LOCUS       Exported                5543 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..2498
                     /label=HSD17B13
                     /note="HSD17B13"
     misc_feature    2499..2506
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2658..2879
                     /label=Sv40 late polyadenylation signal
     promoter        2978..3396
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3404..3470
                     /label=EM7 bacterial promoter
     CDS             3484..4278
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4352..4400
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    4443..5365
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    4620
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gaacattatt cttgaaatcc
     1621 ttctccttct gatcactatc atatatagtt accttgaaag tttggtaaaa tttttcattc
     1681 ctcaacggcg gaagtctgtg gccggtgaga tagtcctgat tactggcgca ggacatggaa
     1741 tcggccgcca gacgacgtac gagtttgcta agcgacaatc catactggta ctctgggata
     1801 ttaacaagcg gggtgtagaa gagacagcag ccgagtgtcg caagctgggc gtgactgcac
     1861 acgcatatgt cgtggactgc agtaatcggg aggagattta ccgatccctt aaccaggtaa
     1921 agaaggaggt aggagatgta acaatagttg taaataatgc cggaacggtc tatcctgcgg
     1981 atcttctcag tacaaaggat gaagaaatca caaaaacttt tgaagtgaat atactggggc
     2041 acttctggat aacaaaggcc ctccttccca gtatgatgga acggaaccat ggccacatcg
     2101 taaccgttgc gtccgtgtgc ggacatgagg gcataccata tttgattccc tattgcagca
     2161 gtaagtttgc ggccgtcggt tttcacaggg gattgacaag tgaactgcaa gcgttgggaa
     2221 agacgggtat taaaacctcc tgcctttgtc ccgtgtttgt caacacaggc tttacgaaga
     2281 accccagcac acgattgtgg ccggtgctcg agaccgatga ggtagtgcgg agccttatag
     2341 atggtatcct caccaacaaa aaaatgatct ttgttccctc atatatcaat atttttctgc
     2401 gattgcaaaa atttctgcct gagcgggcta gtgcgatact gaaccggatg caaaacatac
     2461 aattcgaggc ggtcgtcggt cacaagataa agatgaaagt ttaaacgaat tcgggctcgg
     2521 tacccgggga tcctctagag tcgacctgca ggcatgcaag ctgatccggc tgctaacaaa
     2581 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     2641 ggggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2701 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2761 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2821 ggttcagggg gagatgtggg aggttttttt aagcaagtaa aacctctaca aatgtggtaa
     2881 aatcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     2941 gcatctgtgc ggtatttcac accgcatacg cggatctgcg cagcaccatg gcctgaaata
     3001 acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat
     3061 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3121 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3181 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3241 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3301 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3361 ggcttttttg gaggcctagg cttttgcaaa aagcttaatt aactgttgac aattaatcat
     3421 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc caggaggcag
     3481 atcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3541 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3601 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     3661 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     3721 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     3781 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     3841 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     3901 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     3961 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     4021 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4081 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4141 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     4201 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4261 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     4321 acctgccatc acgatggccg dgkgtnrtnm caataaaata tctttatttt cattacatct
     4381 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc cgctcatgcg atgataagct
     4441 gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa
     4501 gagataaatt gcactgaaat ctagaaatat tttatctgat taataagatg atcttcttga
     4561 gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc
     4621 ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga
     4681 gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact
     4741 aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg
     4801 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     4861 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     4921 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     4981 agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5041 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     5101 aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag
     5161 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
     5221 cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg
     5281 tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca
     5341 tagtaagcca gtatacactc cgctagcgct gatgtccggc ggtgcttttg ccgttacgca
     5401 ccaccccgtc atgagattac gcgttttagc ggtgcgtaca attaagggat tatggtaaat
     5461 ccacttactg tctgccctcg tagccatcga gataaaccgc agtactccgg ccacgatgcg
     5521 tccggcgtag aggatcgaga tct
//
