LOCUS       DSTYK-31Kp              7420 bp ds-DNA     circular     11-JUL-2022
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: DSTYK-31Kp.gb
            Current Name: pF-31Kp - DSTYK Sequence 'pF-31Kp-DSTYK' generated by
            SeqNinja on Jun 22, 2022 at 10:11:08 AM CDT. Equivalent SeqNinja
            expression: "G:\Public\1810DFK Investigation\Plasmids to Upload to
            Benchling\A Kinases\DSTYK-31Kp.sbd"#"pF-31Kp-DSTYK"
FEATURES             Location/Qualifiers
     source          1..7420
                     /label="source"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        1..742
                     /label="CMV immediate-early enhancer/promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="note: Has EcoICRI site removed. Named changed from """"CMVPromoter"""" to """"CMV immediate-early enhancer/promoter""""3/21/2011 and J Hartnet's request to fit Sci Com'snaming. dnas_type: CMV immediate-early enhancer/promoter"
     intron          857..989
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: Note that this is the actual sequence spliced out.The 5' donor site includes a 5' GA and the 3' acceptorsite adds a 3'G. dnas_type: chimeric intron"
     promoter        1033..1052
                     /label="T7 RNA Polymerase promoter (-17 to +3)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="dnas_type: T7 RNA Polymerase promoter"
     CDS             1065..1577
                     /label="N terminal NanoLuc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="dnas_type: N terminal NanoLuc"
     CDS             1065..1577
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA"
     CDS             1065..4391
                     /label="Complete ORF (with fusions) of DSTYK"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: This insert contains an Sgf I site at position 0of the unmodified insert. This insert contains a Pme Isite at position 2796 of the unmodified insert. dnas_type: Complete ORF"
     CDS             1065..4391
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILAGSSGAIAMEGDGVPWGSEPVSGPGPGGGGMIRELCRGFGRYRRYLGRLRQNLRETQKFFRDIKCSHNHTCLSSLTGGGGAERGPAGDVAETGLQAGQLSCISFPPKEEKYLQQIVDCLPCILILGQDCNVKCQLLNLLLGVQVLPTTKLGSEESCKLRRLRFTYGTQTRVSLALPGQYELVHTLVAHQGNWETIPEEDLEVQENNEDAAHVLAELEVTMHHALLQEVDVVVAPCQGLRPTVDVLGDLVNDFLPVITYALHKDELSERDEQELQEIRKYFSFPVFFFKVPKLGSEIIDSSTRRMESERSPLYRQLIDLGYLSSSHWNCGAPGQDTKAQSMLVEQSEKLRHLSTFSHQVLQTRLVDAAKALNLVHCHCLDIFINQAFDMQRDLQITPKRLEYTRKKENELYESLMNIANRKQEEMKDMIVETLNTMKEELLDDATNMEFKDVIVPENGEPVGTREIKCCIRQIQELIISRLNQAVANKLISSVDYLRESFVGTLERCLQSLEKSQDVSVHITSNYLKQILNAAYHVEVTFHSGSSVTRMLWEQIKQIIQRITWVSPPAITLEWKRKVAQEAIESLSASKLAKSICSQFRTRLNSSHEAFAASLRQLEAGHSGRLEKTEDLWLRVRKDHAPRLARLSLESCSLQDVLLHRKPKLGQELGRGQYGVVYLCDNWGGHFPCALKSVVPPDEKHWNDLALEFHYMRSLPKHERLVDLHGSVIDYNYGGGSSIAVLLIMERLHRDLYTGLKAGLTLETRLQIALDVVEGIRFLHSQGLVHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPELFTGKYDNSVDVYAFGILFWYICSGSVKLPEAFERCASKDHLWNNVRRGARPERLPVFDEECWQLMEACWDGDPLKRPLLGIVQPMLQGIMNRLCKSNSEQPNRGLDDSTV*"
     misc_feature    1578..1589
                     /label="N Terminal NanoLuc linker"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="dnas_type: N Terminal NanoLuc linker"
     misc_feature    1590..1597
                     /label="SgfI Site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: Used in Flexi Vector Cloning dnas_type: SgfI Site"
     misc_feature    1594..4389
                     /label="DSTYK insert"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: This insert contains an Sgf I site at position 0of the unmodified insert. This insert contains a Pme Isite at position 2796 of the unmodified insert. dnas_type: DSTYK insert"
