac tgacactgca aaagcccagt tgtctagttt
     2341 ctctcggctc gagcggcgtc ttcacactcg aagatttcgt tggggactgg cgacagacag
     2401 ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc
     2461 tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga
     2521 tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg
     2581 aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact
     2641 atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt
     2701 atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg
     2761 gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa
     2821 ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa ggccgcgact
     2881 ctagagtcga cctgcaggca tgcaagctga tccggctgct aacaaagccc gaaaggaagc
     2941 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cggccgcttc
     3001 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3061 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3121 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     3181 tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc gaattttaac
     3241 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3301 tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     3361 aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     3421 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3481 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3541 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3601 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3661 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3721 cctaggcttt tgcaaaaagc ttaattaact gttgacaatt aatcatcggc atagtatatc
     3781 ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca tgattgaaca
     3841 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3901 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3961 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     4021 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4081 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4141 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4201 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4261 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4321 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     4381 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4441 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4501 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4561 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4621 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     4681 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     4741 gatagcgata aggatcctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     4801 gacattcatc cggggtcagc accgtttctg cggactggct ttctacccgg tatcagctca
     4861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5161 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt
     5581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5641 attatcaaaa aggatcttca cctagatcct tttatagtcc ggaaatacag gaacgcacgc
     5701 tggatggccc ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag tggattaagt
     5761 gggggtaatg tggcctgtac cctctggttg cataggtatt catacggtta aaatttatca
     5821 ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg
     5881 atcgcgctga acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac
     5941 tgtctgccct cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtccggcgt
     6001 agaggatcga gatct
//
