LOCUS       pTW335f3_-_[pBR322]_Pta 7504 bp ds-DNA     circular     05-JUL-2024
DEFINITION  .
ACCESSION   urn.local...16e-aqel875
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW004c.gb [ColE1]
            Ptet T7RNAP N-term aadA. Source name: pTW002 - AP SC101 RepA Ptet
            SD8 MBP bla tetR (Note: Geneious sometimes stores the sequence name
            in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTW002a -
            CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW002 - AP SC101 RepA Ptet SD8 MBP bla tetR (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     misc_feature    455..597
                     /label="color: #a6acb3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(699..890)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2202..3284)
                     /label="lacI"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        complement(3285..3362)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     USER junction   3367..3380
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        3605..3633
                     /label="tac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     operator        3637..3659
                     /label="lacO"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     USER junction   3716..3724
                     /label="USER009"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             3725..3737
                     /label="RBS"
                     /ApEinfo_revcolor="#82ca9d"
                     /ApEinfo_fwdcolor="#82ca9d"
     gene            3738..5162
                     /label="GmRbcL ΔN1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3738..5162
                     /label="Translation 3738-5162"
                     /translation="MPQTETKASVGFKAGVKDYKLTYYTPDYETKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYGLEPVAGEENQYIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPTAYIKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEAIFKSQAETGEIKGHYLNATAGTCEEMMKRAVFARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRLSGGDHVHAGTVVGKLEGEREITLGFVDLLRDDFVEKDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAREGNEIIREASKWSPELAAACEVWKEIKFEFEAMDTL*"
     RBS             5175..5197
                     /label="RBS"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     gene            5205..5579
                     /label="GmRbcS1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             5205..5609
                     /label="Translation 5205-5609"
                     /translation="MCMQVWPPIGKKKFETLSYLPDLDDAQLAKEVEYLLRKGWIPCLEFELEHGFVYREHNRSPGYYDGRYWTMWKLPMFGCTDASQVLKELQEAKTAYPNGFIRIIGFDNVRQVQCISFIAYKPPGFALEHHHHHH*"
     CDS             5580..5588
                     /label="spacer"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     misc_feature    5589..5606
                     /label="6xHis"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     USER junction   5610..5620
                     /label="USER023"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      5686..5733
                     /label="T7 terminator (pET)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        5959..6153
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     CDS             6352..7011
                     /label="AmpR"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     rep_origin      7109..287
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     origin          7169..284
                     /label="pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
       61 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      421 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      481 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      721 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
      781 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      841 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      901 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      961 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     1021 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     1081 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     1141 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     1201 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     1261 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     1321 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     1381 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     1441 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     1501 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     1561 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     1621 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     1681 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     1741 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     1801 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     1861 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     1921 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     1981 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     2041 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     2101 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     2161 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     2221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2281 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     2341 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     2401 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     2461 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     2521 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     2581 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     2641 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     2701 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     2761 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     2821 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     2881 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     2941 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3001 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     3061 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     3121 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     3181 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     3241 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     3301 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     3361 tcgcgcaacg caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca
     3421 gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     3481 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
     3541 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     3601 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     3661 cacacaggaa acagccagtc cgtttaggtg ttttcacgag cacttcacca acaatAGGTG
     3721 CAGTaaggag atataccatg ccacaaacag agactaaagc aagtgttggg ttcaaagctg
     3781 gtgttaaaga ttataaattg acttattata ctcctgacta tgaaaccaaa gatactgata
     3841 tcttggcagc attccgagta actcctcaac caggagttcc gcctgaagaa gcaggtgccg
     3901 cggtagccgc cgaatcttct actggtacat ggacaactgt gtggaccgat gggcttacca
     3961 gtcttgatcg ttacaaaggg cgatgctacg gccttgaacc tgttgctggg gaagaaaatc
     4021 aatatattgc ttatgtagct tatcccttag acctttttga agaaggttct gttactaaca
     4081 tgtttacttc cattgtcggt aatgtatttg ggttcaaggc cctgcgtgct ctacgtctgg
     4141 aggatttgcg aatccctact gcttatatta aaactttcca aggtccgcct catggcatcc
     4201 aagttgagag agataaattg aacaagtatg gtcgtcccct attaggatgt actattaaac
     4261 ctaaattggg gttatccgct aagaattatg gtagagctgt ttatgaatgt cttcgtgggg
     4321 gacttgattt taccaaagat gatgaaaatg tgaattccca accatttatg cgttggagag
     4381 accgtttctt attttgtgcc gaagccattt ttaaatcaca ggctgaaaca ggtgaaatca
     4441 aagggcatta cttgaatgca actgcgggta catgcgaaga aatgatgaaa agagctgtat
     4501 ttgccagaga attaggcgtt cctatcgtaa tgcatgatta tttaacgggg ggattcactg
     4561 caaatactag cttggctcat tattgccgag ataatggtct acttcttcat atacaccgtg
     4621 caatgcatgc agttatcgac agacaaaaga atcatggtat gcactttcgt gtactagcta
     4681 aagcattacg tttgtctggt ggagatcatg ttcacgccgg taccgtagta ggtaaacttg
     4741 aaggggaaag agaaatcact ttaggttttg ttgatttact acgtgatgat tttgttgaaa
     4801 aagatcgaag tcgcggtatt tatttcactc aggattgggt ttctctacca ggtgttttgc
     4861 ctgtagcttc gggaggtatt cacgtttggc atatgcctgc tctgaccgag atctttgggg
     4921 atgattctgt actccaattt ggcggaggaa ctttaggaca cccttgggga aatgcaccag
     4981 gtgctgtagc taatcgagta gctcttgaag catgtgtgca ggctcgaaat gaaggacgcg
     5041 atcttgctcg tgaaggtaat gaaattatcc gtgaggctag caaatggagt cctgaattag
     5101 ctgctgcttg tgaagtatgg aaggagatca aatttgaatt cgaagcaatg gatactttgt
     5161 aagctAGAAA TAATTTTGTt taactttaag aaggagatat acatatgtgt atgcaagttt
     5221 ggccccccat cggtaagaaa aaatttgaga ccctgtcata cttgccagac ctggatgatg
     5281 cccagttagc taaagaagtg gaatatcttc tccgaaaagg ttggattccg tgtctggaat
     5341 ttgagttaga gcatggcttc gtttaccgcg aacacaaccg tagtccgggg tattatgacg
     5401 gccgttactg gactatgtgg aaactgccta tgttcggatg caccgatgcg agccaggtcc
     5461 taaaagaact gcaagaagcg aagacggcct atccgaacgg cttcatccgc attattggct
     5521 ttgataatgt gcgccaggta caatgtattt cgtttatagc atacaaaccg cctggttttg
     5581 cACTCGAGCA Tcaccaccat catcattaaa tccggctgct aacaaagccc gaaaggaagc
     5641 tgagtggctg ctgccaccgc tgagcaataa accgctgagc aataactagc ataacccctt
     5701 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
     5761 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
     5821 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
     5881 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
     5941 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6001 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6061 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6121 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6241 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6301 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6361 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     6421 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6481 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6541 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6601 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6661 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     6721 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6781 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6841 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6901 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6961 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7021 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7081 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7141 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7321 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7501 tgaa
//