LOCUS       pTW335p3_[pBR322]_Ptac_ 7498 bp ds-DNA     circular     05-JUL-2024
DEFINITION  .
ACCESSION   urn.local...16e-aqel875
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW004c.gb [ColE1]
            Ptet T7RNAP N-term aadA. Source name: pTW002 - AP SC101 RepA Ptet
            SD8 MBP bla tetR (Note: Geneious sometimes stores the sequence name
            in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTW002a -
            CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW002 - AP SC101 RepA Ptet SD8 MBP bla tetR (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     CDS             complement(699..890)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2202..3284)
                     /label="lacI"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        complement(3285..3362)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     promoter        3605..3633
                     /label="tac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     operator        3637..3659
                     /label="lacO"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     RBS             3725..3737
                     /label="RBS"
                     /ApEinfo_revcolor="#82ca9d"
                     /ApEinfo_fwdcolor="#82ca9d"
     CDS             3738..3755
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MPQTET"
     misc_feature    3756..5162
                     /label="MzRbcL Δ1 N "
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3756..5162
                     /label="MzRbcL"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="KASVGFKAGVKDYKLTYYTPEYETKDTDILAAFRVTPQLGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYHIEPVPGDPDQYICYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPAYSKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRACYECLRGGLDFTKDDENVNSQPFMRWRDRFVFCAEAIYKAQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTTLSHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRMSGGDHIHSGTVVGKLEGEREITLGFVDLLRDDFIEKDRSRGIFFTQDWVSMPGVIPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAAANRVALEACVQARNEGRDLAREGNEIIKAACKWSAELAAACEIWKEIKFDGFKAMDTI"
     CDS             5163..5165
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="*"
     RBS             5178..5200
                     /label="RBS"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     CDS             5208..5228
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MCMQVWP"
     misc_feature    5229..5582
                     /label="MzRbcS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             5229..5603
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="AYGNKKFETLSYLPPLSTDDLLKQVDYLLRNGWIPCLEFSKVGFVYRENSTSPCYYDGRYWTMWKLPMFGCNDATQVYKELQEAIKSYPDAFHRVIGFDNIKQTQCVSFIAYKPPGSDHHHHHH*"
     misc_feature    5583..5600
                     /label="6xHis"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     terminator      5680..5727
                     /label="T7 terminator (pET)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        5953..6147
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     CDS             6346..7005
                     /label="AmpR"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     origin          7163..284
                     /label="pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
       61 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      421 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      481 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      721 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
      781 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      841 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      901 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      961 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     1021 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     1081 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     1141 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     1201 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     1261 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     1321 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     1381 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     1441 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     1501 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     1561 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     1621 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     1681 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     1741 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     1801 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     1861 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     1921 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     1981 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     2041 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     2101 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     2161 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     2221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2281 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     2341 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     2401 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     2461 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     2521 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     2581 