LOCUS       Exported                7036 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN21A(pF-47nA)-NR3C1      7036 bp    DNA   circular    
                   9/6/2012

            DEFINITION  Current Name: pFN21A (pF-47nA) - NR3C1 Old/Alternate 
            Name(s):GCCR (insert) GCR (insert) GR (insert) GRL (insert) NR3C1 
            (insert) nuclear receptor subfamily 3, group C, member 1 
            (glucocorticoid receptor) (insert) pF-47nA (backbone)
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     CDS             1067..4342
                     /label=Complete ORF (with fusions) of NR3C1
                     /note="Data entered semi automatically by Ethan on 
                     7/18/2011"
     misc_feature    1067..1957
                     /label=HT7
                     /note="Amino Acid sequence is identical to other HT7 
                     versions, but different DNA sequence."
     misc_feature    1958..1996
                     /label=HT7 Linker N terminal
     misc_feature    1970..1990
                     /label=TEV protease recognition sequence
     misc_feature    1997..2004
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2001..4340
                     /label=NR3C1 insert
                     /note="Data entered semi automatically by Ethan on 
                     7/18/2011"
     misc_feature    complement(2888..2894)
                     /label=Pme I EcoICRI Chimeric Site
                     /note=" Feature was found in the reverse orientation."
     misc_feature    4337..4344
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    4341..4474
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    4496..4717
                     /label=Sv40 late polyadenylation signal
     CDS             4978..5838
                     /label=Beta-Lactamase coding region
                     /note="Removed RE site from standard beta-lactamase gene"
     rep_origin      5993..6029
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6700..6985
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gccaccatgg cagaaatcgg
     1081 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     1141 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     1201 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     1261 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     1321 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     1381 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     1441 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     1501 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     1561 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     1621 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     1681 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     1741 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     1801 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     1861 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     1921 gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg aggatctgta
     1981 ctttcagagc gataacgcga tcgccatgga ctccaaagaa tcattaactc ctggtagaga
     2041 agaaaacccc agcagtgtgc ttgctcagga gaggggagat gtgatggact tctataaaac
     2101 cctaagagga ggagctactg tgaaggtttc tgcgtcttca ccctcactgg ctgtcgcttc
     2161 tcaatcagac tccaagcagc gaagactttt ggttgatttt ccaaaaggct cagtaagcaa
     2221 tgcgcagcag ccagatctgt ccaaagcagt ttcactctca atgggactgt atatgggaga
     2281 gacagaaaca aaagtgatgg gaaatgacct gggattccca cagcagggcc aaatcagcct
     2341 ttcctcgggg gaaacagact taaagctttt ggaagaaagc attgcaaacc tcaataggtc
     2401 gaccagtgtt ccagagaacc ccaagagttc agcatccact gctgtgtctg ctgcccccac
     2461 agagaaggag tttccaaaaa ctcactctga tgtatcttca gaacagcaac atttgaaggg
     2521 ccagactggc accaacggtg gcaatgtgaa attgtatacc acagaccaaa gcacctttga
     2581 cattttgcag gatttggagt tttcttctgg gtccccaggt aaagagacga atgagagtcc
     2641 ttggagatca gacctgttga tagatgaaaa ctgtttgctt tctcctctgg cgggagaaga
     2701 cgattcattc cttttggaag gaaactcgaa tgaggactgc aagcctctca ttttaccgga
     2761 cactaaaccc aaaattaagg ataatggaga tctggttttg tcaagcccca gtaatgtaac
     2821 actgccccaa gtgaaaacag aaaaagaaga tttcatcgaa ctctgcaccc ctggggtaat
     2881 taagcaagag aaactgggca cagtttactg tcaggcaagc tttcctggag caaatataat
     2941 tggtaataaa atgtctgcca tttctgttca tggtgtgagt acctctggag gacagatgta
     3001 ccactatgac atgaatacag catccctttc tcaacagcag gatcagaagc ctatttttaa
     3061 tgtcattcca ccaattcccg ttggttccga aaattggaat aggtgccaag gatctggaga
     3121 tgacaacttg acttctctgg ggactctgaa cttccctggt cgaacagttt tttctaatgg
     3181 ctattcaagc cccagcatga gaccagatgt aagctctcct ccatccagct cctcaacagc
     3241 aacaacagga ccacctccca aactctgcct ggtgtgctct gatgaagctt caggatgtca
     3301 ttatggagtc ttaacttgtg gaagctgtaa agttttcttc aaaagagcag tggaaggaca
     3361 gcacaattac ctatgtgctg gaaggaatga ttgcatcatc gataaaattc gaagaaaaaa
     3421 ctgcccagca tgccgctatc gaaaatgtct tcaggctgga atgaacctgg aagctcgaaa
     3481 aacaaagaaa aaaataaaag gaattcagca ggccactaca ggagtctcac aagaaacctc
     3541 tgaaaatcct ggtaacaaaa caatagttcc tgcaacgtta ccacaactca cccctaccct
     3601 ggtgtcactg ttggaggtta ttgaacctga agtgttatat gcaggatatg atagctctgt
     3661 tccagactca acttggagga tcatgactac gctcaacatg ttaggagggc ggcaagtgat
     3721 tgcagcagtg aaatgggcaa aggcaatacc aggtttcagg aacttacacc tggatgacca
     3781 aatgacccta ctgcagtact cctggatgtt tcttatggca tttgctctgg ggtggagatc
     3841 atatagacaa tcaagtgcaa acctgctgtg ttttgctcct gatctgatta ttaatgagca
     3901 gagaatgact ctaccctgca tgtacgacca atgtaaacac atgctgtatg tttcctctga
     3961 gttacacagg cttcaggtat cttatgaaga gtatctctgt atgaaaacct tactgcttct
     4021 ctcttcagtt cctaaggacg gtctgaagag ccaagagcta tttgatgaaa ttagaatgac
     4081 ctacatcaaa gagctaggaa aagccattgt caagagggaa ggaaactcca gccagaactg
     4141 gcagcggttt tatcaactga caaaactctt ggattctatg catgaagtgg ttgaaaatct
     4201 ccttaactat tgcttccaaa catttttgga taagaccatg agtattgaat tccccgagat
     4261 gttagctgaa atcatcacca atcagatacc aaaatattca aatggaaata tcaaaaaact
     4321 tctgtttcat caaaaggttt aaacgaattc gggctcggta cccggggatc ctctagagtc
     4381 gacctgcagg catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     4441 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac
     4501 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4561 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4621 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     4681 gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgaattcta atggatcctc
     4741 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     4801 caccgtttct gcggactggc tttctacgta atggtttctt agacgtcagg tggcactttt
     4861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5161 gaacgttttc caatgatgag cactttcaaa gttctgctat gtggcgcggt attatcccgt
     5221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aactatcgga
     5401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5821 ctgattaagc attggtaatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc
     5881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct
     6601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6661 gattatcaaa aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg
     6721 ctggatggcc cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag
     6781 tgggggtaat gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc
     6841 aggcgcgatt gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt
     6901 gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta
     6961 ctgtctgccc tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtccggcg
     7021 tagaggatcg agatct
//
