LOCUS       pET-hIRF5-V5(4D)        6784 bp DNA     circular SYN 19-MAR-2025
DEFINITION  Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2025 from SnapGene 7.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
FEATURES             Location/Qualifiers
     source          1..6784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1613..1711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1661..1681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1682..1711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    71..1612
                     /label=human IRF V5
     misc_feature    1421..1423
                     /label=S/D Mut1
     misc_feature    1427..1429
                     /label=S/D Mut2
     misc_feature    1436..1438
                     /label=S/D Mut3
     misc_feature    1454..1456
                     /label=S/D Mut4
     misc_feature    1613..1660
                     /label=Classic Furin cleavable linker flanked by GGS
     misc_feature    1661..1681
                     /label=Sortag
                     /note="/created_by=jessiedavis"
     misc_feature    1681
                     /label=junction 1
                     /label=nonstandard type: Junction
                     /note="/Deletion_Size=30"
                     /note="/ID=junction 1"
                     /note="/Junction_Destination=1229+"
                     /note="/Junction_Source=1198+"
                     /note="/Junction_Type=Deletion"
                     /note="/Reads_supporting_discovery=4"
                     /note="/Reads_using=4"
     CDS             1694..1711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    1712
                     /label=junction 1
                     /label=nonstandard type: Junction
                     /note="/Deletion_Size=30"
                     /note="/ID=junction 1"
                     /note="/Junction_Destination=1229+"
                     /note="/Junction_Source=1198+"
                     /note="/Junction_Type=Deletion"
                     /note="/Reads_supporting_discovery=4"
                     /note="/Reads_using=4"
     CDS             1730..1774
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(1788..1810)
                     /label=HR166
                     /note="/%GC=47.8"
                     /note="/Sequence=GGCAGCAGCCTAGGTTAATTAAG"
                     /note="/Transferred_From=HR166"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(1812..1830)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1826..1873
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1910..2365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1936..1968
                     /label=NMG-306
                     /note="/%GC=72.7"
                     /note="/Sequence=GCGGCGGGTGTGGTGGTTACGCGCAGCGTGACC"
                     /note="/Transferred_From=NMG-306"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(1997..2016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2207..2228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2472..2500
                     /label=NMG-853
                     /note="/%GC=37.9"
                     /note="/Sequence=TCTGACAGTTACCAATGCTTAATCAGTGA"
                     /note="/Transferred_From=NMG-853"
                     /note="/Transferred_Similarity=100.00%"
     CDS             complement(2480..3340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2630..2659
                     /label=NMG-584
                     /note="/%GC=43.3"
                     /note="/Sequence=CTCACCGGCTCCAGATTTATCAGCAATAAA"
                     /note="/Transferred_From=NMG-584"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     2721..2745
                     /label=ACK-12
                     /note="/%GC=40.0"
                     /note="/Sequence=TCTATTAATTGTTGCCGGGAAGCTA"
                     /note="/Transferred_From=ACK-12"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     2829..2855
                     /label=NMG-1102
                     /note="/%GC=51.9"
                     /note="/Sequence=ATGGCTTCATTCAGCTCCGGTTCCCAA"
                     /note="/Transferred_From=NMG-1102"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     3004..3032
                     /label=NMG-854
                     /note="/%GC=48.3"
                     /note="/Sequence=CCGTAAGATGCTTTTCTGTGACTGGTGAG"
                     /note="/Transferred_From=NMG-854"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     3103..3122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     3164..3193
                     /label=NMG-585
                     /note="/%GC=46.7"
                     /note="/Sequence=GCGAAAACTCTCAAGGATCTTACCGCTGTT"
                     /note="/Transferred_From=NMG-585"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(3207..3234)
                     /label=JRD750
                     /note="/%GC=50.0"
                     /note="/Hairpin_Tm=None"
                     /note="/Self_Dimer_Tm=11.5"
                     /note="/Sequence=ATCAGTTGGGTGCACGAGTGGGTTACAT"
                     /note="/Tm=68.1"
                     /note="/created_by=primer3"
                     /note="/modified_by=jessiedavis"
     primer_bind     3221..3245
                     /label=ACK-13
                     /note="/%GC=48.0"
                     /note="/Sequence=TGCACCCAACTGATCTTCAGCATCT"
                     /note="/Transferred_From=ACK-13"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     3224..3254
                     /label=JRD749
                     /note="/%GC=38.7"
                     /note="/Hairpin_Tm=48.1"
                     /note="/Self_Dimer_Tm=4.5"
                     /note="/Sequence=ACCCAACTGATCTTCAGCATCTTTTACTTTC"
                     /note="/Tm=63.9"
                     /note="/created_by=primer3"
     misc_feature    3224..3234
                     /label=nonstandard type: USER
                     /note="/created_by=jessiedavis"
     primer_bind     3322..3351
                     /label=NMG-396
                     /note="/%GC=33.3"
                     /note="/Sequence=GGAAATGTTGAATACTCATACTCTTCCTTT"
                     /note="/Transferred_From=NMG-396"
                     /note="/Transferred_Similarity=100.00%"
     promoter        complement(3341..3433)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3380..3402
