LOCUS       pTW335c3_-_[pBR322]_Pta 7520 bp ds-DNA     circular     05-JUL-2024
DEFINITION  .
ACCESSION   urn.local...16e-aqel875
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW004c.gb [ColE1]
            Ptet T7RNAP N-term aadA. Source name: pTW002 - AP SC101 RepA Ptet
            SD8 MBP bla tetR (Note: Geneious sometimes stores the sequence name
            in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTW002a -
            CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW002 - AP SC101 RepA Ptet SD8 MBP bla tetR (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     CDS             complement(1..1083)
                     /label="lacI"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        complement(1084..1161)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     USER junction   1166..1179
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        1404..1432
                     /label="tac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     operator        1436..1458
                     /label="lacO"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     USER junction   1515..1523
                     /label="USER009"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             1524..1536
                     /label="RBS"
                     /ApEinfo_revcolor="#82ca9d"
                     /ApEinfo_fwdcolor="#82ca9d"
     CDS             1537..2973
                     /label="AtRbcL ΔN1"
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
     CDS             1537..2973
                     /label="Translation 1537-2973"
                     /translation="MPQTETKASVGFKAGVKEYKLTYYTPEYETKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYHIEPVPGEETQFIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALAALRLEDLRIPPAYTKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEAIYKSQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLSHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRLSGGDHIHAGTVVGKLEGDRESTLGFVDLLRDDYVEKDRSRGIFFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAVEGNEIIREACKWSPELAAACEVWKEITFNFPTIDKLDGQE*"
     RBS             2986..3008
                     /label="RBS"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     CDS             3016..3424
                     /label="AtRbcS 3B"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     CDS             3016..3424
                     /label="Translation 3016-3424"
                     /translation="MKVWPPIGKKKFETLSYLPDLSDVELAKEVDYLLRNKWIPCVEFELEHGFVYREHGNTPGYYDGRYWTMWKLPLFGCTDSAQVLKEVEECKKEYPGAFIRIIGFDNTRQVQCISFIAYKPPSFTEAALEHHHHHH*"
     affinity tag    3403..3420
                     /label="6xHis"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     USER junction   3486..3498
                     /label="USER018"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      3501..3548
                     /label="T7 terminator (pET)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        3774..3968
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     CDS             4167..4826
                     /label="AmpR"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     rep_origin      4924..5606
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     origin          4984..5603
                     /label="pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5774..5916
                     /label="color: #a6acb3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6018..6209)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6306..6347
                     /label="start of unknown region"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tcgatggtgt cgcgcaacgc aattaatgta agttagctca ctcattaggc
     1201 acaattctca tgtttgacag cttatcatcg actgcacggt gcaccaatgc ttctggcgtc
     1261 aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg
     1321 tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca taacggttct
     1381 ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat
     1441 tgtgagcgga taacaatttc acacaggaaa cagccagtcc gtttaggtgt tttcacgagc
     1501 acttcaccaa caatAGGTGC AGTaaggaga tataccatgc cacaaacaga gactaaagca
     1561 agtgttgggt tcaaagctgg tgttaaagag tataaattga cttactatac tcctgaatat
     1621 gaaaccaagg atactgatat cttggcagca ttccgagtaa ctcctcaacc tggagttcca
     1681 cctgaagaag caggggctgc ggtagctgct gaatcttcta ctggtacatg gacaactgtg
     1741 tggaccgatg ggcttaccag ccttgatcgt tacaaaggac gatgctacca catcgagccc
     1801 gttccaggag aagaaactca atttattgcg tatgtagctt atcccttaga cctttttgaa
     1861 gaaggttcgg ttactaacat gtttacctcg attgtgggta atgtatttgg gttcaaagcc
     1921 ctggctgctc tacgtctaga ggatctgcga atccctcctg cttatactaa aactttccaa
     1981 ggaccacctc atggtatcca agttgaaaga gataaattga acaagtatgg acgtccccta
     2041 ttaggatgta ctattaaacc aaaattgggg ttatccgcga aaaactatgg tagagcagtt
     2101 tatgaatgtc tacgtggtgg acttgatttt accaaagatg atgagaatgt gaactcccaa
     2161 ccatttatgc gttggagaga ccgtttctta ttttgtgccg aagctattta taaatcacag
     2221 gctgaaacag gtgaaatcaa agggcattat ttgaatgcta ctgcgggtac atgcgaagaa
