LOCUS       Exported                8844 bp ds-DNA     circular SYN 26-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 26, 2021 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
COMMENT     LOCUS       UnnamedSequence      5152 bp    DNA   circular          
             9/13/2011

            DEFINITION  Current Name: Unnamed Sequence
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from %22CMV 
                     Promoter%22 to %22CMV immediate-early enhancer/promoter%22 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     misc_feature    1056..1060
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1061..5136
                     /label=Untitled
     misc_feature    1061..5136
                     /label=KDR(V1) insert
                     /note="Homo sapiens kinase insert domain receptor (a type 
                     III receptor tyrosine kinase) (KDR), mRNA
                     This insert was automatically updated on 2/14/2007. Any 
                     NCBI Synonyms, Name, Description and the comparison to the 
                     accession number were all automatically updated from NCBI."
     misc_feature    1061..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1143..1149
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    3703..3708
                     /label=EcoICR I
     misc_difference 5133..5135
                     /label=extra GTT tracked back to input pF1K
                     /note="extra GTT tracked back to input pF1K KDR plasmid"
     misc_feature    5137..5139
                     /label=EcoICR I
     stem_loop       5139..5186
                     /label=linker
                     /note="linker"
     gene            5187..5696
                     /label=Nluc
                     /note="missing starting Met"
     polyA_signal    5833..6054
                     /label=Sv40 late polyadenylation signal
     promoter        6153..6571
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm %22SV40 early enhancer/promoter 
                     region%22 to %22SV40 enhancer and early promoter%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(6226..6462)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      6469..6534
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        6579..6645
                     /label=EM7 bacterial promoter
     CDS             6659..7453
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm %22Neomycin phosphotransferase%22 
                     to %22Neomycin phosphotransferase coding region%22 on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     polyA_signal    7517..7565
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      7801..7837
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    8508..8793
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcag agcaaggtgc
     1081 tgctggccgt cgccctgtgg ctctgcgtgg agacccgggc cgcctctgtg ggtttgccta
     1141 gtgtttctct tgatctgccc aggctcagca tacaaaaaga catacttaca attaaggcta
     1201 atacaactct tcaaattact tgcaggggac agagggactt ggactggctt tggcccaata
     1261 atcagagtgg cagtgagcaa agggtggagg tgactgagtg cagcgatggc ctcttctgta
     1321 agacactcac aattccaaaa gtgatcggaa atgacactgg agcctacaag tgcttctacc
     1381 gggaaactga cttggcctcg gtcatttatg tctatgttca agattacaga tctccattta
     1441 ttgcttctgt tagtgaccaa catggagtcg tgtacattac tgagaacaaa aacaaaactg
     1501 tggtgattcc atgtctcggg tccatttcaa atctcaacgt gtcactttgt gcaagatacc
     1561 cagaaaagag atttgttcct gatggtaaca gaatttcctg ggacagcaag aagggcttta
     1621 ctattcccag ctacatgatc agctatgctg gcatggtctt ctgtgaagca aaaattaatg
     1681 atgaaagtta ccagtctatt atgtacatag ttgtcgttgt agggtatagg atttatgatg
     1741 tggttctgag tccgtctcat ggaattgaac tatctgttgg agaaaagctt gtcttaaatt
     1801 gtacagcaag aactgaacta aatgtgggga ttgacttcaa ctgggaatac ccttcttcga
     1861 agcatcagca taagaaactt gtaaaccgag acctaaaaac ccagtctggg agtgagatga
     1921 agaaattttt gagcacctta actatagatg gtgtaacccg gagtgaccaa ggattgtaca
     1981 cctgtgcagc atccagtggg ctgatgacca agaagaacag cacatttgtc agggtccatg
     2041 aaaaaccttt tgttgctttt ggaagtggca tggaatctct ggtggaagcc acggtggggg
     2101 agcgtgtcag aatccctgcg aagtaccttg gttacccacc cccagaaata aaatggtata
     2161 aaaatggaat accccttgag tccaatcaca caattaaagc ggggcatgta ctgacgatta
     2221 tggaagtgag tgaaagagac acaggaaatt acactgtcat ccttaccaat cccatttcaa
     2281 aggagaagca gagccatgtg gtctctctgg ttgtgtatgt cccaccccag attggtgaga
     2341 aatctctaat ctctcctgtg gattcctacc agtacggcac cactcaaacg ctgacatgta
     2401 cggtctatgc cattcctccc ccgcatcaca tccactggta ttggcagttg gaggaagagt
     2461 gcgccaacga gcccagccaa gctgtctcag tgacaaaccc atacccttgt gaagaatgga
     2521 gaagtgtgga ggacttccag ggaggaaata aaattgaagt taataaaaat caatttgctc
     2581 taattgaagg aaaaaacaaa actgtaagta cccttgttat ccaagcggca aatgtgtcag
     2641 ctttgtacaa atgtgaagcg gtcaacaaag tcgggagagg agagagggtg atctccttcc
     2701 acgtgaccag gggtcctgaa attactttgc aacctgacat gcagcccact gagcaggaga
     2761 gcgtgtcttt gtggtgcact gcagacagat ctacgtttga gaacctcaca tggtacaagc
     2821 ttggcccaca gcctctgcca atccatgtgg gagagttgcc cacacctgtt tgcaagaact
     2881 tggatactct ttggaaattg aatgccacca tgttctctaa tagcacaaat gacattttga
     2941 tcatggagct taagaatgca tccttgcagg accaaggaga ctatgtctgc cttgctcaag
     3001 acaggaagac caagaaaaga cattgcgtgg tcaggcagct cacagtccta gagcgtgtgg
     3061 cacccacgat cacaggaaac ctggagaatc agacgacaag tattggggaa agcatcgaag
     3121 tctcatgcac ggcatctggg aatccccctc cacagatcat gtggtttaaa gataatgaga
     3181 cccttgtaga agactcaggc attgtattga aggatgggaa ccggaacctc actatccgca
     3241 gagtgaggaa ggaggacgaa ggcctctaca cctgccaggc atgcagtgtt cttggctgtg