                     /label=NMG-1469
                     /note="/%GC=43.5"
                     /note="/Sequence=GTTATTGTCTCATGAGCGGATAC"
                     /note="/Transferred_From=NMG-1469"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     3396..3422
                     /label=NMG-1103
                     /note="/%GC=25.9"
                     /note="/Sequence=CGGATACATATTTGAATGTATTTAGAA"
                     /note="/Transferred_From=NMG-1103"
                     /note="/Transferred_Similarity=100.00%"
     rep_origin      3514..4102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3577..3601)
                     /label=ACK-11
                     /note="/%GC=52.0"
                     /note="/Sequence=CTCTTGATCCGGCAAACAAACCACC"
                     /note="/Transferred_From=ACK-11"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     3735..3754
                     /label=QB0767
                     /label=QB0767; RepO-R
                     /note="/%GC=55.0"
                     /note="/Sequence=TCCTGTTACCAGTGGCTGCT"
                     /note="/Transferred_From=QB0767, RepO-R"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     3778..3804
                     /label=NMG-309
                     /note="/%GC=48.1"
                     /note="/Sequence=TACCGGGTTGGACTCAAGACGATAGTT"
                     /note="/Transferred_From=NMG-309"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(3861..3879)
                     /label=QB0766
                     /label=QB0766; RepO-F
                     /note="/%GC=57.9"
                     /note="/Sequence=GTGTAGGTCGTTCGCTCCA"
                     /note="/Transferred_From=QB0766, RepO-F"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(3867..3894)
                     /label=NMG-851
                     /note="/%GC=50.0"
                     /note="/Sequence=AGGTATCTCAGTTCGGTGTAGGTCGTTC"
                     /note="/Transferred_From=NMG-851"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4003..4022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4078..4102)
                     /label=ACK-10
                     /note="/%GC=64.0"
                     /note="/Sequence=TTTCCATAGGCTCCGCCCCCCTGAC"
                     /note="/Transferred_From=ACK-10"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     4256..4273
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4288..4430
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4320..4352
                     /label=NMG-310
                     /note="/%GC=48.5"
                     /note="/Sequence=GCGGTATTTCACACCGCATATATGGTGCACTCT"
                     /note="/Transferred_From=NMG-310"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(4356..4375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     4516..4538
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4532..4723)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low
                     copy number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4856..4885
                     /label=NMG-311
                     /note="/%GC=63.3"
                     /note="/Sequence=GGCGGTATGGATGCGGCGGGACCAGAGAAA"
                     /note="/Transferred_From=NMG-311"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(5253..5272)
                     /label=Lac F
                     /label=Lac F; Lac-F
                     /note="/%GC=50.0"
                     /note="/Sequence=TAGCTCACTCATTAGGCACC"
                     /note="/Transferred_From=Lac F, Lac-F"
                     /note="/Transferred_Similarity=100.00%"
     CDS             complement(5295..6377)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5319..5345
                     /label=NMG-1101
                     /note="/%GC=48.1"
                     /note="/Sequence=AACCTGTCGTGCCAGCTGCATTAATGA"
                     /note="/Transferred_From=NMG-1101"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     5685..5712
                     /label=NMG-313
                     /note="/%GC=42.9"
                     /note="/Sequence=TTGCATGGTTTGTTGAAAACCGGACATG"
                     /note="/Transferred_From=NMG-313"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     6207..6236
                     /label=NMG-314
                     /note="/%GC=60.0"
                     /note="/Sequence=CGCCAGTTGTTGTGCCACGCGGTTGGGAAT"
                     /note="/Transferred_From=NMG-314"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     6339..6358
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6378..6455)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6495..6514
                     /label=JRDSeq2
                     /note="/%GC=60.0"
                     /note="/Sequence=GGAAGCAGCCCAGTAGTAGG"
                     /note="/Transferred_From=JRDSeq2"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     6661..6680
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     primer_bind     6707..6728
                     /label=HR129
                     /note="/%GC=68.2"
                     /note="/Sequence=GTGATGCCGGCCACGATGCGTC"
                     /note="/Transferred_From=HR129"
                     /note="/Transferred_Similarity=100.00%"
     promoter        join(6768..6784,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     join(6768..6784,1..2)
                     /note="/%GC=36.8"
                     /note="/Sequence=TAATACGACTCACTATAGG"
                     /note="/Transferred_From"
                     /note="/Transferred_Similarity=100.00%"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgaatcaat caatacccgt agctccaaca ccgccacgtc gtgttcgcct
      121 gaagccgtgg ctggtggcac aagtcaatag ctgccagtac ccgggtttgc aatgggttaa
      181 cggggagaag aagttgttct gcattccgtg gcgtcacgcg acccgtcatg gtccgagcca
      241 ggacggcgat aacaccattt ttaaagcctg ggcaaaagag acaggtaaat ataccgaagg
      301 tgttgatgaa gcggacccgg cgaagtggaa agccaatctg cgttgcgctc tgaacaaaag
      361 ccgtgatttt aggctaattt atgatggtcc gcgcgacatg ccgccgcagc catacaaaat
      421 ctatgaagta tgtagcaacg gcccggcgcc gacggacagc cagccgcctg aagactacag
      481 ctttggagcg ggcgaggagg aggaggagga ggaggaactt caacgcatgc taccgtccct
      541 gtccctgacc gacgctgtgc aatccggccc acacatgacc ccgtactctc tgctgaaaga
      601 agacgtgaag tggccaccga ccttgcaacc gcctacttta agacctccga cccttcaacc
