LOCUS       Exported                6367 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pNB189

            

            Digest ATG1080 with Sgf1/Pme1.  Gel purify 2339 bp fragement and 
            C-terminal transfer into pNB-Flexi-2
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..753
                     /label=HSV-TK
                     /note="HSV-TK"
     misc_feature    1..21
                     /label=NB76
                     /note="NB76"
     misc_feature    complement(766..794)
                     /label=NB77
                     /note="NB77"
     misc_feature    793..797
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    798..3232
                     /label=Untitled
     CDS             802..3132
                     /label=GR
                     /note="NR3C1 sequence"
     CDS             3142..3186
                     /codon_start=1
                     /label=15GS linker
                     /note="15GS linker"
                     /translation="GSSGGGGSGGGGSSG"
     CDS             3187..3219
                     /codon_start=1
                     /label=114 complementation peptide
                     /note="114 complementation peptide"
                     /translation="VTGYRLFEEIL"
     polyA_signal    3356..3577
                     /label=Sv40 late polyadenylation signal
     promoter        3676..4094
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(3749..3985)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      3992..4057
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        4102..4168
                     /label=EM7 bacterial promoter
     CDS             4182..4976
                     /label=NeoR / KanR
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    5040..5088
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5324..5360
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6031..6316
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 aaatgagtct tcggacctcg cgggggccgc ttaagcggtg gttagggttt gtctgacgcg
       61 gggggagggg gaaggaacga aacactctca ttcggaggcg gctcggggtt tggtcttggt
      121 ggccacgggc acgcagaaga gcgccgcgat cctcttaagc acccccccgc cctccgtgga
      181 ggcgggggtt tggtcggcgg gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc
      241 cccagagtgt gaccttttcg gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg
      301 acgggctcgc tgggtcctag gctccatggg gaccgtatac gtggacaggc tctggagcat
      361 ccgcacgact gcggtgatat taccggagac cttctgcggg acgagccggg tcacgcggct
      421 gacgcggagc gtccgttggg cgacaaacac caggacgggg cacaggtaca ctatcttgtc
      481 acccggaggc gcgagggact gcaggagctt cagggagtgg cgcagctgct tcatccccgt
      541 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
      601 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaagtcga acacgcagat
      661 gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
      721 gaacaccgag cgaccctgca gcgacccgct taaaagcttg gcaatccggt actgttggta
      781 aagccaccgc tagcgatcgc catggactcc aaagaatcat taactcctgg tagagaagaa
      841 aaccccagca gtgtgcttgc tcaggagagg ggagatgtga tggacttcta taaaacccta
      901 agaggaggag ctactgtgaa ggtttctgcg tcttcaccct cactggctgt cgcttctcaa
      961 tcagactcca agcagcgaag acttttggtt gattttccaa aaggctcagt aagcaatgcg
     1021 cagcagccag atctgtccaa agcagtttca ctctcaatgg gactgtatat gggagagaca
     1081 gaaacaaaag tgatgggaaa tgacctggga ttcccacagc agggccaaat cagcctttcc
     1141 tcgggggaaa cagacttaaa gcttttggaa gaaagcattg caaacctcaa taggtcgacc
     1201 agtgttccag agaaccccaa gagttcagca tccactgctg tgtctgctgc ccccacagag
     1261 aaggagtttc caaaaactca ctctgatgta tcttcagaac agcaacattt gaagggccag
     1321 actggcacca acggtggcaa tgtgaaattg tataccacag accaaagcac ctttgacatt
     1381 ttgcaggatt tggagttttc ttctgggtcc ccaggtaaag agacgaatga gagtccttgg
     1441 agatcagacc tgttgataga tgaaaactgt ttgctttctc ctctggcggg agaagacgat
     1501 tcattccttt tggaaggaaa ctcgaatgag gactgcaagc ctctcatttt accggacact
     1561 aaacccaaaa ttaaggataa tggagatctg gttttgtcaa gccccagtaa tgtaacactg
     1621 ccccaagtga aaacagaaaa agaagatttc atcgaactct gcacccctgg ggtaattaag
     1681 caagagaaac tgggcacagt ttactgtcag gcaagctttc ctggagcaaa tataattggt
     1741 aataaaatgt ctgccatttc tgttcatggt gtgagtacct ctggaggaca gatgtaccac
     1801 tatgacatga atacagcatc cctttctcaa cagcaggatc agaagcctat ttttaatgtc
     1861 attccaccaa ttcccgttgg ttccgaaaat tggaataggt gccaaggatc tggagatgac
     1921 aacttgactt ctctggggac tctgaacttc cctggtcgaa cagttttttc taatggctat