     2281 atgatcaaaa gagctgtatt tgccagagaa ttgggagttc ctatcgtaat gcatgactac
     2341 ttaacagggg gattcaccgc aaatactagt ttgtctcatt attgccgaga taatggccta
     2401 cttcttcaca tccaccgtgc aatgcacgct gttattgata gacagaagaa tcatggtatg
     2461 cacttccgtg tactagctaa agctttacgt ctatctggtg gagatcatat tcacgcgggt
     2521 acagtagtag gtaaacttga aggagacagg gagtcaactt tgggctttgt tgatttactg
     2581 cgcgatgatt atgttgaaaa agatcgaagc cgcggtatct ttttcactca agattgggtc
     2641 tcactacctg gtgttctgcc tgtggcttca gggggtattc acgtttggca catgcctgct
     2701 ttgaccgaga tctttggaga tgattctgta ctacaattcg gtggaggaac tttaggccac
     2761 ccttggggaa atgcaccggg tgccgtagcc aaccgagtag ctctggaagc atgtgtacaa
     2821 gctcgtaatg agggacgtga tcttgcagtc gagggtaatg aaattatccg tgaagcttgc
     2881 aaatggagtc ctgaactagc tgctgcttgt gaagtatgga aagagatcac atttaacttc
     2941 ccaaccatcg ataaattaga tggccaagag taaGCTAGAA ATAATTTTGT TTAACTTTAA
     3001 GAAGGAGATA TACATATGAA GGTGTGGCCA CCAATTGGAA AGAAGAAGTT TGAGACTCTA
     3061 TCTTACCTCC CTGACCTTAG TGACGTCGAA TTGGCTAAGG AAGTTGACTA CCTTCTCCGC
     3121 AACAAGTGGA TTCCTTGTGT TGAATTCGAG TTAGAGCACG GATTTGTGTA CCGTGAGCAC
     3181 GGAAACACTC CCGGATACTA CGATGGACGG TACTGGACAA TGTGGAAGCT TCCATTGTTC
     3241 GGATGCACCG ACTCCGCTCA AGTGTTGAAG GAAGTTGAAG AATGCAAGAA GGAGTACCCG
     3301 GGCGCCTTCA TTAGGATCAT CGGATTCGAC AACACCCGTC AAGTCCAATG CATCAGTTTC
     3361 ATTGCCTACA AGCCCCCAAG CTTCACCGAA GCTGCACTCG AGCACCACCA CCACCACCAC
     3421 TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT GGCTGCTGCC ACCGCTGAGC
     3481 AATAAaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     3541 gttttttgct gaaaggagga actatatccg gatatcccgc aagaggcccg gcagtaccgg
     3601 cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc
     3661 attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc
     3721 gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc
     3781 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3841 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3901 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3961 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4021 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4081 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4141 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4201 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4261 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4321 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4381 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4441 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4501 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4561 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4621 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4681 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4741 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4801 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4861 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4921 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5641 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5701 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5761 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5821 gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     5881 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     5941 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6001 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     6061 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     6121 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     6181 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     6241 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     6301 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     6361 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     6421 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     6481 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     6541 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     6601 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     6661 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     6721 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     6781 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     6841 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     6901 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     6961 cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga
     7021 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     7081 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     7141 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     7201 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     7261 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     7321 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     7381 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     7441 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     7501 ttacattaat tgcgttgcgc
//