LOCUS       pTW335h3_-_[pBR322]_Pta 7525 bp ds-DNA     circular     05-JUL-2024
DEFINITION  .
ACCESSION   urn.local...16e-aqel875
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW004c.gb [ColE1]
            Ptet T7RNAP N-term aadA. Source name: pTW002 - AP SC101 RepA Ptet
            SD8 MBP bla tetR (Note: Geneious sometimes stores the sequence name
            in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTW002a -
            CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW002 - AP SC101 RepA Ptet SD8 MBP bla tetR (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     misc_feature    455..597
                     /label="color: #a6acb3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(699..890)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2202..3284)
                     /label="lacI"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        complement(3285..3362)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     promoter        3605..3633
                     /label="tac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     operator        3637..3659
                     /label="lacO"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     RBS             3725..3737
                     /label="RBS"
                     /ApEinfo_revcolor="#82ca9d"
                     /ApEinfo_fwdcolor="#82ca9d"
     gene            3738..5168
                     /label="OsjRbcL ΔN1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3738..5168
                     /label="Translation 3738-5168"
                     /translation="MPQTETKASVGFKAGVKDYKLTYYTPEYETKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYHIEPVVGEDNQYIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPTYSKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRACYECLRGGLDFTKDDENVNSQPFMRWRDRFVFCAEAIYKSQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRMSGGDHIHAGTVVGKLEGEREMTLGFVDLLRDDFIEKDRARGIFFTQDWVSMPGVIPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAAANRVALEACVQARNEGRDLAREGNEIIRSACKWSPELAAACEIWKAIKFEFEPVDKLDS*"
     RBS             5181..5203
                     /label="RBS"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     gene            5211..5630
                     /label="OsjRbcS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5211..5630
                     /label="Translation 5211-5630"
                     /translation="MCMQVWPIEGIKKFETLSYLPPLTVEDLLKQIEYLLRSKWVPCLEFSKVGFVYRENHRSPGYYDGRYWTMWKLPMFGCTDATQVLKELEEAKKAYPDAFVRIIGFDNVRQVQLISFIAYKPPGCEESGGNALEHHHHHH*"
     CDS             5601..5609
                     /label="spacer"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     misc_feature    5610..5627
                     /label="6xHis"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     terminator      5707..5754
                     /label="T7 terminator (pET)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        5980..6174
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     CDS             6373..7032
                     /label="AmpR"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     rep_origin      7130..287
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     origin          7190..284
                     /label="pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
       61 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      421 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      481 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      721 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
      781 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      841 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      901 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      961 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     1021 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     1081 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     1141 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     1201 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     1261 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     1321 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     1381 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     1441 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     1501 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     1561 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     1621 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     1681 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     1741 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     1801 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     1861 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     1921 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     1981 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     2041 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     2101 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     2161 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     2221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2281 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     2341 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     2401 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     2461 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     2521 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     2581 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     2641 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     2701 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     2761 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     2821 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     2881 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     2941 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3001 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     3061 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     3121 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     3181 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     3241 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     3301 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     3361 tcgcgcaacg caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca
     3421 gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     3481 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
     3541 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     3601 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     3661 cacacaggaa acagccagtc cgtttaggtg ttttcacgag cacttcacca acaataggtg
     3721 cagtaaggag atataccatg ccacaaacag agactaaagc aagtgttgga tttaaagctg
     3781 gtgttaagga ttataaattg acttactaca ccccggagta cgaaaccaag gacactgata
     3841 tcttggcagc attccgagta actcctcagc cgggggttcc gcccgaagaa gcaggggctg
     3901 cagtagctgc cgaatcttct actggtacat ggacaactgt ttggactgat ggacttacca
     3961 gtcttgatcg ttacaaaggc cgatgctatc acatcgagcc cgttgttggg gaggataatc
     4021 aatatatcgc ttatgtagct tatccattag acctatttga agagggttct gttactaaca
     4081 tgtttacttc cattgtgggt aacgtatttg gtttcaaagc cctacgcgct ctacgtctgg
     4141 aggatctgcg aattccccct acttattcaa aaactttcca aggtccgcct catggtatcc
     4201 aagttgaaag ggataagttg aacaaatacg gtcgtccttt attgggatgt actattaaac
     4261 caaaattggg attatctgca aaaaattatg gtagagcatg ttatgagtgt ctacgcggtg
     4321 gacttgattt taccaaagat gatgaaaacg taaactcaca accatttatg cgttggaggg
     4381 accgttttgt cttttgtgcc gaagctattt ataaatcaca ggccgaaacc ggtgaaatta
     4441 aggggcatta cttgaatgcg actgcaggta catgcgaaga aatgattaaa agagctgtat
     4501 ttgcgaggga attaggggtt cctattgtaa tgcatgacta cttaaccggg gggttcaccg
     4561 caaatactag tttggctcat tattgccgcg acaacggcct acttcttcac attcaccgag
     4621 caatgcatgc agttattgat agacagaaaa atcatggtat gcatttccgt gtattagcta
     4681 aagcattgcg tatgtctggg ggagatcata tccacgctgg tacagtagta ggtaagttag
     4741 aaggggaacg cgaaatgact ttaggttttg ttgatttatt gcgcgatgat tttattgaaa
     4801 aagatcgtgc tcgcggtatc tttttcactc aggactgggt atccatgcca ggtgttatac
     4861 cggtggcttc agggggtatt catgtttggc atatgccagc tctgaccgaa atctttggag
     4921 atgattctgt attgcaattt ggtggaggaa ctttaggaca tccttggggt aatgcacctg
     4981 gtgcagcagc taatcgggtg gctttagaag cctgtgtaca agctcgtaac gaagggcgcg
     5041 atcttgctcg tgaaggtaat gaaattatcc gatcagcttg caaatggagt cctgaactag
     5101 ccgcagcttg tgaaatatgg aaagcgatca aattcgagtt cgagccggta gataaactag
     5161 atagctaggc tAGAAATAAT TTTGTttaac tttaagaagg agatatacat atgtgtatgc
     5221 aagtttggcc cattgaaggt ataaaaaaat ttgaaaccct gtcatactta cccccgctga
     5281 cggtcgaaga ccttctaaag caaatcgagt atctgctccg atcgaagtgg gttccatgcc
     5341 tggaattcag taaagtgggt tttgtatacc gtgaaaacca tcgtagcccg gggtattacg
     5401 atggccgcta ttggactatg tggaagctgc cgatgttcgg ctgtaccgat gccacacagg
     5461 tgttgaaaga gctggaggaa gccaaaaaag cgtacccgga tgcgttcgtt cgcattatcg
     5521 gctttgacaa cgtgcgccag gtccaattaa tttcctttat tgcatataaa cctcctggct
     5581 gcgaagaaag cggaggtaat gcACTCGAGC ATcaccacca tcatcattaa ATCCGGCTGC
     5641 Taacaaagcc cgaaaggaag ctgagtggct gctgccaccg ctgagcaata aaccgctgag
     5701 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     5761 ggaggaacta tatccggata tcccgcaaga ggcccggcag taccggcata accaagccta
     5821 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     5881 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat
     5941 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
     6001 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6061 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6121 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6181 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6241 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6301 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6361 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     6421 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6481 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6541 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6601 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6661 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     6721 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6781 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6841 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6901 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6961 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7021 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7201 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7261 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7321 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7381 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7441 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7501 gataaggcgc agcggtcggg ctgaa
//