LOCUS       Exported                7019 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-32Kp-PIK3C2B      9533 bp    DNA   circular          
             5/18/2017

            DEFINITION  Current Name: pF-32Kp - PIK3C2B
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..3476
                     /label=DGKb Uniprot Q9Y6T7-1 XM_011515154.2
                     /note="DGKb Uniprot Q9Y6T7-1 XM_011515154.2"
     misc_feature    3477..3483
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    3484..3497
                     /label=C Terminal NanoLuc linker
     CDS             3498..4007
                     /label=C terminal NanoLuc
     polyA_signal    4144..4365
                     /label=Sv40 late polyadenylation signal
     promoter        4464..4882
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4890..4956
                     /label=EM7 bacterial promoter
     CDS             4970..5764
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5828..5876
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgaca aaccaggaaa
     1081 aatgggccca cctcagccct tcggaatttt cccaacttca gaaatatgct gagtattcta
     1141 caaagaaatt aaaggatgtt cttgaagaat tccatggtaa tggtgtgctt gcaaagtata
     1201 atcctgaagg gaaacaagac attcttaacc aaacaataga ttttgaaggt ttcaaactat
     1261 tcatgaagac attcctggaa gccgagcttc ctgatgattt cactgcacac cttttcatgt
     1321 catttagcaa caagtttcct cattctagtc caatggtaaa aagtaagcct gctctcctat
     1381 caggcggtct gagaatgaat aaaggtgcca tcacccctcc ccggactact tctcctgcaa
     1441 atacgtgttc cccagaagta atccatctga aggacattgt ctgttacctg tctctgcttg
     1501 aaagaggaag acctgaggat aagcttgagt ttatgtttcg cctttatgac acggatggga
     1561 atggcttcct ggacagctcg gagctagaaa atatcatcag tcagatgatg catgttgcag
     1621 aataccttga gtgggatgtc actgaactta atccaatcct ccatgaaatg atggaagaaa
     1681 ttgactatga tcatgatgga accgtgtctc tggaggaatg gattcaagga ggaatgacaa
     1741 cgattccact tcttgtgctc ctgggcttag aaaataacgt gaaggatgat ggacagcacg
     1801 tgtggcgact gaagcacttt aacaaacctg cctattgcaa cctttgcctg aacatgctga
     1861 ttggcgtggg gaagcagggc ctctgctgtt ccttctgcaa gtacacagtc catgagcgct
     1921 gtgtggctcg agcacctccc tcttgcatca agacctatgt gaagtccaaa aggaacactg
     1981 atgtcatgca ccattactgg gttgaaggta actgcccaac caagtgtgat aagtgccaca
     2041 aaactgttaa atgttaccag ggcctgacag gactgcattg tgtttggtgt cagatcacac
     2101 tgcataataa atgtgcttct catctaaaac ctgaatgtga ctgtggacct ttgaaggacc
     2161 atattttacc acccacaaca atctgtccag tggtactgca gactctgccc acttcaggag
     2221 tttcagttcc tgaggaaaga caatcaacag tgaaaaagga aaagagtggt tcccagcagc
     2281 caaacaaagt gattgacaag aataaaatgc aaagagccaa ctctgttact gtagatggac
     2341 aaggcctgca ggtcactcct gtgcctggta ctcacccact tttagttttt gtgaacccca
     2401 aaagtggtgg aaaacaagga gaacgaattt acagaaaatt ccagtatcta ttaaatcctc
     2461 gtcaggttta cagtctttct ggaaatggac caatgccagg gttaaacttt ttccgtgatg
     2521 ttcctgactt cagagtgtta gcctgtggtg gagatggaac cgtgggctgg gttttggatt
     2581 gcatagaaaa ggccaatgta ggcaagcatc ctccagttgc gattctgcct cttgggactg
     2641 gcaatgatct agcaagatgc ctgcgatggg gaggaggtta cgaaggtgag aatctgatga
     2701 aaattctaaa agacattgaa aacagcacag aaatcatgtt ggacaggtgg aagtttgaag
     2761 tcatacctaa tgacaaagat gagaaaggag acccagtgcc ttacagtatc atcaataatt
     2821 acttttccat tggcgtggat gcctccattg cacacagatt ccacatcatg agagaaaaac
     2881 acccagagaa attcaacagt agaatgaaga acaaattttg gtattttgag tttggcacat
     2941 ctgaaacttt ctcagccacc tgcaagaagc tacatgaatc tgtagaaata gaatgtgatg
     3001 gagtacagat agatttaata aacatctctc tggaaggaat tgctattttg aatataccaa
     3061 gcatgcatgg aggatccaat ctttggggag agtctaagaa aagacgaagc catcgacgaa
     3121 tagagaaaaa agggtctgac aaaaggacca ccgtcacaga tgccaaagag ttgaagtttg
     3181 caagtcaaga tctcagtgac cagctgctgg aggtggtcgg cttggaagga gccatggaga
     3241 tggggcaaat atacacaggc ctgaaaagtg ctggccggcg gctggctcag tgctcctgcg
     3301 tggtcatcag gacgagcaag tctctgccaa tgcaaattga tggggagcca tggatgcaga
     3361 ccccatgcac aataaaaatt acacacaaga accaagcccc aatgctgatg ggcccgcctc
     3421 caaaaaccgg tttattctgc tccctcgtca aaaggacaag aaaccgaagc aaggaagttt
     3481 ctctcggctc gagcggcgtc ttcacactcg aagatttcgt tggggactgg cgacagacag
     3541 ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc
     3601 tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga
     3661 tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg
     3721 aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact
     3781 atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt
     3841 atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg
     3901 gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa
     3961 ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa ggccgcgact
     4021 ctagagtcga cctgcaggca tgcaagctga tccggctgct aacaaagccc gaaaggaagc
     4081 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cggccgcttc
     4141 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     4201 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     4261 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     4321 tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc gaattttaac
     4381 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4441 tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     4501 aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     4561 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4621 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4681 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4741 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4801 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4861 cctaggcttt tgcaaaaagc ttaattaact gttgacaatt aatcatcggc atagtatatc
     4921 ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca tgattgaaca
     4981 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5041 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5101 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5161 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5221 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5281 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5341 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5401 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5461 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5521 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5581 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5641 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5701 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5761 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5821 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     5881 gatagcgata aggatccgct catgcgatga taagctgtca aacatgagaa ttacaactta
     5941 tatcgtatgg ggctgacttc aggtgctaca tttgaagaga taaattgcac tgaaatctag
     6001 aaatatttta tctgattaat aagatgatct tcttgagatc gttttggtct gcgcgtaatc
     6061 tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt tctctgagct
     6121 accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa acttgtcctt
     6181 tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc aattaccagt
     6241 ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc
     6301 ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     6361 aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg
     6421 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     6541 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc gcggccctct
     6601 cacttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc
     6661 catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     6721 atatatcctg tatcacatat tctgctgacg caccggtgca gccttttttc tcctgccaca
     6781 tgaagcactt cactgacacc ctcatcagtg ccaacatagt aagccagtat acactccgct
     6841 agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcatga gattacgcgt
     6901 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
     6961 catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatct
//
