LOCUS       pSB-XPRESSO-hSpC       13113 bp DNA     circular SYN 20-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Gepstein Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..237
                     /label=5' ITR
     polyA_signal    324..548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     570..584
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    587..1175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             693..704
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(join(1206..1916,1917..1919))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(1920..1973)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(1989..2036)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(2037..6137)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=hSpCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2130..2132)
                     /label=AA1337
     CDS             complement(2136..2138)
                     /label=AA1335
     CDS             complement(2487..2489)
                     /label=AA1218
     CDS             complement(2736..2738)
                     /label=AA1135
     CDS             complement(6162..6182)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6286..7302
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(7303..7579)
                     /label=chicken beta-actin promoter
     enhancer        7581..7958
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7972..8400
                     /label=455BP_Core_UCOE
     misc_feature    8501..9109
                     /label=Synthetic RPBSA Promoter
     misc_feature    9125..9133
                     /label=Kozak Sequence
     CDS             9131..9832
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     CDS             9842..9898
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9899..10498
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_site      10663..10718
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     polyA_signal    10663..10718
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    complement(10868..11104)
                     /label=3' ITR
     rep_origin      complement(11281..11869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(12040..12831,12832..12900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12901..13005)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca
      301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
      361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
      421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
      481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
      541 aggcacagtc gaggctgatc agcgagctca ggtcgacggt atcgatgcgg ggaggcggcc
      601 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
      661 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
      721 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
      781 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
      841 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
      901 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
      961 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     1021 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     1081 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     1141 aatctttcac aaattttgta atccagaggt tgattatcga aagcttggcc tgacaggccg
     1201 tctcacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
     1261 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
     1321 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     1381 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
     1441 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
     1501 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     1561 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     1621 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     1681 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
     1741 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     1801 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     1861 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcact
     1921 gggccaggat tctcctcgac gtcaccgcat gttagcagac ttcctctgcc ctctccactg
     1981 ccgaattcct ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttgtcg
     2041 cctcccagct gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc
     2101 agggtggcgt ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca
     2161 aagtacttga aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc
     2221 tcggcctgct ctctgatggg cttatcccgg tgcttgttgt aggcggacag cactttgtcc
     2281 agattagcgt cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg
     2341 tccaggtagt gcttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag
     2401 cccttcagct tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg
     2461 gccagttcgt ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt
     2521 tccagctcga acagggagta cttaggcagc ttgatgatca ggtccttttt cacttctttg
     2581 tagcccttgg cttccagaaa gtcgatggga ttcttctcga agctgcttct ttccatgatg
     2641 gtgatcccca gcagctcttt cacactcttc agtttcttgg acttgccctt ttccactttg
     2701 gccaccacca gcacagaata ggccacggtg gggctgtcga agccgccgta cttcttaggg
     2761 tcccagtcct tctttctggc gatcagctta tcgctgttcc tcttgggcag gatagactct
     2821 ttgctgaagc cgcctgtctg cacctcggtc tttttcacga tattcacttg gggcatgctc
