LOCUS       Exported                6116 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-32Kp-TGFBR1      6116 bp    DNA   circular           
            5/31/2016

            DEFINITION  Current Name: pF-32Kp - TGFBR1
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1060..2577
                     /label=TGFBR1 insert
     misc_feature    2574..2580
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    2580..2594
                     /label=C Terminal NanoLuc linker
     misc_feature    2583..2594
                     /label=N Terminal NanoLuc linker
     CDS             2595..3104
                     /label=C terminal NanoLuc
     polyA_signal    3241..3462
                     /label=Sv40 late polyadenylation signal
     promoter        3561..3979
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        3987..4053
                     /label=EM7 bacterial promoter
     CDS             4067..4861
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4925..4973
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggag gcggcggtcg
     1081 ctgctccgcg tccccggctg ctcctcctcg tgctggcggc ggcggcggcg gcggcggcgg
     1141 cgctgctccc gggggcgacg gcgttacagt gtttctgcca cctctgtaca aaagacaatt
     1201 ttacttgtgt gacagatggg ctctgctttg tctctgtcac agagaccaca gacaaagtta
     1261 tacacaacag catgtgtata gctgaaattg acttaattcc tcgagatagg ccgtttgtat
     1321 gtgcaccctc ttcaaaaact gggtctgtga ctacaacata ttgctgcaat caggaccatt
     1381 gcaataaaat agaacttcca actactgtaa agtcatcacc tggccttggt cctgtggaac
     1441 tggcagctgt cattgctgga ccagtgtgct tcgtctgcat ctcactcatg ttgatggtct
     1501 atatctgcca caaccgcact gtcattcacc atcgagtgcc aaatgaagag gacccttcat
     1561 tagatcgccc ttttatttca gagggtacta cgttgaaaga cttaatttat gatatgacaa
     1621 cgtcaggttc tggctcaggt ttaccattgc ttgttcagag aacaattgcg agaactattg
     1681 tgttacaaga aagcattggc aaaggtcgat ttggagaagt ttggagagga aagtggcggg
     1741 gagaagaagt tgctgttaag atattctcct ctagagaaga acgttcgtgg ttccgtgagg
     1801 cagagattta tcaaactgta atgttacgtc atgaaaacat cctgggattt atagcagcag
     1861 acaataaaga caatggtact tggactcagc tctggttggt gtcagattat catgagcatg
     1921 gatccctttt tgattactta aacagataca cagttactgt ggaaggaatg ataaaacttg
     1981 ctctgtccac ggcgagcggt cttgcccatc ttcacatgga gattgttggt acccaaggaa
     2041 agccagccat tgctcataga gatttgaaat caaagaatat cttggtaaag aagaatggaa
     2101 cttgctgtat tgcagactta ggactggcag taagacatga ttcagccaca gataccattg
     2161 atattgctcc aaaccacaga gtgggaacaa aaaggtacat ggcccctgaa gttctcgatg
     2221 attccataaa tatgaaacat tttgaatcct tcaaacgtgc tgacatctat gcaatgggct
     2281 tagtattctg ggaaattgct cgacgatgtt ccattggtgg aattcatgaa gattaccaac
     2341 tgccttatta tgatcttgta ccttctgacc catcagttga agaaatgaga aaagttgttt
     2401 gtgaacagaa gttaaggcca aatatcccaa acagatggca gagctgtgaa gccttgagag
     2461 taatggctaa aattatgaga gaatgttggt atgccaatgg agcagctagg cttacagcat
     2521 tgcggattaa gaaaacatta tcgcaactca gtcaacagga aggcatcaaa atggtttctc
     2581 tcggctcgag cggcgtcttc acactcgaag atttcgttgg ggactggcga cagacagccg
     2641 gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg
     2701 gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg
     2761 acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa
     2821 aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg
     2881 gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg
     2941 aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca
     3001 acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca
     3061 tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcgtaaggc cgcgactcta
     3121 gagtcgacct gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga
     3181 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag
     3241 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3301 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3361 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     3421 gggaggtttt tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa
     3481 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3541 cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     3601 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3721 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3781 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3841 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3901 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3961 aggcttttgc aaaaagctta attaactgtt gacaattaat catcggcata gtatatcggc
     4021 atagtataat acgacaaggt gaggaactaa acccaggagg cagatcatga ttgaacaaga
     4081 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4141 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4201 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     4261 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4321 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4381 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4441 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4501 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4561 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     4621 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4681 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4741 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4801 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4861 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg
     4921 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     4981 agcgataagg atccgctcat gcgatgataa gctgtcaaac atgagaatta caacttatat
     5041 cgtatggggc tgacttcagg tgctacattt gaagagataa attgcactga aatctagaaa
     5101 tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct
     5161 tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc
     5221 aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca
     5281 gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc
     5341 tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga
     5401 taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     5461 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa
     5521 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa
     5581 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt
     5641 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac
     5701 ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat
     5761 ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     5821 tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga
     5881 agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc
     5941 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcatgagat tacgcgtttt
     6001 agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat
     6061 cgagataaac cgcagtactc cggccacgat gcgtccggcg tagaggatcg agatct
//
