LOCUS       Exported                6980 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-32Kp-PIK3C2B      9533 bp    DNA   circular          
             5/18/2017

            DEFINITION  Current Name: pF-32Kp - PIK3C2B
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..3437
                     /label=DGKg Uniprot P49619-1 NM_001346.2
                     /note="DGKg Uniprot P49619-1 NM_001346.2"
     misc_feature    3438..3444
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    3445..3458
                     /label=C Terminal NanoLuc linker
     CDS             3459..3968
                     /label=C terminal NanoLuc
     polyA_signal    4105..4326
                     /label=Sv40 late polyadenylation signal
     promoter        4425..4843
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4851..4917
                     /label=EM7 bacterial promoter
     CDS             4931..5725
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5789..5837
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgggt gaagaacggt
     1081 gggtctccct cactccagaa gaatttgacc aactccagaa atattcagaa tattcctcca
     1141 agaagataaa agatgccttg actgaattta atgagggtgg gagcctcaaa caatatgacc
     1201 cacatgagcc gattagctat gatgtcttca agctgttcat gagggcgtac ctggaggtgg
     1261 accttcccca gccactgagc actcacctct tcctggcctt cagccagaag cccagacacg
     1321 agacctctga ccacccgacg gagggagcca gcaacagtga ggccaacagc gcagatacta
     1381 atatacagaa tgcagataat gccaccaaag cagacgaggc ctgtgcccct gatactgaat
     1441 caaatatggc tgagaagcaa gcaccagctg aagaccaagt ggctgcgacc cccctggaac
     1501 cccccgtccc tcggtcttca agctcggaat ccccagtggt atacctgaag gatgttgtgt
     1561 gctacctgtc cctgctggag acggggaggc ctcaggataa gctggagttc atgtttcgcc
     1621 tctatgattc agatgagaac ggtctcctgg accaagcgga gatggattgc attgtcaacc
     1681 aaatgctgca tattgcccag tacctggagt gggatcccac agagctgagg cctatattga
     1741 aggagatgct gcaagggatg gactacgacc gggacggctt tgtgtctcta caggaatggg
     1801 tccatggagg gatgaccacc atcccattgc tggtcctcct ggggatggat gactctggct
     1861 ccaaggggga tgggcggcac gcctggacca tgaagcactt caagaaacca acctactgca
     1921 acttctgcca tatcatgctc atgggcgtcc gcaagcaagg cctgtgctgc acttactgta
     1981 aatacactgt ccacgaacgc tgtgtgtcca gaaacattcc tggttgtgtc aaaacgtact
     2041 caaaagccaa aaggagtggt gaggtgatgc agcacgcatg ggtggaaggg aactcctccg
     2101 tcaagtgtga ccggtgccac aaaagtatca agtgctacca gagtgtcacc gcgcggcact
     2161 gcgtgtggtg ccggatgacg tttcaccgca aatgtgaatt atcaacgttg tgtgacggtg
     2221 gggaactcag agaccacatc ttactgccca cctccatatg ccccatcacc cgggacaggc
     2281 caggtgagaa gtctgatggc tgcgtgtccg ccaagggcga acttgtcatg cagtataaga
     2341 tcatccccac cccgggtacc caccccctgc tggtcttggt gaaccccaag agtggaggga
     2401 gacaaggaga aagaattctt cggaaattcc actatctgct caaccccaaa caagttttca
     2461 acctggacaa tggggggcct actccagggt tgaacttttt ccgtgatact ccagacttcc
     2521 gtgttttggc ctgtggtgga gatgggacag ttggctggat tttggattgc attgataagg
     2581 ccaactttgc aaagcatcca ccagtggctg tcctgcctct tggaacagga aatgaccttg
     2641 cccgttgtct ccgctgggga ggaggttatg aagggggcag cttgacaaaa atcctgaaag
     2701 acattgagca gagccccttg gtgatgctgg accgctggca tctggaagtc atccccagag
     2761 aggaagtgga aaacggggac caggtcccat acagcatcat gaacaactat ttctccattg
     2821 gtgtggacgc ttccattgca cacagattcc atgtgatgag agagaaacat cctgaaaaat
     2881 tcaacagcag gatgaagaac aagctgtggt actttgaatt tggcacctcg gagacttttg
     2941 cagcgacctg caagaaactc cacgaccaca ttgagttgga gtgtgatggg gttggggtgg
     3001 acctgagcaa catcttcctg gaaggcattg ccattctcaa cattcccagc atgtacggag
     3061 gcaccaatct ctggggagaa aacaagaaga accgggctgt gatccgggaa agcaggaagg
     3121 gtgtcactga ccccaaagaa ctgaaattct gcgttcaaga cctcagtgac cagctccttg
     3181 aagtggtggg gctagaagga gccatggaga tggggcagat ctacaccggc ctgaagagtg
     3241 caggcaggag gctggcccag tgcgcctctg tcaccatcag gacaaacaag ctgctgccaa
     3301 tgcaagtgga tggagaaccc tggatgcagc catgttgcac gattaaaatt actcacaaga
     3361 accaagcgcc catgatgatg gggcctcccc agaagagcag cttcttctcg ttgagaagga
     3421 agagccgttc aaaagacgtt tctctcggct cgagcggcgt cttcacactc gaagatttcg
     3481 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
     3541 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
     3601 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
     3661 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
     3721 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
     3781 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
     3841 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
     3901 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
     3961 ttctggcgta aggccgcgac tctagagtcg acctgcaggc atgcaagctg atccggctgc
     4021 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     4081 accccttggg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     4141 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4201 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     4261 tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac ctctacaaat
     4321 gtggtaaaat cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     4381 tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag caccatggcc
     4441 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     4501 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4561 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4621 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4681 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4741 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4801 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttaattaac tgttgacaat
     4861 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaacccag
     4921 gaggcagatc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4981 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5041 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5101 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5161 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5221 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5281 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5341 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5401 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5461 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5521 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5581 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5641 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5701 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5761 acgcccaacc tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg
     5821 tgttggtttt ttgtgtgaat cgatagcgat aaggatccgc tcatgcgatg ataagctgtc
     5881 aaacatgaga attacaactt atatcgtatg gggctgactt caggtgctac atttgaagag
     5941 ataaattgca ctgaaatcta gaaatatttt atctgattaa taagatgatc ttcttgagat
     6001 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
     6061 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
     6121 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     6181 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     6241 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
     6301 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     6361 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     6421 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6481 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     6541 acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa
     6601 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
     6661 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc
     6721 agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag
     6781 taagccagta tacactccgc tagcgctgat gtccggcggt gcttttgccg ttacgcacca
     6841 ccccgtcatg agattacgcg ttttagcggt gcgtacaatt aagggattat ggtaaatcca
     6901 cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca cgatgcgtcc
     6961 ggcgtagagg atcgagatct
//