      661 accgaccctg caaccgccgg ttgttttggg cccaccggct ccggacccgt cgccgctggc
      721 gccgccgcct ggtaatccgg cgggcttccg cgaactgttg agcgaagttt tggagccggg
      781 tccgctgccg gcttcgttgc cgccggcggg tgagcagctg ctacccgacc tactcatctc
      841 cccgcacatg ctcccgctga ctgacctgga gattaagttc cagtatcgcg gtcgtccgcc
      901 gcgtgcactg acgatctcca accctcacgg ctgccgtttg ttctacagtc aattggaggc
      961 tacccaggag caggtcgagc tgttcggtcc gatttccctg gagcaggtac gttttccgag
     1021 cccggaggac atcccaagcg ataagcagcg tttctatacg aaccagctcc tggatgtgct
     1081 ggatcgcggc ctgatcctgc aactgcaagg tcaagatctg tacgcaattc gtctttgcca
     1141 gtgtaaagtg ttctggtcag gcccgtgtgc gtctgcgcac gatagctgcc cgaaccccat
     1201 ccagagagaa gttaagacca aattgttcag cttggagcac tttctgaatg aactgatcct
     1261 gttccagaaa ggtcagacca atactccgcc accgtttgaa atcttctttt gcttcggcga
     1321 ggaatggcct gaccgcaaac cgcgtgaaaa gaagctgatc accgttcaag tggtgccggt
     1381 tgccgcacgt ctgttactgg agatgttttc tggtgagctg gattgggatg cggatgatat
     1441 tcgcttacaa atcgataacc cggacctgaa ggaccgtatg gtggaacagt ttaaggaact
     1501 gcatcatatt tggcaaagcc agcagcgtct gcagccggtc gctcaagcgc ctccgggcgc
     1561 gggcttaggt gtgggtcagg gtccgtggcc gatgcatccg gcgggcatgc aggggggaag
     1621 tggtggatca agagtacgaa ggggcggtag cggtggctcc ctcccagaga ccggtgtcgt
     1681 agacggcgga tcccatcacc accaccatca ctaatgactc gagtctggta aagaaaccgc
     1741 tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact agcgcagctt aattaaccta
     1801 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     1861 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
     1921 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1981 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2041 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2101 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2161 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2221 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2281 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     2341 aacaaaatat taacgtttac aatttctggc ggcacgatgg catgagatta tcaaaaagga
     2401 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2461 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2521 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2581 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2641 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2701 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2761 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2821 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2881 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2941 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3001 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3061 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3121 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3181 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3241 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3301 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatcat
     3361 gattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3421 aaaaataaac aaataggtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3481 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3541 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3601 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3661 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3901 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4021 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4081 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4141 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4201 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4261 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     4321 cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4381 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     4441 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4501 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4561 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     4621 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     4681 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     4741 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     4801 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     4861 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     4921 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     4981 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     5041 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     5101 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     5161 acgacaggag cacgatcatg ctagtcatgc cccgcgccca ccggaaggag ctgactgggt
     5221 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     5281 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5341 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     5401 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     5461 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     5521 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc
     5581 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     5641 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     5701 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     5761 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     5821 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     5881 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     5941 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     6001 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     6061 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     6121 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     6181 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     6241 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     6301 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     6361 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     6421 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     6481 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     6541 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     6601 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     6661 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     6721 gatgcgtccg gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
     6781 tata
//
