LOCUS       Exported                6487 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-31Kp-MAP2K5      5977 bp    DNA   circular           
            5/31/2016

            DEFINITION  Current Name: pF-31Kp - MAP2K5
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1577
                     /label=N terminal NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..3452
                     /label=cIAP1
                     /note="BIRC2"
     misc_feature    1736..1936
                     /label=BIR1
                     /note="BIR1"
     misc_feature    2150..2347
                     /label=BIR2
                     /note="BIR2"
     misc_feature    2405..2605
                     /label=BIR3
                     /note="BIR3"
     misc_feature    2957..3226
                     /label=DOMAIN
     misc_feature    3455..3460
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3461..3590
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    3612..3833
                     /label=Sv40 late polyadenylation signal
     promoter        3932..4350
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4358..4424
                     /label=EM7 bacterial promoter
     CDS             4438..5232
                     /label=Neo R / Kan R
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5296..5344
                     /label=Synthetic PolyAdenylation Signal
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gcacaaaact gcctcccaaa
     1621 gacttttccc aggtccctcg tatcaaaaca ttaagagtat aatggaagat agcacgatct
     1681 tgtcagattg gacaaacagc aacaaacaaa aaatgaagta tgacttttcc tgtgaactct
     1741 acagaatgtc tacatattca actttccccg ccggggtgcc tgtctcagaa aggagtcttg
     1801 ctcgtgctgg tttctattat actggtgtga atgacaaggt caaatgcttc tgttgtggcc
     1861 tgatgctgga taactggaaa ctaggagaca gtcctattca aaagcataaa cagctatatc
     1921 ctagctgtag ctttattcag aatctggttt cagctagtct gggatccacc tctaagaata
     1981 cgtctccaat gagaaacagt tttgcacatt cattatctcc caccttggaa catagtagct
     2041 tgttcagtgg ttcttactcc agcctttctc caaaccctct taattctaga gcagttgaag
     2101 acatctcttc atcgaggact aacccctaca gttatgcaat gagtactgaa gaagccagat
     2161 ttcttaccta ccatatgtgg ccattaactt ttttgtcacc atcagaattg gcaagagctg
     2221 gtttttatta tataggacct ggagataggg tagcctgctt tgcctgtggt gggaagctca
     2281 gtaactggga accaaaggat gatgctatgt cagaacaccg gaggcatttt cccaactgtc
     2341 catttttgga aaattctcta gaaactctga ggtttagcat ttcaaatctg agcatgcaga
     2401 cacatgcagc tcgaatgaga acatttatgt actggccatc tagtgttcca gttcagcctg
     2461 agcagcttgc aagtgctggc ttttattatg tgggtcgcaa tgatgatgtc aaatgctttt
     2521 gttgtgatgg tggcttgagg tgttgggaat ctggagatga tccatgggta gaacatgcca
     2581 agtggtttcc aaggtgtgag ttcttgatac gaatgaaagg ccaagagttt gttgatgaga
     2641 ttcaaggtag atatcctcat cttcttgaac agctgttgtc aacttcagat accactggag
     2701 aagaaaatgc tgacccacca attattcatt ttggacctgg agaaagttct tcagaagatg
     2761 ctgtcatgat gaatacacct gtggttaaat ctgccttgga aatgggcttt aatagagacc
     2821 tggtgaaaca aacagttcaa agtaaaatcc tgacaactgg agagaactat aaaacagtta
     2881 atgatattgt gtcagcactt cttaatgctg aagatgaaaa aagagaagag gagaaggaaa
     2941 aacaagctga agaaatggca tcagatgatt tgtcattaat tcggaagaac agaatggctc
     3001 tctttcaaca attgacatgt gtgcttccta tcctggataa tcttttaaag gccaatgtaa
     3061 ttaataaaca ggaacatgat attattaaac aaaaaacaca gataccttta caagcgagag
     3121 aactgattga taccattttg gttaaaggaa atgctgcggc caacatcttc aaaaactgtc
     3181 taaaagaaat tgactctaca ttgtataaga acttatttgt ggataagaat atgaagtata
     3241 ttccaacaga agatgtttca ggtctgtcac tggaagaaca attgaggagg ttgcaagaag
     3301 aacgaacttg taaagtgtgt atggacaaag aagtttctgt tgtatttatt ccttgtggtc
     3361 atctggtagt atgccaggaa tgtgcccctt ctctaagaaa atgccctatt tgcaggggta
     3421 taatcaaggg tactgttcgt acatttctct ctgtttaaac gaattcgggc tcggtacccg
     3481 gggatcctct agagtcgacc tgcaggcatg caagctgatc cggctgctaa caaagcccga
     3541 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcg
     3601 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3661 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3721 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3781 gggggagatg tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     3841 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     3901 gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct
     3961 gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt
     4021 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4081 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4141 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4201 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4261 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4321 tttggaggcc taggcttttg caaaaagctt aattaactgt tgacaattaa tcatcggcat
     4381 agtatatcgg catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg
     4441 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4501 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4561 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     4621 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4681 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4741 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4801 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4861 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4921 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     4981 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5041 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5101 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5161 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5221 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5281 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     5341 tgtgaatcga tagcgataag gatccgctca tgcgatgata agctgtcaaa catgagaatt
     5401 acaacttata tcgtatgggg ctgacttcag gtgctacatt tgaagagata aattgcactg
     5461 aaatctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc
     5521 gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc
     5581 tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac
     5641 ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa
     5701 ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga
     5761 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     5821 ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
     5881 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     5941 gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     6001 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc
     6061 ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt
     6121 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
     6181 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc
     6241 ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac
     6301 actccgctag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcatgaga
     6361 ttacgcgttt tagcggtgcg tacaattaag ggattatggt aaatccactt actgtctgcc
     6421 ctcgtagcca tcgagataaa ccgcagtact ccggccacga tgcgtccggc gtagaggatc
     6481 gagatct
//