     2881 agcactttcc gcacggtggc aaaatcccgg cccttatccc acacgatctc cccggtttcg
     2941 ccgtttgtct cgatcagagg ccgcttccgg atctcgccgt tggccagggt aatctcggtc
     3001 ttgaaaaagt tcatgatgtt gctgtagaag aagtacttgg cggtagcctt gccgatttcc
     3061 tgctcgctct tggcgatcat cttccgcacg tcgtacacct tgtagtcgcc gtacacgaac
     3121 tcgctttcca gcttagggta ctttttgatc agggcggttc ccacgacggc gttcaggtag
     3181 gcgtcgtggg cgtggtggta gttgttgatc tcgcgcactt tgtaaaactg gaaatccttc
     3241 cggaaatcgg acaccagctt ggacttcagg gtgatcactt tcacttcccg gatcagcttg
     3301 tcattctcgt cgtacttagt gttcatccgg gagtccagga tctgtgccac gtgctttgtg
     3361 atctgccggg tttccaccag ctgtctcttg atgaagccgg ccttatccag ttcgctcagg
     3421 ccgcctctct cggccttggt cagattgtcg aactttctct gggtaatcag cttggcgttc
     3481 agcagctgcc gccagtagtt cttcatcttc ttcacgacct cttcggaggg cacgttgtcg
     3541 ctcttgcccc ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga gtcgtccttc
     3601 agaaagctct gaggcacgat atggtccaca tcgtagtcgg acagccggtt gatgtccagt
     3661 tcctggtcca cgtacatatc ccgcccattc tgcaggtagt acaggtacag cttctcgttc
     3721 tgcagctggg tgttttccac ggggtgttct ttcaggatct ggctgcccag ctctttgatg
     3781 ccctcttcga tccgcttcat tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg
     3841 ttctctctgg ccatttcgat cacgatgttc tcgggcttgt gccggcccat cactttcacg
     3901 agctcgtcca ccaccttcac tgtctgcagg atgcccttct taatggcggg gctgccggcc
     3961 agattggcaa tgtgctcgtg caggctatcg ccctggccgg acacctgggc tttctggatg
     4021 tcctctttaa aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag
     4081 ccgtcggact tcaggaaatc caggattgtc ttgccggact gcttgtcccg gatgccgttg
     4141 atcagcttcc ggctcagcct gccccagccg gtgtatctcc gccgcttcag ctgcttcatc
     4201 actttgtcgt cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc
     4261 tcaaacagtg tcagggtcag cacgatatct tccagaatgt cctcgttttc ctcattgtcc
     4321 aggaagtcct tgtccttgat aattttcagc agatcgtggt atgtgcccag ggaggcgttg
     4381 aaccgatctt ccacgccgga gatttccacg gagtcgaagc actcgatttt cttgaagtag
     4441 tcctctttca gctgcttcac ggtcactttc cggttggtct tgaacagcag gtccacgatg
     4501 gcctttttct gctcgccgct caggaaggcg ggctttctca ttccctcggt cacgtatttc
     4561 actttggtca gctcgttata cacggtgaag tactcgtaca gcaggctgtg cttgggcagc
     4621 accttctcgt tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa gctctgggcg
     4681 gaagcgccct tgtccaccac ttcctcgaag ttccaggggg tgatggtttc ctcgctcttt
     4741 ctggtcatcc aggcgaatct gctgtttccc ctggccagag ggcccacgta gtaggggatg
     4801 cggaaggtca ggatcttctc gatcttttcc cggttgtcct tcaggaatgg gtaaaaatct
     4861 tcctgccgcc gcagaatggc gtgcagctct cccaggtgga tctggtgggg gatgctgccg
     4921 ttgtcgaagg tccgctgctt ccgcagcagg tcctctctgt tcagcttcac gagcagttcc
     4981 tcggtgccgt ccatcttttc caggatgggc ttgatgaact tgtagaactc ttcctggctg
     5041 gctccgccgt caatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa aatctctttg
     5101 tacttctcag gcagctgctg ccgcacgaga gctttcagca gggtcaggtc ctggtggtgc
     5161 tcgtcgtatc tcttgatcat agaggcgctc aggggggcct tggtgatctc ggtgttcact
     5221 ctcaggatgt cgctcagcag gatggcgtcg gacaggttct tggcggccag aaacaggtcg
     5281 gcgtactggt cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta ggtgtccttg
     5341 ctcagctgca gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt gggggtcagg
     5401 cccaggctca gggcaatcag gtttccgaac aggccattct tcttctcgcc gggcagctgg
     5461 gcgatcagat tttccagccg tctgctcttg ctcagtctgg cagacaggat ggccttggcg
     5521 tccacgccgc tggcgttgat ggggttttcc tcgaacagct ggttgtaggt ctgcaccagc
     5581 tggatgaaca gcttgtccac gtcgctgttg tcggggttca ggtcgccctc gatcaggaag
     5641 tggccccgga acttgatcat gtgggccagg gccagataga tcagccgcag gtcggccttg
     5701 tcggtgctgt ccaccagttt ctttctcagg tggtagatgg tggggtactt ctcgtggtag
     5761 gccacctcgt ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt atcctcttcc
     5821 accaggaagg actcttccag tctgtggaag aagctgtcgt ccaccttggc catctcgttg
     5881 ctgaagatct cttgcagata gcagatccgg ttcttccgtc tggtgtatct tcttctggcg
     5941 gttctcttca gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag ggctccgatc
     6001 aggttcttct tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt cttgctgggc
     6061 accttgtact cgtcggtgat cacggcccag cccacagagt tggtgccgat gtccaggccg
     6121 atgctgtact tcttgtcggc tgctgggact ccgtggatac cgaccttccg cttcttcttt
     6181 ggggccatgg tggcggcctc agaggccaac agcggccgcg atatcctcga ggaattcttt
     6241 gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta ggaaaaagaa
     6301 gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc agaagccgaa
     6361 ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc cccgaggctg
     6421 gagagggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt
     6481 cctgccggcg ccgcaccgct tcgccccgcg cccgctagag ggggtgcggc ggcgcctccc
     6541 agatttcggc tccgcacaga tttgggacaa aggaagtccc tgcgccctct cgcacgatta
     6601 ccataaaagg caatggctgc ggctcgccgc gcctcgacag ccgccggcgc tccgggggcc
     6661 gccgcgcccc tcccccgagc cctccccggc ccgaggcggc cccgccccgc ccggcacccc
     6721 cacctgccgc caccccccgc ccggcacggc gagccccgcg ccacgccccg tacggagccc
     6781 cgcacccgaa gccgggccgt gctcagcaac tcggggaggg gggtgcaggg ggggttgcag
     6841 cccgaccgac gcgcccacac cccctgctca cccccccacg cacacacccc gcacgcagcc
     6901 tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg cccccggccg cgctcccctc
     6961 gcgcacactg cggagcgcac aaagccccgc gccgcgcccg cagcgctcac agccgccggg
