LOCUS       Exported                7016 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nn5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nn5K3
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1065..1577
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1578..1589
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..3971
                     /label=DGKg Uniprot P49619-1 NM_001346.2
                     /note="DGKg Uniprot P49619-1 NM_001346.2"
     misc_feature    3972..3979
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    4131..4352
                     /label=Sv40 late polyadenylation signal
     promoter        4451..4869
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4877..4943
                     /label=EM7 bacterial promoter
     CDS             4957..5751
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5825..5873
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    5916..6838
                     /label=P15 ori
                     /note="P15 ori"
     misc_feature    6093
                     /label=C2129
                     /note="C2129"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gggtgaagaa cggtgggtct
     1621 ccctcactcc agaagaattt gaccaactcc agaaatattc agaatattcc tccaagaaga
     1681 taaaagatgc cttgactgaa tttaatgagg gtgggagcct caaacaatat gacccacatg
     1741 agccgattag ctatgatgtc ttcaagctgt tcatgagggc gtacctggag gtggaccttc
     1801 cccagccact gagcactcac ctcttcctgg ccttcagcca gaagcccaga cacgagacct
     1861 ctgaccaccc gacggaggga gccagcaaca gtgaggccaa cagcgcagat actaatatac
     1921 agaatgcaga taatgccacc aaagcagacg aggcctgtgc ccctgatact gaatcaaata
     1981 tggctgagaa gcaagcacca gctgaagacc aagtggctgc gacccccctg gaaccccccg
     2041 tccctcggtc ttcaagctcg gaatccccag tggtatacct gaaggatgtt gtgtgctacc
     2101 tgtccctgct ggagacgggg aggcctcagg ataagctgga gttcatgttt cgcctctatg
     2161 attcagatga gaacggtctc ctggaccaag cggagatgga ttgcattgtc aaccaaatgc
     2221 tgcatattgc ccagtacctg gagtgggatc ccacagagct gaggcctata ttgaaggaga
     2281 tgctgcaagg gatggactac gaccgggacg gctttgtgtc tctacaggaa tgggtccatg
     2341 gagggatgac caccatccca ttgctggtcc tcctggggat ggatgactct ggctccaagg
     2401 gggatgggcg gcacgcctgg accatgaagc acttcaagaa accaacctac tgcaacttct
     2461 gccatatcat gctcatgggc gtccgcaagc aaggcctgtg ctgcacttac tgtaaataca
     2521 ctgtccacga acgctgtgtg tccagaaaca ttcctggttg tgtcaaaacg tactcaaaag
     2581 ccaaaaggag tggtgaggtg atgcagcacg catgggtgga agggaactcc tccgtcaagt
     2641 gtgaccggtg ccacaaaagt atcaagtgct accagagtgt caccgcgcgg cactgcgtgt
     2701 ggtgccggat gacgtttcac cgcaaatgtg aattatcaac gttgtgtgac ggtggggaac
     2761 tcagagacca catcttactg cccacctcca tatgccccat cacccgggac aggccaggtg
     2821 agaagtctga tggctgcgtg tccgccaagg gcgaacttgt catgcagtat aagatcatcc
     2881 ccaccccggg tacccacccc ctgctggtct tggtgaaccc caagagtgga gggagacaag
     2941 gagaaagaat tcttcggaaa ttccactatc tgctcaaccc caaacaagtt ttcaacctgg
     3001 acaatggggg gcctactcca gggttgaact ttttccgtga tactccagac ttccgtgttt
     3061 tggcctgtgg tggagatggg acagttggct ggattttgga ttgcattgat aaggccaact
     3121 ttgcaaagca tccaccagtg gctgtcctgc ctcttggaac aggaaatgac cttgcccgtt
     3181 gtctccgctg gggaggaggt tatgaagggg gcagcttgac aaaaatcctg aaagacattg
     3241 agcagagccc cttggtgatg ctggaccgct ggcatctgga agtcatcccc agagaggaag
     3301 tggaaaacgg ggaccaggtc ccatacagca tcatgaacaa ctatttctcc attggtgtgg
     3361 acgcttccat tgcacacaga ttccatgtga tgagagagaa acatcctgaa aaattcaaca
     3421 gcaggatgaa gaacaagctg tggtactttg aatttggcac ctcggagact tttgcagcga
     3481 cctgcaagaa actccacgac cacattgagt tggagtgtga tggggttggg gtggacctga
     3541 gcaacatctt cctggaaggc attgccattc tcaacattcc cagcatgtac ggaggcacca
     3601 atctctgggg agaaaacaag aagaaccggg ctgtgatccg ggaaagcagg aagggtgtca
     3661 ctgaccccaa agaactgaaa ttctgcgttc aagacctcag tgaccagctc cttgaagtgg
     3721 tggggctaga aggagccatg gagatggggc agatctacac cggcctgaag agtgcaggca
     3781 ggaggctggc ccagtgcgcc tctgtcacca tcaggacaaa caagctgctg ccaatgcaag
     3841 tggatggaga accctggatg cagccatgtt gcacgattaa aattactcac aagaaccaag
     3901 cgcccatgat gatggggcct ccccagaaga gcagcttctt ctcgttgaga aggaagagcc
     3961 gttcaaaaga cgtttaaacg aattcgggct cggtacccgg ggatcctcta gagtcgacct
     4021 gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     4081 gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat
     4141 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4201 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4261 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4321 tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc
     4381 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4441 acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
     4501 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4561 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4681 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4801 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4861 aaaaagctta attaactgtt gacaattaat catcggcata gtatatcggc atagtataat
     4921 acgacaaggt gaggaactaa acccaggagg cagatcatga ttgaacaaga tggattgcac
     4981 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5041 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5101 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5161 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5221 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5281 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5341 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5401 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5461 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5521 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5581 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5641 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5701 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5761 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg ccgdgkgtnr
     5821 tnmcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     5881 agcgataagg atccgctcat gcgatgataa gctgtcaaac atgagaatta caacttatat
     5941 cgtatggggc tgacttcagg tgctacattt gaagagataa attgcactga aatctagaaa
     6001 tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct
     6061 tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc
     6121 aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca
     6181 gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc
     6241 tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga
     6301 taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     6361 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa
     6421 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa
     6481 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt
     6541 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac
     6601 ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat
     6661 ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     6721 tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga
     6781 agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc
     6841 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcatgagat tacgcgtttt
     6901 agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat
     6961 cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg agatct
//
