LOCUS       Exported                7107 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       UnnamedSequence      5152 bp    DNA   circular          
             9/13/2011

            DEFINITION  Current Name: Unnamed Sequence
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     misc_feature    1056..1060
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1061..3399
                     /label=UnnamedSequence
     misc_feature    1061..3399
                     /label=Untitled
     misc_feature    1061..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     gene            1065..3398
                     /label=Glucocorticoid Receptor [GR]
                     /note="from Kazusa pFN21AB9466"
     misc_feature    complement(1947..1953)
                     /label=Pme I EcoICRI Chimeric Site
                     /note=" Feature was found in the reverse orientation."
     misc_feature    3396..3399
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_structure  3399..3449
                     /label=linker
                     /note="linker"
     misc_feature    3400..3402
                     /label=EcoICR I
     gene            3450..3959
                     /label=NL02A
                     /note="NL02A"
     polyA_signal    4096..4317
                     /label=Sv40 late polyadenylation signal
     promoter        4416..4834
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(4489..4725)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      4732..4797
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        4842..4908
                     /label=EM7 bacterial promoter
     CDS             4922..5716
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5780..5828
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      6064..6100
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6771..7056
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggac tccaaagaat
     1081 cattaactcc tggtagagaa gaaaacccca gcagtgtgct tgctcaggag aggggagatg
     1141 tgatggactt ctataaaacc ctaagaggag gagctactgt gaaggtttct gcgtcttcac
     1201 cctcactggc tgtcgcttct caatcagact ccaagcagcg aagacttttg gttgattttc
     1261 caaaaggctc agtaagcaat gcgcagcagc cagatctgtc caaagcagtt tcactctcaa
     1321 tgggactgta tatgggagag acagaaacaa aagtgatggg aaatgacctg ggattcccac
     1381 agcagggcca aatcagcctt tcctcggggg aaacagactt aaagcttttg gaagaaagca
     1441 ttgcaaacct caataggtcg accagtgttc cagagaaccc caagagttca gcatccactg
     1501 ctgtgtctgc tgcccccaca gagaaggagt ttccaaaaac tcactctgat gtatcttcag
     1561 aacagcaaca tttgaagggc cagactggca ccaacggtgg caatgtgaaa ttgtatacca
     1621 cagaccaaag cacctttgac attttgcagg atttggagtt ttcttctggg tccccaggta
     1681 aagagacgaa tgagagtcct tggagatcag acctgttgat agatgaaaac tgtttgcttt
     1741 ctcctctggc gggagaagac gattcattcc ttttggaagg aaactcgaat gaggactgca
     1801 agcctctcat tttaccggac actaaaccca aaattaagga taatggagat ctggttttgt
     1861 caagccccag taatgtaaca ctgccccaag tgaaaacaga aaaagaagat ttcatcgaac
     1921 tctgcacccc tggggtaatt aagcaagaga aactgggcac agtttactgt caggcaagct
     1981 ttcctggagc aaatataatt ggtaataaaa tgtctgccat ttctgttcat ggtgtgagta
     2041 cctctggagg acagatgtac cactatgaca tgaatacagc atccctttct caacagcagg
     2101 atcagaagcc tatttttaat gtcattccac caattcccgt tggttccgaa aattggaata
     2161 ggtgccaagg atctggagat gacaacttga cttctctggg gactctgaac ttccctggtc
     2221 gaacagtttt ttctaatggc tattcaagcc ccagcatgag accagatgta agctctcctc
     2281 catccagctc ctcaacagca acaacaggac cacctcccaa actctgcctg gtgtgctctg
     2341 atgaagcttc aggatgtcat tatggagtct taacttgtgg aagctgtaaa gttttcttca
     2401 aaagagcagt ggaaggacag cacaattacc tatgtgctgg aaggaatgat tgcatcatcg
     2461 ataaaattcg aagaaaaaac tgcccagcat gccgctatcg aaaatgtctt caggctggaa
     2521 tgaacctgga agctcgaaaa acaaagaaaa aaataaaagg aattcagcag gccactacag
     2581 gagtctcaca agaaacctct gaaaatcctg gtaacaaaac aatagttcct gcaacgttac
     2641 cacaactcac ccctaccctg gtgtcactgt tggaggttat tgaacctgaa gtgttatatg
     2701 caggatatga tagctctgtt ccagactcaa cttggaggat catgactacg ctcaacatgt
     2761 taggagggcg gcaagtgatt gcagcagtga aatgggcaaa ggcaatacca ggtttcagga
     2821 acttacacct ggatgaccaa atgaccctac tgcagtactc ctggatgttt cttatggcat
     2881 ttgctctggg gtggagatca tatagacaat caagtgcaaa cctgctgtgt tttgctcctg
     2941 atctgattat taatgagcag agaatgactc taccctgcat gtacgaccaa tgtaaacaca
     3001 tgctgtatgt ttcctctgag ttacacaggc ttcaggtatc ttatgaagag tatctctgta
     3061 tgaaaacctt actgcttctc tcttcagttc ctaaggacgg tctgaagagc caagagctat
     3121 ttgatgaaat tagaatgacc tacatcaaag agctaggaaa agccattgtc aagagggaag
     3181 gaaactccag ccagaactgg cagcggtttt atcaactgac aaaactcttg gattctatgc
     3241 atgaagtggt tgaaaatctc cttaactatt gcttccaaac atttttggat aagaccatga
     3301 gtattgaatt ccccgagatg ttagctgaaa tcatcaccaa tcagatacca aaatattcaa
     3361 atggaaatat caaaaaactt ctgtttcatc aaaaggtttc tctcggtggt tcaggtggtg
     3421 gcgggagcgg tggagggagc agcggtggag tcttcacact cgaagatttc gttggggact
     3481 ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt
     3541 tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa
     3601 atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa
     3661 tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg
     3721 tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt
     3781 tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga
     3841 ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc
     3901 tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcgt
     3961 aaggccgcga ctctagagtc gacctgcagg catgcaagct gatccggctg ctaacaaagc
     4021 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     4081 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     4141 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4201 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     4261 ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa
     4321 tcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     4381 atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac
     4441 ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt
     4501 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4561 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4621 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4681 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4741 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4801 cttttttgga ggcctaggct tttgcaaaaa gcttaattaa ctgttgacaa ttaatcatcg
     4861 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccca ggaggcagat
     4921 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4981 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5041 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5101 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5161 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5221 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5281 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5341 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5401 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5461 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5521 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5581 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5641 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5701 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5761 ctgccatcac gatggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     5821 tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt gcgttttccc ttgtccagat
     5881 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctaccc
     5941 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6001 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6061 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6121 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6181 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6241 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6301 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6361 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6421 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6481 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6541 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6601 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga
     6661 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6721 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt ccggaaatac
     6781 aggaacgcac gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc
     6841 agtggattaa gtgggggtaa tgtggcctgt accctctggt tgcataggta ttcatacggt
     6901 taaaatttat caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca tttttacctg
     6961 tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag ggattatggt
     7021 aaatccactt actgtctgcc ctcgtagcca tcgagataaa ccgcagtact ccggccacga
     7081 tgcgtccggc gtagaggatc gagatct
//