     misc_feature    4386..4393
                     /label="PmeI site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: Used in Flexi Vector Cloning dnas_type: PmeI site"
     misc_feature    4390..4523
                     /label="Transfer Efficiency Cassette"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: Feature changed from""""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGC"""" at Jim Hartnet's request12/18/2007 to include the complete cassette. dnas_type: Transfer Efficiency Cassette"
     polyA_signal    4545..4766
                     /label="Sv40 late polyadenylation signal"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="dnas_type: Sv40 late polyadenylation signal"
     promoter        4865..5283
                     /label="SV40 enhancer and early promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: Name changed frm """"SV40 early enhancer/promoterregion"""" to """"SV40 enhancer and early promoter"""" on3/21/2011 at Jim Hartnet's request to fit Sci Com'snaming. dnas_type: SV40 enhancer and early promoter"
     enhancer        complement(4938..5174)
                     /label="SV40 Enhancer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: Sequence shown is from SV40 genome. Our vectorscontain sequences with one or two point mutations infirst few bases. These apparently do not affect function.The sequences present in our vectors are shown to theright. dnas_type: SV40 Enhancer"
     rep_origin      5181..5246
                     /label="Sv40 Min Ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="dnas_type: Sv40 Min Ori"
     promoter        5291..5357
                     /label="EM7 bacterial promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="dnas_type: EM7 bacterial promoter"
     CDS             5371..6165
                     /label="Neomycin phosphotransferase coding region"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="note: Name changed frm """"Neomycin phosphotransferase""""to """"Neomycin phosphotransferase coding region"""" on3/21/2011 at Jim Hartnet's request to fit Sci Com'snaming. dnas_type: Neomycin phosphotransferase coding"
     CDS             5371..6165
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     polyA_signal    6229..6277
                     /label="Synthetic PolyAdenylation Signal Sequence (frompCiNeo vectors)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="dnas_type: Synthetic PolyAdenylation Signal"
ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC
      661 CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC
      721 TGGTTTAGTG AACCGTCAGA TCACTAGAAG CTTTATTGCG GTAGTTTATC ACAGTTAAAT
      781 TGCTAACGCA GTCAGTGCTT CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC
      841 GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA
      901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC
      961 TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA
     1021 AGGCTAGAGT ATTAATACGA CTCACTATAG GGCTAGCGCT CACCATGGTC TTCACACTCG
     1081 AAGATTTCGT TGGGGACTGG CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC
     1141 AGGGAGGTGT GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA
     1201 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG
     1261 GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT TAAGGTGGTG TACCCTGTGG
     1321 ATGATCATCA CTTTAAGGTG ATCCTGCACT ATGGCACACT GGTAATCGAC GGGGTTACGC
     1381 CGAACATGAT CGACTATTTC GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA
     1441 AGATCACTGT AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA
     1501 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC TGGCGGCTGT