     7021 cagcgcggag ccgcacgcgg cgctccccac gcacacacac acgcacgcac cccccgagcc
     7081 gctccccccg cacaaagggc cctcccggag cccctcaagg ctttcacgca gccacagaaa
     7141 agaaacaagc cgtcattaaa ccaagcgcta attacagccc ggaggagaag ggccgtcccg
     7201 cccgctcacc tgtgggagta acgcggtcag tcagagccgg ggcgggcggc gcgaggcggc
     7261 ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact cccgcccgcc gcgcgcttcg
     7321 ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc ggagcgcgcc
     7381 gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct cgccccgccc
     7441 cgccccgcct gngcgcgccc cccccccccc cccgccccca tcgctgcaca aaataattaa
     7501 aaaataaata aatacaaaat tgggggtggg gagggggggg agatggggag agtgaagcag
     7561 aacgtggggc tcacctcgac catggtaata gcgatgacta atacgtagat gtactgccaa
     7621 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     7681 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     7741 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     7801 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     7861 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
     7921 gtaattgatt actattaata actagtcaat aatcaatgtc gactcgtatt cccgggctgc
     7981 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     8041 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     8101 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgccggc ccctcccccc
     8161 gccgcgcgcg cgcccgctgc cccgcccttc gtggccgccc ggcgtgggcg gtgccacccc
     8221 tccccccggc ggccccgcgc gcagctcccg gctccctccc ccttcggatg tggcttgagc
     8281 tgtaggcgcg gagggccgga gacgctgcag acccgcgacc cggagcagct cggaggcggt
     8341 gaagtcggtg gctttccttc tctctagctc tcgctcgctg gtggtgcttc agatgccaca
     8401 cgcgtagctt ataataccgg ttcaattgcc gacccctccc cccaacttct cggggactgt
     8461 gggcgatgtg cgctctgccc actgacgggc accggagcct ctaggcgaga ccctgtctca
     8521 caaaataaag taagcccgga ctgagtgcgg aaaggcgggc ctggcgggtc tggtctcccc
     8581 atgcgggcca ccagaggccc tgcagccttc agtcgcttga aggggtaatg gcgcttccac
     8641 tcacaaacat ggcggacaga gcgtgtgaac gagatgaaca gcccctcaaa aatatggccg
     8701 ccgaggctgg acggccgtgc cccagcagca ccgcctccgc gccccacgtg atctctcgcc
     8761 gggcacagcg ctgaccgcgg aggtccaacc ggaagaatgt ccggattgga cattcggaag
     8821 agggcccgcc ttccctgggg aatctctgcg cacgcgcaga acgcttcgac caatgaaaac
     8881 acaggaagcc gtccgcgcaa ccgcgttgcg tcacttctgc cgcccctgtt tcaaggtata
     8941 tagccgtaga cggaacttcg cctttctctc ggccttagcg ccattttttt gggtgagtgt
     9001 tttttggttc ctgcgttggg attccgtgta caatccatag acatctgacc tcggcactta
     9061 gcatcatcac agcaaactaa ctgtagcctt tctctctttc cctgtagaaa cctctgcacc
     9121 tgaggccacc atgttgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt
     9181 gcgcatggag ggctccatga acggccatga gttcgagatc gagggcgagg gcgagggccg
     9241 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     9301 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     9361 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     9421 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     9481 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     9541 gcagaagaag acaatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     9601 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     9661 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     9721 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     9781 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agggcagtgg
     9841 agctactaac ttcagcctgc tgaagcaggc tggtgacgtc gaggagaatc ctggccccat
     9901 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     9961 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
    10021 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    10081 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    10141 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    10201 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    10261 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    10321 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    10381 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    10441 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatt
    10501 cgaaggcctg tcgtgaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
    10561 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
    10621 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
    10681 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
    10741 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat
    10801 gctggctgcc atgaactagc tactcgggac cccttaccga aacatcgccg cattctgcag
    10861 aggagtcgag tgtatgtaaa cttctgaccc actgggaatg tgatgaaaga aataaaagct
    10921 gaaatgaatc attctctcta ctattattct gatatttcac attcttaaaa taaagtggtg
    10981 atcctaactg acctaagaca gggaattttt actaggatta aatgtcagga attgtgaaaa
    11041 agtgagttta aatgtatttg gctaaggtgt atgtaaactt ccgacttcaa ctgtataggg
    11101 atccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11701 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    12001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    12061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    12121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    12301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    13021 agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    13081 ggaaattgta agcgttaata ttttgttaaa att
//