     3301 caaaagtgga ggcatttttc ataatagaag gtgcccagga aaagacgaac ttggaaatca
     3361 ttattctagt aggcacggcg gtgattgcca tgttcttctg gctacttctt gtcatcatcc
     3421 tacggaccgt taagcgggcc aatggagggg aactgaagac aggctacttg tccatcgtca
     3481 tggatccaga tgaactccca ttggatgaac attgtgaacg actgccttat gatgccagca
     3541 aatgggaatt ccccagagac cggctgaagc taggtaagcc tcttggccgt ggtgcctttg
     3601 gccaagtgat tgaagcagat gcctttggaa ttgacaagac agcaacttgc aggacagtag
     3661 cagtcaaaat gttgaaagaa ggagcaacac acagtgagca tcgagctctc atgtctgaac
     3721 tcaagatcct cattcatatt ggtcaccatc tcaatgtggt caaccttcta ggtgcctgta
     3781 ccaagccagg agggccactc atggtgattg tggaattctg caaatttgga aacctgtcca
     3841 cttacctgag gagcaagaga aatgaatttg tcccctacaa gaccaaaggg gcacgattcc
     3901 gtcaagggaa agactacgtt ggagcaatcc ctgtggatct gaaacggcgc ttggacagca
     3961 tcaccagtag ccagagctca gccagctctg gatttgtgga ggagaagtcc ctcagtgatg
     4021 tagaagaaga ggaagctcct gaagatctgt ataaggactt cctgaccttg gagcatctca
     4081 tctgttacag cttccaagtg gctaagggca tggagttctt ggcatcgcga aagtgtatcc
     4141 acagggacct ggcggcacga aatatcctct tatcggagaa gaacgtggtt aaaatctgtg
     4201 actttggctt ggcccgggat atttataaag atccagatta tgtcagaaaa ggagatgctc
     4261 gcctcccttt gaaatggatg gccccagaaa caatttttga cagagtgtac acaatccaga
     4321 gtgacgtctg gtcttttggt gttttgctgt gggaaatatt ttccttaggt gcttctccat
     4381 atcctggggt aaagattgat gaagaatttt gtaggcgatt gaaagaagga actagaatga
     4441 gggcccctga ttatactaca ccagaaatgt accagaccat gctggactgc tggcacgggg
     4501 agcccagtca gagacccacg ttttcagagt tggtggaaca tttgggaaat ctcttgcaag
     4561 ctaatgctca gcaggatggc aaagactaca ttgttcttcc gatatcagag actttgagca
     4621 tggaagagga ttctggactc tctctgccta cctcacctgt ttcctgtatg gaggaggagg
     4681 aagtatgtga ccccaaattc cattatgaca acacagcagg aatcagtcag tatctgcaga
     4741 acagtaagcg aaagagccgg cctgtgagtg taaaaacatt tgaagatatc ccgttagaag
     4801 aaccagaagt aaaagtaatc ccagatgaca accagacgga cagtggtatg gttcttgcct
     4861 cagaagagct gaaaactttg gaagacagaa ccaaattatc tccatctttt ggtggaatgg
     4921 tgcccagcaa aagcagggag tctgtggcat ctgaaggctc aaaccagaca agcggctacc
     4981 agtccggata tcactccgat gacacagaca ccaccgtgta ctccagtgag gaagcagaac
     5041 ttttaaagct gatagagatt ggagtgcaaa ccggtagcac agcccagatt ctccagcctg
     5101 actcggggac cacactgagc tctcctcctg ttgtttctct cggtggttca ggtggtggcg
     5161 ggagcggtgg agggagcagc ggtggagtct tcacactcga agatttcgtt ggggactggc
     5221 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     5281 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     5341 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     5401 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     5461 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     5521 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     5581 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     5641 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag
     5701 gccgcgactc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg
     5761 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     5821 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     5881 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     5941 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     6001 agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg
     6061 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     6121 tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc
     6181 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     6241 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6301 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6361 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6421 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6481 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6541 ttttggaggc ctaggctttt gcaaaaagct taattaactg ttgacaatta atcatcggca
     6601 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
     6661 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6721 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6781 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6841 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6901 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6961 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7021 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7081 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7141 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     7201 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7261 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7321 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7381 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7441 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     7501 ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     7561 gtgtgaatcg atagcgataa ggatcctctt tgcgcttgcg ttttcccttg tccagatagc
     7621 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacccggt
     7681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8221 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc
     8401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaaatacagg
     8521 aacgcacgct ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt
     8581 ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc ataggtattc atacggttaa
     8641 aatttatcag gcgcgattgc ggcagttttt cgggtggttt gttgccattt ttacctgtct
     8701 gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac aattaaggga ttatggtaaa
     8761 tccacttact gtctgccctc gtagccatcg agataaaccg cagtactccg gccacgatgc
     8821 gtccggcgta gaggatcgag atct
//