     1561 GCGAACGCAT TCTGGCGGGC TCGAGCGGCG CGATCGCCAT GGAGGGCGAC GGCGTGCCCT
     1621 GGGGCAGCGA GCCCGTGAGC GGACCAGGAC CTGGAGGAGG CGGCATGATC CGGGAGCTGT
     1681 GCAGAGGCTT TGGCAGGTAC CGGAGATATC TGGGCCGGCT GAGACAGAAC CTGCGCGAGA
     1741 CCCAGAAGTT CTTTCGGGAC ATCAAGTGCA GCCACAATCA CACCTGTCTG AGCAGCCTGA
     1801 CAGGAGGAGG AGGAGCAGAG AGGGGACCAG CAGGCGATGT GGCCGAGACA GGCCTGCAGG
     1861 CCGGCCAGCT GTCTTGCATC AGCTTTCCCC CTAAGGAGGA GAAGTACCTG CAGCAGATCG
     1921 TGGACTGCCT GCCCTGTATC CTGATCCTGG GCCAGGATTG CAACGTGAAG TGTCAGCTGC
     1981 TGAATCTGCT GCTGGGCGTG CAGGTGCTGC CTACCACAAA GCTGGGCTCC GAGGAGTCTT
     2041 GTAAGCTGAG GCGCCTGAGA TTCACCTACG GCACCCAGAC AAGGGTGTCC CTGGCCCTGC
     2101 CAGGACAGTA TGAGCTGGTG CACACCCTGG TGGCACACCA GGGCAACTGG GAGACAATCC
     2161 CTGAGGAGGA TCTGGAGGTG CAGGAGAACA ATGAGGACGC AGCACACGTG CTGGCAGAGC
     2221 TGGAGGTGAC CATGCACCAC GCCCTGCTGC AGGAGGTGGA CGTGGTGGTG GCACCATGCC
     2281 AGGGACTGAG GCCTACCGTG GACGTGCTGG GCGATCTGGT GAATGACTTC CTGCCCGTGA
     2341 TCACATACGC CCTGCACAAG GATGAGCTGA GCGAGCGCGA CGAGCAGGAG CTGCAGGAGA
     2401 TCCGGAAGTA CTTCAGCTTC CCAGTGTTCT TTTTCAAGGT GCCCAAGCTG GGCTCCGAGA
     2461 TCATCGACTC TAGCACCCGG AGAATGGAGA GCGAGCGGAG CCCACTGTAC CGGCAGCTGA
     2521 TCGACCTGGG CTATCTGTCC TCTAGCCACT GGAACTGTGG CGCCCCTGGA CAGGATACAA
     2581 AGGCCCAGTC TATGCTGGTG GAGCAGAGCG AGAAGCTGAG ACACCTGTCT ACCTTTAGCC
     2641 ACCAGGTGCT GCAGACAAGG CTGGTGGACG CAGCAAAGGC CCTGAACCTG GTGCACTGCC
     2701 ACTGTCTGGA TATCTTTATC AATCAGGCCT TCGATATGCA GCGCGACCTG CAGATCACCC
     2761 CTAAGAGACT GGAGTACACA AGGAAGAAGG AGAACGAGCT GTATGAGTCC CTGATGAACA
     2821 TCGCCAATCG GAAGCAGGAG GAGATGAAGG ACATGATCGT GGAGACCCTG AATACAATGA
     2881 AGGAGGAGCT GCTGGACGAT GCCACCAACA TGGAGTTCAA GGATGTGATC GTGCCAGAGA
     2941 ATGGCGAGCC CGTGGGCACA AGAGAGATCA AGTGCTGTAT CAGGCAGATC CAGGAGCTGA
     3001 TCATCAGCAG ACTGAACCAG GCCGTGGCCA ATAAGCTGAT CTCCTCTGTG GACTACCTGA
     3061 GAGAGTCCTT TGTGGGCACC CTGGAGAGGT GCCTGCAGTC TCTGGAGAAG AGCCAGGACG
     3121 TGAGCGTGCA CATCACATCC AACTACCTGA AGCAGATCCT GAATGCCGCC TATCACGTGG
     3181 AGGTGACCTT CCACTCTGGC AGCTCCGTGA CACGCATGCT GTGGGAGCAG ATCAAGCAGA
     3241 TCATCCAGAG GATCACCTGG GTGAGCCCAC CAGCCATCAC ACTGGAGTGG AAGAGGAAGG
     3301 TGGCACAGGA GGCAATCGAG AGCCTGTCCG CCTCTAAGCT GGCCAAGTCC ATCTGCTCTC
     3361 AGTTTAGGAC CCGCCTGAAC TCTAGCCACG AGGCCTTCGC CGCATCCCTG AGGCAGCTGG
     3421 AGGCAGGACA CTCTGGCCGG CTGGAGAAGA CAGAGGATCT GTGGCTGCGG GTGAGAAAGG
     3481 ACCACGCACC TAGGCTGGCA AGGCTGTCCC TGGAGAGCTG TTCCCTGCAG GACGTGCTGC
     3541 TGCACAGAAA GCCAAAGCTG GGACAGGAGC TGGGAAGGGG ACAGTACGGC GTGGTGTATC
     3601 TGTGCGATAA CTGGGGCGGC CACTTTCCAT GTGCCCTGAA GTCTGTGGTG CCTCCAGATG
     3661 AGAAGCACTG GAATGACCTG GCCCTGGAGT TCCACTACAT GCGCTCTCTG CCCAAGCACG
     3721 AGCGGCTGGT GGATCTGCAC GGCAGCGTGA TCGACTACAA TTATGGAGGA GGCTCCTCTA
     3781 TCGCCGTGCT GCTGATCATG GAGCGGCTGC ACAGAGACCT GTATACCGGC CTGAAGGCAG
     3841 GACTGACCCT GGAGACAAGA CTGCAGATCG CCCTGGATGT GGTGGAGGGC ATCCGCTTCC
     3901 TGCACTCCCA GGGACTGGTG CACAGGGATA TCAAGCTGAA GAACGTGCTG CTGGACAAGC
     3961 AGAATAGGGC CAAGATCACC GATCTGGGCT TTTGCAAGCC TGAGGCCATG ATGTCTGGCA
     4021 GCATCGTGGG CACCCCTATC CACATGGCCC CAGAGCTGTT CACAGGCAAG TACGATAACT
     4081 CTGTGGACGT GTATGCCTTT GGCATCCTGT TCTGGTACAT CTGCTCCGGC TCTGTGAAGC
     4141 TGCCAGAGGC CTTCGAGCGG TGCGCAAGCA AGGACCACCT GTGGAACAAT GTGAGGAGGG
     4201 GCGCCAGGCC AGAGCGCCTG CCCGTGTTCG ATGAGGAGTG CTGGCAGCTG ATGGAGGCCT
     4261 GTTGGGACGG CGATCCTCTG AAGCGGCCAC TGCTGGGCAT CGTGCAGCCT ATGCTGCAGG
     4321 GCATCATGAA TAGACTGTGC AAGAGCAACT CCGAGCAGCC AAATAGGGGC CTGGACGATA
     4381 GCACCGTTTA AACGAATTCG GGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC
