LOCUS       Exported                5027 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       UnnamedSequence      5161 bp    DNA   linear            
             9/13/2011

            DEFINITION  Current Name: Unnamed Sequence
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from %22CMV 
                     Promoter%22 to %22CMV immediate-early enhancer/promoter%22 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     misc_feature    1053..1135
                     /label=Untitled
     D-loop          1065..1100
                     /label=smBiT
                     /note="smBiT"
     stem_loop       1101..1130
                     /label=Gly Ser linker
                     /note="Gly Ser linker"
     misc_feature    1131..1138
                     /label=SgfI site
                     /note="used in Flexi cloning"
     misc_feature    1140..1859
                     /label=BCL2 insert
                     /note="Recieved from Kazusa in Nov 2009. Entered into 
                     database semi automatically by Ethan in Jan 2010. (note 
                     added in Ethan Strauss by 2/1/2010 10:15:22 AM"
     misc_feature    1545..1550
                     /label=EcoICR I
     misc_feature    1857..1864
                     /label=PmeI site
                     /note="used in Flexi cloning"
     polyA_signal    2016..2237
                     /label=Sv40 late polyadenylation signal
     promoter        2336..2754
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        2762..2828
                     /label=EM7 bacterial promoter
     CDS             2842..3636
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    3700..3748
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      3984..4020
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    4691..4976
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtg accggctacc
     1081 ggctgttcga ggagattctg ggaggaggtg gctcaggtgg agggggctcc gcgatcgcca
     1141 tggcgcacgc tgggagaaca gggtacgata accgggagat agtgatgaag tacatccatt
     1201 ataagctgtc gcagaggggc tacgagtggg atgcgggaga tgtgggcgcc gcgcccccgg
     1261 gggccgcccc cgcaccgggc atcttctcct cccagcccgg gcacacgccc catccagccg
     1321 catcccggga cccggtcgcc aggacctcgc cgctgcagac cccggctgcc cccggcgccg
     1381 ccgcggggcc tgcgctcagc ccggtgccac ctgtggtcca cctgaccctc cgccaggccg
     1441 gcgacgactt ctcccgccgc taccgccgcg acttcgccga gatgtccagc cagctgcacc
     1501 tgacgccctt caccgcgcgg ggacgctttg ccacggtggt ggaggagctc ttcagggacg
     1561 gggtgaactg ggggaggatt gtggccttct ttgagttcgg tggggtcatg tgtgtggaga
     1621 gcgtcaaccg ggagatgtcg cccctggtgg acaacatcgc cctgtggatg actgagtacc
     1681 tgaaccggca cctgcacacc tggatccagg ataacggagg ctgggatgcc tttgtggaac
     1741 tgtacggccc cagcatgcgg cctctgtttg atttctcctg gctgtctctg aagactctgc
     1801 tcagtttggc cctggtggga gcttgcatca ccctgggtgc ctatctgggc cacaaggttt
     1861 aaacgaattc gggctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     1921 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     1981 taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat acattgatga
     2041 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2101 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2161 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     2221 cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat
     2281 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     2341 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     2401 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     2461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     2521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     2581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2701 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaattaa
     2761 ctgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     2821 actaaaccca ggaggcagat catgattgaa caagatggat tgcacgcagg ttctccggcc
     2881 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2941 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3001 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     3061 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     3121 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     3181 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3241 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3301 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     3361 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     3421 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3481 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3541 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3601 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     3661 ccgaccaagc gacgcccaac ctgccatcac gatggccgca ataaaatatc tttattttca
     3721 ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt
     3781 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     3841 tgcggactgg ctttctaccc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3901 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3961 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4021 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4081 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4141 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4201 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4261 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4321 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4381 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4441 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4501 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4561 aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4621 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4681 cttttatagt ccggaaatac aggaacgcac gctggatggc ccttcgctgg gatggtgaaa
     4741 ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa tgtggcctgt accctctggt
     4801 tgcataggta ttcatacggt taaaatttat caggcgcgat tgcggcagtt tttcgggtgg
     4861 tttgttgcca tttttacctg tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg
     4921 tacaattaag ggattatggt aaatccactt actgtctgcc ctcgtagcca tcgagataaa
     4981 ccgcagtact ccggccacga tgcgtccggc gtagaggatc gagatct
//