     1981 tcaagcccca gcatgagacc agatgtaagc tctcctccat ccagctcctc aacagcaaca
     2041 acaggaccac ctcccaaact ctgcctggtg tgctctgatg aagcttcagg atgtcattat
     2101 ggagtcttaa cttgtggaag ctgtaaagtt ttcttcaaaa gagcagtgga aggacagcac
     2161 aattacctat gtgctggaag gaatgattgc atcatcgata aaattcgaag aaaaaactgc
     2221 ccagcatgcc gctatcgaaa atgtcttcag gctggaatga acctggaagc tcgaaaaaca
     2281 aagaaaaaaa taaaaggaat tcagcaggcc actacaggag tctcacaaga aacctctgaa
     2341 aatcctggta acaaaacaat agttcctgca acgttaccac aactcacccc taccctggtg
     2401 tcactgttgg aggttattga acctgaagtg ttatatgcag gatatgatag ctctgttcca
     2461 gactcaactt ggaggatcat gactacgctc aacatgttag gagggcggca agtgattgca
     2521 gcagtgaaat gggcaaaggc aataccaggt ttcaggaact tacacctgga tgaccaaatg
     2581 accctactgc agtactcctg gatgtttctt atggcatttg ctctggggtg gagatcatat
     2641 agacaatcaa gtgcaaacct gctgtgtttt gctcctgatc tgattattaa tgagcagaga
     2701 atgactctac cctgcatgta cgaccaatgt aaacacatgc tgtatgtttc ctctgagtta
     2761 cacaggcttc aggtatctta tgaagagtat ctctgtatga aaaccttact gcttctctct
     2821 tcagttccta aggacggtct gaagagccaa gagctatttg atgaaattag aatgacctac
     2881 atcaaagagc taggaaaagc cattgtcaag agggaaggaa actccagcca gaactggcag
     2941 cggttttatc aactgacaaa actcttggat tctatgcatg aagtggttga aaatctcctt
     3001 aactattgct tccaaacatt tttggataag accatgagta ttgaattccc cgagatgtta
     3061 gctgaaatca tcaccaatca gataccaaaa tattcaaatg gaaatatcaa aaaacttctg
     3121 tttcatcaaa aggtttctca aggcagttca ggtggtggcg ggagcggagg tggaggctcg
     3181 agcggtgtga ccggctaccg gctgttcgag gagattctgt aaggccgcga ctctagagtc
     3241 gacctgcagg catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     3301 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac
     3361 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     3421 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3481 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     3541 gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt
     3601 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3661 cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     3721 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     3781 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3841 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3901 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3961 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4081 tttgcaaaaa gcttaattaa ctgttgacaa ttaatcatcg gcatagtata tcggcatagt
     4141 ataatacgac aaggtgagga actaaaccca ggaggcagat catgattgaa caagatggat
     4201 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4261 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4321 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     4381 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4441 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4501 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4561 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4621 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4681 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     4741 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4801 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4861 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4921 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     4981 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca
     5041 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga
     5101 taaggatcct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca
     5161 tccggggtca gcaccgtttc tgcggactgg ctttctaccc ggtatcagct cactcaaagg
     5221 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5281 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5521 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5761 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5821 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5881 agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg
     5941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6001 aaaggatctt cacctagatc cttttatagt ccggaaatac aggaacgcac gctggatggc
     6061 ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa
     6121 tgtggcctgt accctctggt tgcataggta ttcatacggt taaaatttat caggcgcgat
     6181 tgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgct
     6241 gaacgcgttt tagcggtgcg tacaattaag ggattatggt aaatccactt actgtctgcc
     6301 ctcgtagcca tcgagataaa ccgcagtact ccggccacga tgcgtccggc gtagaggatc
     6361 gagatct
//