     4441 ATGCAAGCTG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC
     4501 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCGGCCGCTT CGAGCAGACA TGATAAGATA
     4561 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA
     4621 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA
     4681 CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG ATGTGGGAGG TTTTTTTAAG
     4741 CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGAATTTTAA CAAAATATTA ACGCTTACAA
     4801 TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGCGG
     4861 ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG GAACTTGGTT AGGTACCTTC
     4921 TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC
     4981 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA
     5041 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA
     5101 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT
     5161 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC
     5221 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
     5281 CTTAATTAAC TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
     5341 AGGTGAGGAA CTAAACCCAG GAGGCAGATC ATGATTGAAC AAGATGGATT GCACGCAGGT
     5401 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
     5461 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
     5521 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
     5581 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
     5641 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
     5701 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
     5761 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
     5821 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
     5881 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT
     5941 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
     6001 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
     6061 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
     6121 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT
     6181 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG ATGGCCGCAA TAAAATATCT
     6241 TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAAT CGATAGCGAT AAGGATCCGC
     6301 TCATGCGATG ATAAGCTGTC AAACATGAGA ATTACAACTT ATATCGTATG GGGCTGACTT
     6361 CAGGTGCTAC ATTTGAAGAG ATAAATTGCA CTGAAATCTA GAAATATTTT ATCTGATTAA
     6421 TAAGATGATC TTCTTGAGAT CGTTTTGGTC TGCGCGTAAT CTCTTGCTCT GAAAACGAAA
     6481 AAACCGCCTT GCAGGGCGGT TTTTCGAAGG TTCTCTGAGC TACCAACTCT TTGAACCGAG
     6541 GTAACTGGCT TGGAGGAGCG CAGTCACCAA AACTTGTCCT TTCAGTTTAG CCTTAACCGG
     6601 CGCATGACTT CAAGACTAAC TCCTCTAAAT CAATTACCAG TGGCTGCTGC CAGTGGTGCT
     6661 TTTGCATGTC TTTCCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG
     6721 GACTGAACGG GGGGTTCGTG CATACAGTCC AGCTTGGAGC GAACTGCCTA CCCGGAACTG
     6781 AGTGTCAGGC GTGGAATGAG ACAAACGCGG CCATAACAGC GGAATGACAC CGGTAAACCG
     6841 AAAGGCAGGA ACAGGAGAGC GCACGAGGGA GCCGCCAGGG GGAAACGCCT GGTATCTTTA
     6901 TAGTCCTGTC GGGTTTCGCC ACCACTGATT TGAGCGTCAG ATTTCGTGAT GCTTGTCAGG
     6961 GGGGCGGAGC CTATGGAAAA ACGGCTTTGC CGCGGCCCTC TCACTTCCCT GTTAAGTATC
     7021 TTCCTGGCAT CTTCCAGGAA ATCTCCGCCC CGTTCGTAAG CCATTTCCGC TCGCCGCAGT
     7081 CGAACGACCG AGCGTAGCGA GTCAGTGAGC GAGGAAGCGG AATATATCCT GTATCACATA
     7141 TTCTGCTGAC GCACCGGTGC AGCCTTTTTT CTCCTGCCAC ATGAAGCACT TCACTGACAC
     7201 CCTCATCAGT GCCAACATAG TAAGCCAGTA TACACTCCGC TAGCGCTGAT GTCCGGCGGT
     7261 GCTTTTGCCG TTACGCACCA CCCCGTCATG AGATTACGCG TTTTAGCGGT GCGTACAATT
     7321 AAGGGATTAT GGTAAATCCA CTTACTGTCT GCCCTCGTAG CCATCGAGAT AAACCGCAGT
     7381 ACTCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT
//