LOCUS       Exported                5081 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-31Kp-DYRK1A      6922 bp    DNA   circular           
            5/31/2016

            DEFINITION  Current Name: pF-31Kp - DYRK1A
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1590
                     /label=N terminal NanoLuc
     misc_feature    1591..1602
                     /label=N Terminal NanoLuc linker
     misc_feature    1603..1610
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1612..2046
                     /label=TOM20
                     /note="TOM20"
     misc_feature    2047..2054
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2051..2184
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    2206..2427
                     /label=Sv40 late polyadenylation signal
     promoter        2526..2944
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        2952..3018
                     /label=EM7 bacterial promoter
     CDS             3032..3826
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    3890..3938
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgatrtnrts rmnrnmacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg
     1441 ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac
     1501 gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc
     1561 ggctggcggc tgtgcgaacg cattctggcg ggctcgagcg gcgcgatcgc catggttggt
     1621 cgtaattcag ctatagcagc aggagtttgt ggtgcactct ttataggata ctgcatatac
     1681 tttgatcgca aacggcggtc agatccgaat tttaaaaacc gcttgcggga gcgcagaaag
     1741 aagcagaaac tggcaaaaga aagagccggt ctgagtaagc tcccggacct taaagacgct
     1801 gaggcggttc aaaaattttt tttggaggag atccagctcg gggaagaact gttggcccaa
     1861 ggtgaatatg agaaaggtgt tgaccatttg actaacgcaa tagcggtctg cggtcaaccg
     1921 cagcagcttt tgcaagtatt gcagcagacc ctgcctccac ctgtctttca gatgctgctt
     1981 acaaagttgc ctactataag tcagcgaatt gttagtgctc aatcattagc agaagatgat
     2041 gttgaagttt aaacgaattc gggctcggta cccggggatc ctctagagtc gacctgcagg
     2101 catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     2161 cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat
     2221 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2281 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2341 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa
     2401 gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca
     2461 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
     2521 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
     2581 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2761 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2821 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2881 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     2941 gcttaattaa ctgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     3001 aaggtgagga actaaaccca ggaggcagat catgattgaa caagatggat tgcacgcagg
     3061 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3121 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     3181 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     3241 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     3301 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     3361 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     3421 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     3481 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     3541 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     3601 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     3661 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     3721 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     3781 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     3841 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca ataaaatatc
     3901 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatccg
     3961 ctcatgcgat gataagctgt caaacatgag aattacaact tatatcgtat ggggctgact
     4021 tcaggtgcta catttgaaga gataaattgc actgaaatct agaaatattt tatctgatta
     4081 ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa
     4141 aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga
     4201 ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg
     4261 gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc
     4321 ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4381 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     4441 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     4501 gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt
     4561 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     4621 gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat
     4681 cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag
     4741 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat
     4801 attctgctga cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca
     4861 ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctga tgtccggcgg
     4921 tgcttttgcc gttacgcacc accccgtcat gagattacgc gttttagcgg tgcgtacaat
     4981 taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga taaaccgcag
     5041 tactccggcc acgatgcgtc cggcgtagag gatcgagatc t
//
