LOCUS       Exported                6508 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-31Kp-IRAK2      6508 bp    DNA   circular            
            8/29/2017

            DEFINITION  Current Name: pF-31Kp - IRAK2
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1577
                     /label=N terminal NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1594..3477
                     /label=IRAK2 insert
                     /note="This insert contains an Sgf I site at position 0 of 
                     the unmodified insert. This insert contains a Pme I site at
                     position 1884 of the unmodified insert."
     misc_feature    3474..3481
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3478..3611
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    3633..3854
                     /label=Sv40 late polyadenylation signal
     promoter        3953..4371
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(4026..4262)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      4269..4334
                     /label=Sv40 Min Ori
     promoter        4379..4445
                     /label=EM7 bacterial promoter
     CDS             4459..5253
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5317..5365
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggcctgctac atctatcagc
     1621 tgccaagctg ggtgctggac gacctgtgcc ggaacatgga cgccctgtcc gagtgggatt
     1681 ggatggagtt cgcctcttac gtgatcaccg acctgacaca gctgcggaag atcaagtcta
     1741 tggagagagt gcagggcgtg agcatcaccc gggagctgct gtggtggtgg ggcatgagac
     1801 aggccacagt gcagcagctg gtggatctgc tgtgcaggct ggagctgtat cgcgccgccc
     1861 agatcatcct gaattggaag cctgccccag agatcaggtg ccccatccct gcctttcccg
     1921 acagcgtgaa gcctgagaag ccactggcag catccgtgag gaaggcagag gatgagcagg
     1981 aggagggaca gcctgtgcgc atggccacct tcccaggacc aggcagctcc ccagcacggg
     2041 cccaccagcc tgcctttctg cagccacctg aggaggacgc accacactct ctgcgcagcg
     2101 atctgcccac ctctagcgac tctaaggatt tcagcacatc catccctaag caggagaagc
     2161 tgctgagcct ggccggcgac tccctgtttt ggtctgaggc cgatgtggtg caggccaccg
     2221 acgatttcaa ccagaatagg aagatctccc agggcacatt tgccgacgtg tacaggggac
     2281 acagacacgg caagcccttc gtgtttaaga agctgaggga gaccgcatgc tcctctcctg
     2341 gaagcatcga gaggttcttt caggccgagc tgcagatctg cctgcgctgc tgtcacccaa
     2401 acgtgctgcc cgtgctggga ttctgtgcag caagacagtt ccactctttt atctacccct
     2461 atatggccaa tggcagcctg caggacagac tgcagggaca gggaggatcc gatcctctgc
     2521 catggcccca gagggtgtcc atctgctctg gcctgctgtg cgccgtggag tatctgcacg
     2581 gcctggagat catccacagc aacgtgaaga gcagcaatgt gctgctggat cagaacctga
     2641 cccctaagct ggcacaccca atggcacacc tgtgccctgt gaataagcgg agcaagtaca
     2701 ccatgatgaa gacacacctg ctgagaacct ccgccgccta tctgccagag gacttcatca
     2761 gggtgggcca gctgacaaag cgcgtggata tcttttcctg cggcatcgtg ctggccgagg
     2821 tgctgacagg catccctgcc atggacaaca atcgctcccc agtgtacctg aaggacctgc
     2881 tgctgtctga tatcccatct agcaccgcaa gcctgtgctc caggaagaca ggagtggaga
     2941 acgtgatggc caaggagatc tgtcagaagt atctggagaa gggagcaggc agactgccag
     3001 aggattgcgc agaggccctg gcaaccgcag catgcctgtg cctgcggaga aggaatacaa
     3061 gcctgcagga ggtgtgcgga tccgtggcag cagtggagga gcgcctgcgg ggcagagaga
     3121 ccctgctgcc atggtctggc ctgagcgagg gcacaggctc ctctagcaac acccccgagg
     3181 agacagacga tgtggacaat tcctctctgg atgccagctc ctctatgagc gtggcacctt
     3241 gggcaggagc agcaaccccc ctgctgccta cagagaacgg cgagggcagg ctgcgcgtga
     3301 tcgtgggaag ggaggcagac agctcctctg aggcatgcgt gggactggag ccaccacagg
     3361 atgtgaccga gacatcctgg cagatcgaga tcaacgaggc caagagaaag ctgatggaga
     3421 atatcctgct gtacaaggag gagaaggtgg actctatcga gctgtttggc ccagtttaaa
     3481 cgaattcggg ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat
     3541 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     3601 ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     3661 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3721 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3781 tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct
     3841 ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     3901 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     3961 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     4021 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4141 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4201 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4261 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4321 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taattaactg
     4381 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     4441 aaacccagga ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4501 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4561 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4621 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4681 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4741 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4801 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4861 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4921 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4981 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5041 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5101 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5161 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5221 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5281 accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta
     5341 catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatccgctc atgcgatgat
     5401 aagctgtcaa acatgagaat tacaacttat atcgtatggg gctgacttca ggtgctacat
     5461 ttgaagagat aaattgcact gaaatctaga aatattttat ctgattaata agatgatctt
     5521 cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc
     5581 agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg
     5641 gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca
     5701 agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt
     5761 tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg
     5821 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt
     5881 ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     5941 aggagagcgc acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6001 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     6061 atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct
     6121 tccaggaaat ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag
     6181 cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc
     6241 accggtgcag ccttttttct cctgccacat gaagcacttc actgacaccc tcatcagtgc
     6301 caacatagta agccagtata cactccgcta gcgctgatgt ccggcggtgc ttttgccgtt
     6361 acgcaccacc ccgtcatgag attacgcgtt ttagcggtgc gtacaattaa gggattatgg
     6421 taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagtac tccggccacg
     6481 atgcgtccgg cgtagaggat cgagatct
//