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     2641 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     2701 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     2761 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     2821 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     2881 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     2941 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3001 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     3061 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     3121 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     3181 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     3241 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     3301 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     3361 tcgcgcaacg caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca
     3421 gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     3481 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
     3541 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     3601 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     3661 cacacaggaa acagccagtc cgtttaggtg ttttcacgag cacttcacca acaatAGGTG
     3721 CAGTaaggag atataccatg ccacaaacag agactAAAGC GTCCGTAGGG TTTAAGGCGG
     3781 GTGTGAAAGA TTATAAATTG ACCTATTATA CCCCGGAATA TGAAACGAAA GATACCGACA
     3841 TCCTGGCCGC CTTCCGGGTT ACCCCGCAAC TGGGCGTTCC TCCAGAGGAA GCGGGGGCGG
     3901 CGGTGGCCGC TGAAAGCTCC ACGGGAACCT GGACAACTGT GTGGACCGAT GGGCTGACTA
     3961 GCTTAGACCG TTACAAAGGT CGTTGTTATC ATATCGAGCC GGTACCTGGC GACCCTGATC
     4021 AGTATATTTG TTATGTGGCA TATCCCTTGG ATCTGTTCGA AGAGGGAAGC GTAACCAACA
     4081 TGTTTACGAG TATCGTGGGC AACGTTTTCG GCTTTAAGGC ATTGCGTGCG CTACGCTTGG
     4141 AAGATCTGCG AATTCCACCG GCTTACTCAA AAACCTTTCA GGGTCCGCCC CACGGCATTC
     4201 AGGTGGAAAG AGACAAGCTG AACAAGTACG GCAGACCGCT CCTTGGCTGT ACAATCAAAC
     4261 CGAAATTAGG CCTCTCCGCG AAAAACTATG GCCGCGCTTG TTATGAATGC CTGCGTGGTG
     4321 GCCTGGATTT CACGAAAGAC GATGAAAATG TAAATAGCCA GCCATTCATG CGCTGGAGGG
     4381 ATCGCTTTGT GTTTTGCGCG GAGGCCATTT ACAAGGCGCA AGCAGAAACC GGCGAAATTA
     4441 AAGGGCACTA CTTAAATGCT ACGGCCGGTA CTTGTGAGGA AATGATCAAA CGCGCCGTCT
     4501 TTGCCCGTGA ATTGGGAGTT CCTATCGTAA TGCATGACTA CCTGACCGGC GGGTTCACGG
     4561 CCAACACCAC ACTTAGTCAT TACTGCCGCG ATAACGGTTT ACTGCTGCAC ATTCACCGGG
     4621 CCATGCATGC CGTTATTGAT CGTCAGAAAA ATCATGGGAT GCATTTTCGT GTCCTGGCGA
     4681 AAGCACTGCG CATGAGTGGT GGCGATCACA TTCATTCGGG AACTGTCGTT GGCAAACTGG
     4741 AAGGTGAACG CGAAATTACC CTGGGATTTG TTGACTTACT GCGTGACGAC TTCATCGAGA
     4801 AAGATCGGTC ACGCGGCATC TTTTTCACAC AGGACTGGGT GTCTATGCCG GGAGTGATCC
     4861 CCGTTGCATC TGGCGGGATT CATGTCTGGC ACATGCCGGC ACTTACTGAA ATCTTCGGCG
     4921 ATGATTCGGT CCTTCAATTT GGTGGTGGGA CCCTGGGTCA TCCTTGGGGT AATGCACCGG
     4981 GTGCAGCAGC GAACCGTGTG GCTCTGGAAG CGTGCGTGCA AGCTCGCAAT GAAGGACGAG
     5041 ATCTGGCGCG TGAGGGTAAT GAGATCATTA AAGCCGCTTG CAAATGGAGC GCCGAATTGG
     5101 CGGCAGCGTG CGAGATATGG AAAGAAATTA AGTTTGATGG CTTTAAAGCG ATGGATACTA
     5161 TATAAgctAG AAATAATTTT GTttaacttt aagaaggaga tatacatatg TGTATGCAAG
     5221 TTTGGCCCGC TTATGGTAAC AAAAAATTCG AGACTCTGTC CTACCTACCA CCGCTCTCGA
     5281 CGGACGATCT GCTGAAACAA GTGGACTATT TACTTCGGAA CGGCTGGATT CCCTGTCTGG
     5341 AGTTTTCTAA GGTCGGCTTT GTTTATCGCG AAAATAGTAC AAGTCCGTGC TATTACGACG
     5401 GCCGCTACTG GACCATGTGG AAATTGCCGA TGTTCGGTTG TAACGATGCG ACGCAGGTAT
     5461 ACAAAGAACT GCAGGAAGCG ATCAAAAGCT ATCCTGATGC CTTTCATCGT GTGATTGGAT
     5521 TCGATAATAT TAAGCAGACC CAATGCGTGA GCTTTATCGC ATATAAACCG CCAGGGTCAG
     5581 ATcatcacca ccatcatcat TAAATCCGGC TGCTaacaaa gcccgaaagg aagctgagtg
     5641 gctgctgcca ccgctgagca ataaaccgct gagcaataac tagcataacc ccttggggcc
     5701 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca
     5761 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
     5821 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
     5881 ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat
     5941 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6001 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6061 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6121 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6181 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6241 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6301 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6361 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6421 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6481 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6541 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6601 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6661 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     6721 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6781 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6841 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6901 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6961 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7021 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7081 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7141 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7201 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7261 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7321 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7381 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7441 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaa
//