LOCUS       pTW335g3_-_[pBR322]_Pta 7510 bp ds-DNA     circular     05-JUL-2024
DEFINITION  .
ACCESSION   urn.local...16e-aqel875
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW004c.gb [ColE1]
            Ptet T7RNAP N-term aadA. Source name: pTW002 - AP SC101 RepA Ptet
            SD8 MBP bla tetR (Note: Geneious sometimes stores the sequence name
            in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTW002a -
            CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW002 - AP SC101 RepA Ptet SD8 MBP bla tetR (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     misc_feature    455..597
                     /label="color: #a6acb3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(699..890)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2202..3284)
                     /label="lacI"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        complement(3285..3362)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     promoter        3605..3633
                     /label="tac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     operator        3637..3659
                     /label="lacO"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     RBS             3725..3737
                     /label="RBS"
                     /ApEinfo_revcolor="#82ca9d"
                     /ApEinfo_fwdcolor="#82ca9d"
     gene            3738..5168
                     /label="StRbcL ΔN1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3738..5168
                     /label="Translation 3738-5168"
                     /translation="MPQTETKASVGFKAGVKEYKLTYYTPEYQTKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYRIERVVGEKDQYIAYVAYPLDLFEEGSVTNMLTSIVGNVFGFKALRALRLEDLRIPVAYVKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEALFKAQAETGEIKGHYLNATAGTCEEMMKRAVFARELGTPIVMHDYLTGGFTANTTLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRMSGGDHIHSGTVVGKLEGERDITLGFVDLLRDDFIEQDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVKARNEGRDLAREGNEIIREAAKWSPELAAACEVWKEIVFNFAAMDVLDK*"
     RBS             5181..5203
                     /label="RBS"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     gene            5211..5615
                     /label="StRbcS1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             5211..5615
                     /label="Translation 5211-5615"
                     /translation="MCMQVWPPINMKKYETLSYLPDLSDEQLLKEVEYLLKNGWVPCLEFETEHGFVYREHNSSPGYYDGRYWTMWKLPMFGCTDGTQVLAEVQEAKNAYPQAWIRIIGFDNVRQVQCISFIAYKPEGYALEHHHHHH*"
     CDS             5586..5594
                     /label="spacer"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     misc_feature    5595..5612
                     /label="6xHis"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     terminator      5692..5739
                     /label="T7 terminator (pET)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        5965..6159
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     CDS             6358..7017
                     /label="AmpR"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     rep_origin      7115..287
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     origin          7175..284
                     /label="pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
       61 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      421 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      481 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      721 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
      781 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      841 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      901 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      961 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     1021 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     1081 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     1141 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     1201 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     1261 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     1321 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     1381 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     1441 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     1501 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     1561 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     1621 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     1681 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     1741 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     1801 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     1861 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     1921 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     1981 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     2041 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     2101 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     2161 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     2221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2281 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     2341 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     2401 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     2461 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     2521 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     2581 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     2641 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     2701 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     2761 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     2821 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     2881 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     2941 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3001 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     3061 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     3121 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     3181 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     3241 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     3301 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     3361 tcgcgcaacg caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca
     3421 gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     3481 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
     3541 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     3601 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     3661 cacacaggaa acagccagtc cgtttaggtg ttttcacgag cacttcacca acaatAGGTG
     3721 CAGTaaggag atataccatg ccacaaacag agactaaagc aagtgttgga ttcaaagctg
     3781 gtgttaaaga gtacaaattg acttattata ctcctgagta ccaaaccaag gatactgata
     3841 tattggcagc attccgagta actcctcaac ctggagttcc acctgaagaa gcaggggccg
     3901 cggtagctgc cgaatcttct actggtacat ggacaactgt atggaccgat ggacttacca
     3961 gtcttgatcg ttacaaaggg cgatgctacc gcatcgagcg tgttgttgga gaaaaagatc
     4021 aatatattgc ttatgtagct taccctttag acctttttga agaaggttcc gttaccaata
     4081 tgcttacttc cattgtaggt aacgtatttg ggttcaaagc cctgcgcgct ctacgtctgg
     4141 aagatctgcg aatccctgtt gcttatgtta aaactttcca aggcccgcct catgggatcc
     4201 aagttgaaag agataaattg aacaagtatg gtcgtcccct gttgggatgt actattaaac
     4261 ctaaattggg gttatctgca aaaaactacg gtagagctgt ttatgaatgt cttcgcggtg
     4321 gacttgattt taccaaagat gatgagaacg tgaactcaca accatttatg cgttggagag
     4381 atcgtttctt attttgtgcc gaagcacttt ttaaagcaca ggctgaaaca ggtgaaatca
     4441 aagggcatta cttgaatgct actgcaggta catgcgaaga aatgatgaaa agagctgtat
     4501 ttgctagaga attgggcact ccgatcgtaa tgcatgacta cttaacgggg ggattcaccg
     4561 caaatactac cttggctcat tattgccgag ataatggtct acttcttcac atccaccgtg
     4621 caatgcatgc ggttattgat agacagaaga atcatggtat gcacttccgg gtattagcaa
     4681 aagcgttacg tatgtctggt ggagatcata ttcactctgg taccgtagta ggtaaacttg
     4741 aaggtgaaag agacataact ttgggctttg ttgatttact gcgtgatgat tttattgaac
     4801 aagatagaag tcgcggtatt tatttcactc aagattgggt ctctttacca ggtgttctac
     4861 ctgtggcttc aggaggtatt cacgtttggc atatgcctgc tctgaccgag atctttgggg
     4921 atgattccgt actacagttc ggtggaggaa ctttaggaca tccttggggt aatgcgccag
     4981 gtgccgtagc taatcgagta gctctagaag catgtgtaaa agctcgtaat gaaggacgtg
     5041 atcttgctcg ggaaggtaat gagattattc gcgaggctgc caaatggagc ccggaactag
     5101 ctgctgcttg tgaggtatgg aaagagatcg tatttaattt tgcagcaatg gacgttttgg
     5161 ataagtaagc tAGAAATAAT TTTGTttaac tttaagaagg agatatacat atgtgtatgc
     5221 aagtttggcc cccgattaac atgaaaaaat atgagaccct gagctacttg ccggacctgt
     5281 cggatgaaca gttactcaaa gaagtggaat atctgctgaa gaacggctgg gtgccttgcc
     5341 tggagttcga gacggaacat ggttttgtct atcgtgaaca caatagctca ccgggctatt
     5401 acgacggacg ttactggaca atgtggaagc ttccaatgtt cggttgcacc gatggcactc
     5461 aggttttagc tgaagtacag gaagcaaaaa acgcgtatcc gcaagcgtgg attcgcatca
     5521 ttggctttga taatgtccgc caggtgcagt gtatcagttt tattgcctac aaaccggaag
     5581 ggtacgcACT CGAGCATcac caccatcatc attaaATCCG GCTGCTaaca aagcccgaaa
     5641 ggaagctgag tggctgctgc caccgctgag caataaaccg ctgagcaata actagcataa
     5701 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     5761 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     5821 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     5881 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
     5941 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6001 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6061 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6121 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6181 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6241 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6301 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6361 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     6421 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6481 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6541 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6601 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6661 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     6721 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6781 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6841 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6901 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6961 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7021 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7081 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7201 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7261 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7321 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7381 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7441 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7501 tcgggctgaa
//